Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR
Yu, NN; Ding, JW; Wang, WW; Wang, XD; Qin, W; Ding, JW (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat,YIC, Yantai 264003, Shandong, Peoples R China.; Wang, XD (reprint author), Wenzhou Med Univ, Dept Environm Sci, Key Lab Watershed Sci & Hlth Zhejiang Prov, Wenzhou 325035, Peoples R China. jwding@yic.ac.cn; zjuwxd@163.com
2016-07-01
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
卷号230页码:785-790
产权排序[Yu, Nana; Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat,YIC, Yantai 264003, Shandong, Peoples R China; [Yu, Nana; Wang, Wenwei; Wang, Xuedong] Wenzhou Med Univ, Dept Environm Sci, Key Lab Watershed Sci & Hlth Zhejiang Prov, Wenzhou 325035, Peoples R China
摘要We report here on the development of a chronopotentiometric assay for microcystin-LR based on enzymatic inhibition. The inhibition of protein phosphatase by microcystin-LR can be sensed potentiometrically by using 4-nitrophenyl phosphate as an enzyme substrate. A solid-contact ion-selective electrode (ISE) with poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) as a transduction layer has been designed for potentiometric biosensing using the pulsed galvanastatic technique. By applying an anodic current, the enzymatic generated p-nitrophenol can be extracted into the polymeric membrane with tetradodecylammonium tetrakis(4-chlorophenyl)-borate to produce the chronopotentiometric signal. Meanwhile, a controlled voltage was applied to refresh the membrane for multiple consecutive measurements. The proposed potentiometric assay showed a linear response for microcystin-LR in the range 1-100 mu g/L with a detection limit of 0.5 mu g/L (3 sigma). We believe that the proposed method can be employed for sensitive, rapid and reliable determination of analytes involved in enzyme inhibition. (C) 2016 Elsevier B.V. All rights reserved.
关键词Chronopotentiometry Solid-contact Ion-selective Electrodes Protein Phosphatases Microcystin-lr
作者部门中科院海岸带环境过程与生态修复重点实验室
DOI10.1016/j.snb.2016.02.121
项目资助者National Natural Science Foundation of China(21207156 ; Youth Innovation Promotion Association of CAS(2013139) ; Taishan Scholar Program of Shandong Province ; Zhejiang Provincial Natural Science Foundation(LY14B070013) ; Public Beneficial Project(2015C33306) ; 21575158 ; 41176081)
收录类别SCI
关键词[WOS]LABEL-FREE ; MEMBRANE ELECTRODES ; SENSITIVE DETECTION ; INHIBITION ASSAY ; ELECTROCHEMICAL DETECTION ; PROTEIN PHOSPHATASES ; ENZYME ; WATER ; QUANTIFICATION ; IMMUNOSENSOR
文章类型Article
语种英语
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000374329300100
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/17056
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Ding, JW (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat,YIC, Yantai 264003, Shandong, Peoples R China.; Wang, XD (reprint author), Wenzhou Med Univ, Dept Environm Sci, Key Lab Watershed Sci & Hlth Zhejiang Prov, Wenzhou 325035, Peoples R China. jwding@yic.ac.cn; zjuwxd@163.com
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat,YIC
2.Wenzhou Med Univ, Dept Environm Sci, Key Lab Watershed Sci & Hlth Zhejiang Prov
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Yu, NN,Ding, JW,Wang, WW,et al. Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,230:785-790.
APA Yu, NN.,Ding, JW.,Wang, WW.,Wang, XD.,Qin, W.,...&zjuwxd@163.com.(2016).Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR.SENSORS AND ACTUATORS B-CHEMICAL,230,785-790.
MLA Yu, NN,et al."Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR".SENSORS AND ACTUATORS B-CHEMICAL 230(2016):785-790.
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