A Chemotaxis Receptor Modulates Nodulation during the Azorhizobium caulinodans-Sesbania rostrata Symbiosis

doi:10.1128/AEM.00230-16

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	A Chemotaxis Receptor Modulates Nodulation during the Azorhizobium caulinodans-Sesbania rostrata Symbiosis
	Jiang, N ; Liu, W; Li, Y; Wu, HL ; Zhang, ZH ; Alexandre, G ; Elmerich, C ; Xie, ZH ; Xie, ZH (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China. zhxie@yic.ac.cn
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
	2016-06-01
卷号	82 期号:11 页码:3174-3184
DOI	10.1128/AEM.00230-16
产权排序	[Jiang, Nan; Liu, Wei; Li, Yan; Wu, Hailong; Xie, Zhihong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; [Jiang, Nan; Wu, Hailong] Univ Chinese Acad Sci, Sch Resource & Environm, Beijing, Peoples R China; [Zhang, Zhenhai] Beijing Inst Technol, Sch Mechatron Engn, Beijing 100081, Peoples R China; [Alexandre, Gladys] Univ Tennessee, Biochem Cellular & Mol Biol Dept, Knoxville, TN USA; [Elmerich, Claudine] Inst Pasteur, Paris, France
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	Azorhizobium caulinodans ORS571 is a free-living nitrogen-fixing bacterium which can induce nitrogen-fixing nodules both on the root and the stem of its legume host Sesbania rostrata. This bacterium, which is an obligate aerobe that moves by means of a polar flagellum, possesses a single chemotaxis signal transduction pathway. The objective of this work was to examine the role that chemotaxis and aerotaxis play in the lifestyle of the bacterium in free-living and symbiotic conditions. In bacterial chemotaxis, chemoreceptors sense environmental changes and transmit this information to the chemotactic machinery to guide motile bacteria to preferred niches. Here, we characterized a chemoreceptor of A. caulinodans containing an N-terminal PAS domain, named IcpB. IcpB is a soluble heme-binding protein that localized at the cell poles. An icpB mutant strain was impaired in sensing oxygen gradients and in chemotaxis response to organic acids. Compared to the wild-type strain, the icpB mutant strain was also affected in the production of extracellular polysaccharides and impaired in flocculation. When inoculated alone, the icpB mutant induced nodules on S. rostrata, but the nodules formed were smaller and had reduced N-2-fixing activity. The icpB mutant failed to nodulate its host when inoculated competitively with the wild-type strain. Together, the results identify chemotaxis and sensing of oxygen by IcpB as key regulators of the A. caulinodans-S. rostrata symbiosis.
文章类型	Article
资助机构	key research program of the Chinese Academy of Sciences(KZZD-EW-14) ; National Natural Science Foundation of China (NSFC)(31370108 ; National Science Foundation (NSF)(1330344) ; 31570063 ; 60903067)
收录类别	SCI
语种	英语
关键词[WOS]	METHYL-ACCEPTING CHEMOTAXIS ; SIGNAL-TRANSDUCTION ; ESCHERICHIA-COLI ; AZOSPIRILLUM-BRASILENSE ; RHIZOBIUM-LEGUMINOSARUM ; BACTERIAL CHEMORECEPTORS ; GENE-EXPRESSION ; PROTEINS ; HEME ; AEROTAXIS
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000376159400003
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/17076
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Xie, ZH (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China. zhxie@yic.ac.cn
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat 2.Univ Chinese Acad Sci, Sch Resource & Environm 3.Beijing Inst Technol, Sch Mechatron Engn 4.Univ Tennessee, Biochem Cellular & Mol Biol Dept 5.Inst Pasteur
推荐引用方式 GB/T 7714	Jiang, N,Liu, W,Li, Y,et al. A Chemotaxis Receptor Modulates Nodulation during the Azorhizobium caulinodans-Sesbania rostrata Symbiosis[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2016,82(11):3174-3184.
APA	Jiang, N.,Liu, W.,Li, Y.,Wu, HL.,Zhang, ZH.,...&Xie, ZH .(2016).A Chemotaxis Receptor Modulates Nodulation during the Azorhizobium caulinodans-Sesbania rostrata Symbiosis.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,82(11),3174-3184.
MLA	Jiang, N,et al."A Chemotaxis Receptor Modulates Nodulation during the Azorhizobium caulinodans-Sesbania rostrata Symbiosis".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 82.11(2016):3174-3184.

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