YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction
Han, XY; Yu, FB; Song, XY; Chen, LX; Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. Email:lxchen@yic.ac.cn
Source PublicationCHEMICAL SCIENCE
ISSN2041-6520
2016
Volume7Issue:8Pages:5098-5107
DOI10.1039/c6sc00838k
Contribution Rank[Han, Xiaoyue; Yu, Fabiao; Song, Xinyu; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Han, Xiaoyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Department中科院海岸带环境过程与生态修复重点实验室
AbstractCysteine hydropersulfide (Cys-SSH) plays primary roles in the synthesis of sulfur-containing cofactors, regulation of cellular signaling, activation or inactivation of enzyme activities, and modulation of cellular redox milieu. However, its biofunctions need to be further addressed due to the fact that many issues remain to be clarified. Herein, we conceive a novel ratiometric near-infrared fluorescent probe Cy-Dise for the sensitive and selective detection of Cys-SSH in living cells and in vivo for the first time. Cy-Dise is composed of three moieties: bis(2-hydroxyethyl) diselenide, heptamethine cyanine, and D-galactose. Cy-Dise exhibits a satisfactory linear ratio response to Cys-SSH via a selenium-sulfur exchange reaction in the range of 0-12 mu M Cys-SSH. The experimental detection limit is determined to be 0.12 mu M. The results of ratio imaging analyses confirm the qualitative and quantitative detection capabilities of Cy-Dise in HepG2 cells, HL-7702 cells, and primary hepatocytes. The level changes of Cys-SSH in cells stimulated by some related reagents are also observed. The probe is also suitable for deep tissue ratio imaging. Organ targeting tests with Cy-Dise in normal Spraque-Dawley (SD) rats and Walker-256 tumor SD rats verify its predominant localization in the liver. The probe is promising for revealing the roles of Cys-SSH in physiological and pathological processes.
Funding Organizationprogram of Youth Innovation Promotion Association, CAS(2015170)
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordHIGHLY SELECTIVE DETECTION ; PROTEIN S-SULFHYDRATION ; LIVING CELLS ; IN-VIVO ; HYDROGEN POLYSULFIDES ; OXIDATIVE STRESS ; BIOSYNTHETIC PATHWAYS ; TISSUE DISTRIBUTION ; BIOLOGICAL-SYSTEMS ; HYPOCHLOROUS ACID
WOS Research AreaChemistry
WOS IDWOS:000380893900036
Citation statistics
Cited Times:63[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/17221
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorChen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. Email:lxchen@yic.ac.cn
Recommended Citation
GB/T 7714
Han, XY,Yu, FB,Song, XY,et al. Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction[J]. CHEMICAL SCIENCE,2016,7(8):5098-5107.
APA Han, XY,Yu, FB,Song, XY,Chen, LX,&Chen, LX .(2016).Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction.CHEMICAL SCIENCE,7(8),5098-5107.
MLA Han, XY,et al."Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction".CHEMICAL SCIENCE 7.8(2016):5098-5107.
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