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题名:
One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum
作者: Tang, ZH; Zhao, JL; Ju, B; Li, WJ; Wen, SH; Pu, Y; Qin, S
刊名: PROTEIN EXPRESSION AND PURIFICATION
ISSN号: 1046-5928
出版日期: 2016-07-01
卷号: 123, 页码:70-74
关键词: Porphyridium cruentum ; B-phycoerythrin ; Purification ; Chromatography
DOI: 10.1016/j.pep.2016.01.018
产权排序: [Tang, Zhihong; Zhao, Jilu; Ju, Bao; Wen, Shaohong] Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China; [Li, Wenjun; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui St, Yantai 264003, Peoples R China; [Pu, Yang] Ludong Univ, Coll Agr, 186 Hongqizhong St, Yantai 264025, Peoples R China
通讯作者: Tang, ZH (reprint author), Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China. zhhtang6@163.com
作者部门: 海岸带生物学与生物资源利用所重点实验室
英文摘要: B-phycoerythrin (B-PE) was separated and purified from microalga Porphyridium cruentum using one-step chromatographic method. Phycobiliproteins in P. cruentum was extracted by osmotic shock and initially purified by ultrafiltration. Further purification was carried out with a SOURCE 15Q exchange column and analytical grade B-PE was obtained with a purity ratio (A(545)/A(280)) of 5.1 and a yield of 68.5%. It showed a double absorption peaks at 545 nm and 565 nm and a shoulder peak at 498 nm, and displayed a fluorescence emission maximum at 580 nm. The analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed a bulky band between 18 and 20 kDa which could be assigned to subunits alpha and beta and a low intensity band of 27 kDa assigned to gamma subunit. Our protocol provides attractive alternative to consider for the purification procedure to obtain analytical grade B-PE at commercial level. (C) 2016 Elsevier Inc. All rights reserved.
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
项目资助者: Ocean Public Welfare Scientific Research Project, State Oceanic Administration, People's Republic of China(20120527)
关键词[WOS]: BED ADSORPTION CHROMATOGRAPHY ; R-PHYCOERYTHRIN ; PHYCOBILIPROTEINS ; SEPARATION ; RECOVERY ; ALLOPHYCOCYANIN ; PHYCOBILISOMES ; PHYCOCYANIN ; EXTRACTION ; PROTEINS
文章类型: Article
收录类别: SCI
语种: 英语
WOS记录号: WOS:000376221600010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/17077
Appears in Collections:中科院海岸带环境过程与生态修复重点实验室_期刊论文

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作者单位: 1.Yantai Univ, Coll Life Sci
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res
3.Ludong Univ

Recommended Citation:
Tang, ZH,Zhao, JL,Ju, B,et al. One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum[J]. PROTEIN EXPRESSION AND PURIFICATION,2016,123:70-74.
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文件名: One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum.pdf
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