One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum

doi:10.1016/j.pep.2016.01.018

	One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum
	Tang, ZH ; Zhao, JL ; Ju, B ; Li, WJ ; Wen, SH ; Pu, Y ; Qin, S; Tang, ZH (reprint author), Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China. zhhtang6@163.com
发表期刊	PROTEIN EXPRESSION AND PURIFICATION
ISSN	1046-5928
	2016-07-01
卷号	123 页码:70-74
关键词	Porphyridium Cruentum B-phycoerythrin Purification Chromatography
DOI	10.1016/j.pep.2016.01.018
产权排序	[Tang, Zhihong; Zhao, Jilu; Ju, Bao; Wen, Shaohong] Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China; [Li, Wenjun; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui St, Yantai 264003, Peoples R China; [Pu, Yang] Ludong Univ, Coll Agr, 186 Hongqizhong St, Yantai 264025, Peoples R China
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	B-phycoerythrin (B-PE) was separated and purified from microalga Porphyridium cruentum using one-step chromatographic method. Phycobiliproteins in P. cruentum was extracted by osmotic shock and initially purified by ultrafiltration. Further purification was carried out with a SOURCE 15Q exchange column and analytical grade B-PE was obtained with a purity ratio (A(545)/A(280)) of 5.1 and a yield of 68.5%. It showed a double absorption peaks at 545 nm and 565 nm and a shoulder peak at 498 nm, and displayed a fluorescence emission maximum at 580 nm. The analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed a bulky band between 18 and 20 kDa which could be assigned to subunits alpha and beta and a low intensity band of 27 kDa assigned to gamma subunit. Our protocol provides attractive alternative to consider for the purification procedure to obtain analytical grade B-PE at commercial level. (C) 2016 Elsevier Inc. All rights reserved.
文章类型	Article
资助机构	Ocean Public Welfare Scientific Research Project, State Oceanic Administration, People's Republic of China(20120527)
收录类别	SCI
语种	英语
关键词[WOS]	BED ADSORPTION CHROMATOGRAPHY ; R-PHYCOERYTHRIN ; PHYCOBILIPROTEINS ; SEPARATION ; RECOVERY ; ALLOPHYCOCYANIN ; PHYCOBILISOMES ; PHYCOCYANIN ; EXTRACTION ; PROTEINS
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000376221600010
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/17077
专题	中国科学院海岸带环境过程与生态修复重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Tang, ZH (reprint author), Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China. zhhtang6@163.com
作者单位	1.Yantai Univ, Coll Life Sci 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res 3.Ludong Univ
推荐引用方式 GB/T 7714	Tang, ZH,Zhao, JL,Ju, B,et al. One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum[J]. PROTEIN EXPRESSION AND PURIFICATION,2016,123:70-74.
APA	Tang, ZH.,Zhao, JL.,Ju, B.,Li, WJ.,Wen, SH.,...&Tang, ZH .(2016).One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum.PROTEIN EXPRESSION AND PURIFICATION,123,70-74.
MLA	Tang, ZH,et al."One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum".PROTEIN EXPRESSION AND PURIFICATION 123(2016):70-74.

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