Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR | |
Yu, NN; Ding, JW ; Wang, WW; Wang, XD; Qin, W; Ding, JW (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat,YIC, Yantai 264003, Shandong, Peoples R China.; Wang, XD (reprint author), Wenzhou Med Univ, Dept Environm Sci, Key Lab Watershed Sci & Hlth Zhejiang Prov, Wenzhou 325035, Peoples R China. jwding@yic.ac.cn; zjuwxd@163.com
| |
| 发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| ISSN | 0925-4005 |
| 2016-07-01 | |
| 卷号 | 230页码:785-790 |
| 关键词 | Chronopotentiometry Solid-contact Ion-selective Electrodes Protein Phosphatases Microcystin-lr |
| DOI | 10.1016/j.snb.2016.02.121 |
| 产权排序 | [Yu, Nana; Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat,YIC, Yantai 264003, Shandong, Peoples R China; [Yu, Nana; Wang, Wenwei; Wang, Xuedong] Wenzhou Med Univ, Dept Environm Sci, Key Lab Watershed Sci & Hlth Zhejiang Prov, Wenzhou 325035, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | We report here on the development of a chronopotentiometric assay for microcystin-LR based on enzymatic inhibition. The inhibition of protein phosphatase by microcystin-LR can be sensed potentiometrically by using 4-nitrophenyl phosphate as an enzyme substrate. A solid-contact ion-selective electrode (ISE) with poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) as a transduction layer has been designed for potentiometric biosensing using the pulsed galvanastatic technique. By applying an anodic current, the enzymatic generated p-nitrophenol can be extracted into the polymeric membrane with tetradodecylammonium tetrakis(4-chlorophenyl)-borate to produce the chronopotentiometric signal. Meanwhile, a controlled voltage was applied to refresh the membrane for multiple consecutive measurements. The proposed potentiometric assay showed a linear response for microcystin-LR in the range 1-100 mu g/L with a detection limit of 0.5 mu g/L (3 sigma). We believe that the proposed method can be employed for sensitive, rapid and reliable determination of analytes involved in enzyme inhibition. (C) 2016 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China(21207156 ; Youth Innovation Promotion Association of CAS(2013139) ; Taishan Scholar Program of Shandong Province ; Zhejiang Provincial Natural Science Foundation(LY14B070013) ; Public Beneficial Project(2015C33306) ; 21575158 ; 41176081) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | LABEL-FREE ; MEMBRANE ELECTRODES ; SENSITIVE DETECTION ; INHIBITION ASSAY ; ELECTROCHEMICAL DETECTION ; PROTEIN PHOSPHATASES ; ENZYME ; WATER ; QUANTIFICATION ; IMMUNOSENSOR |
| 研究领域[WOS] | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS记录号 | WOS:000374329300100 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/17056 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Ding, JW (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat,YIC, Yantai 264003, Shandong, Peoples R China.; Wang, XD (reprint author), Wenzhou Med Univ, Dept Environm Sci, Key Lab Watershed Sci & Hlth Zhejiang Prov, Wenzhou 325035, Peoples R China. jwding@yic.ac.cn; zjuwxd@163.com |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat,YIC 2.Wenzhou Med Univ, Dept Environm Sci, Key Lab Watershed Sci & Hlth Zhejiang Prov |
| 推荐引用方式 GB/T 7714 | Yu, NN,Ding, JW,Wang, WW,et al. Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,230:785-790. |
| APA | Yu, NN.,Ding, JW.,Wang, WW.,Wang, XD.,Qin, W.,...&zjuwxd@163.com.(2016).Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR.SENSORS AND ACTUATORS B-CHEMICAL,230,785-790. |
| MLA | Yu, NN,et al."Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR".SENSORS AND ACTUATORS B-CHEMICAL 230(2016):785-790. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Pulsed galvanostatic（927KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论