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	Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction
	Han, XY; Yu, FB; Song, XY; Chen, LX; Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. Email:lxchen@yic.ac.cn
发表期刊	CHEMICAL SCIENCE
ISSN	2041-6520
	2016
卷号	7期号:8页码:5098-5107
DOI	10.1039/c6sc00838k
产权排序	[Han, Xiaoyue; Yu, Fabiao; Song, Xinyu; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Han, Xiaoyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	Cysteine hydropersulfide (Cys-SSH) plays primary roles in the synthesis of sulfur-containing cofactors, regulation of cellular signaling, activation or inactivation of enzyme activities, and modulation of cellular redox milieu. However, its biofunctions need to be further addressed due to the fact that many issues remain to be clarified. Herein, we conceive a novel ratiometric near-infrared fluorescent probe Cy-Dise for the sensitive and selective detection of Cys-SSH in living cells and in vivo for the first time. Cy-Dise is composed of three moieties: bis(2-hydroxyethyl) diselenide, heptamethine cyanine, and D-galactose. Cy-Dise exhibits a satisfactory linear ratio response to Cys-SSH via a selenium-sulfur exchange reaction in the range of 0-12 mu M Cys-SSH. The experimental detection limit is determined to be 0.12 mu M. The results of ratio imaging analyses confirm the qualitative and quantitative detection capabilities of Cy-Dise in HepG2 cells, HL-7702 cells, and primary hepatocytes. The level changes of Cys-SSH in cells stimulated by some related reagents are also observed. The probe is also suitable for deep tissue ratio imaging. Organ targeting tests with Cy-Dise in normal Spraque-Dawley (SD) rats and Walker-256 tumor SD rats verify its predominant localization in the liver. The probe is promising for revealing the roles of Cys-SSH in physiological and pathological processes.
文章类型	Article
资助机构	program of Youth Innovation Promotion Association, CAS(2015170)
收录类别	SCI
语种	英语
关键词[WOS]	HIGHLY SELECTIVE DETECTION ; PROTEIN S-SULFHYDRATION ; LIVING CELLS ; IN-VIVO ; HYDROGEN POLYSULFIDES ; OXIDATIVE STRESS ; BIOSYNTHETIC PATHWAYS ; TISSUE DISTRIBUTION ; BIOLOGICAL-SYSTEMS ; HYPOCHLOROUS ACID
研究领域[WOS]	Chemistry
WOS记录号	WOS:000380893900036
引用统计	被引频次：98[WOS]   [WOS记录]     [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/17221
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. Email:lxchen@yic.ac.cn
推荐引用方式 GB/T 7714	Han, XY,Yu, FB,Song, XY,et al. Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction[J]. CHEMICAL SCIENCE,2016,7(8):5098-5107.
APA	Han, XY,Yu, FB,Song, XY,Chen, LX,&Chen, LX .(2016).Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction.CHEMICAL SCIENCE,7(8),5098-5107.
MLA	Han, XY,et al."Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction".CHEMICAL SCIENCE 7.8(2016):5098-5107.

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