One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum
Tang, ZH; Zhao, JL; Ju, B; Li, WJ; Wen, SH; Pu, Y; Qin, S; Tang, ZH (reprint author), Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China. zhhtang6@163.com
2016-07-01
发表期刊PROTEIN EXPRESSION AND PURIFICATION
ISSN1046-5928
卷号123页码:70-74
产权排序[Tang, Zhihong; Zhao, Jilu; Ju, Bao; Wen, Shaohong] Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China; [Li, Wenjun; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui St, Yantai 264003, Peoples R China; [Pu, Yang] Ludong Univ, Coll Agr, 186 Hongqizhong St, Yantai 264025, Peoples R China
摘要B-phycoerythrin (B-PE) was separated and purified from microalga Porphyridium cruentum using one-step chromatographic method. Phycobiliproteins in P. cruentum was extracted by osmotic shock and initially purified by ultrafiltration. Further purification was carried out with a SOURCE 15Q exchange column and analytical grade B-PE was obtained with a purity ratio (A(545)/A(280)) of 5.1 and a yield of 68.5%. It showed a double absorption peaks at 545 nm and 565 nm and a shoulder peak at 498 nm, and displayed a fluorescence emission maximum at 580 nm. The analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed a bulky band between 18 and 20 kDa which could be assigned to subunits alpha and beta and a low intensity band of 27 kDa assigned to gamma subunit. Our protocol provides attractive alternative to consider for the purification procedure to obtain analytical grade B-PE at commercial level. (C) 2016 Elsevier Inc. All rights reserved.
关键词Porphyridium Cruentum B-phycoerythrin Purification Chromatography
作者部门海岸带生物学与生物资源利用所重点实验室
DOI10.1016/j.pep.2016.01.018
项目资助者Ocean Public Welfare Scientific Research Project, State Oceanic Administration, People's Republic of China(20120527)
收录类别SCI
关键词[WOS]BED ADSORPTION CHROMATOGRAPHY ; R-PHYCOERYTHRIN ; PHYCOBILIPROTEINS ; SEPARATION ; RECOVERY ; ALLOPHYCOCYANIN ; PHYCOBILISOMES ; PHYCOCYANIN ; EXTRACTION ; PROTEINS
文章类型Article
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号WOS:000376221600010
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/17077
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Tang, ZH (reprint author), Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China. zhhtang6@163.com
作者单位1.Yantai Univ, Coll Life Sci
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res
3.Ludong Univ
推荐引用方式
GB/T 7714
Tang, ZH,Zhao, JL,Ju, B,et al. One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum[J]. PROTEIN EXPRESSION AND PURIFICATION,2016,123:70-74.
APA Tang, ZH.,Zhao, JL.,Ju, B.,Li, WJ.,Wen, SH.,...&Tang, ZH .(2016).One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum.PROTEIN EXPRESSION AND PURIFICATION,123,70-74.
MLA Tang, ZH,et al."One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum".PROTEIN EXPRESSION AND PURIFICATION 123(2016):70-74.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
One-step chromatogra(559KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Tang, ZH]的文章
[Zhao, JL]的文章
[Ju, B]的文章
百度学术
百度学术中相似的文章
[Tang, ZH]的文章
[Zhao, JL]的文章
[Ju, B]的文章
必应学术
必应学术中相似的文章
[Tang, ZH]的文章
[Zhao, JL]的文章
[Ju, B]的文章
相关权益政策
暂无数据
收藏/分享
文件名: One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。