A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles

doi:10.1016/j.ecoenv.2015.05.005

	A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles
	Li, LZ; Wu, HF; Ji, CL; van Gestel, CAM; Allen, HE; Peijnenburg, WJGM; Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc,YICCA, 17,Chunhui Rd, Yantai 264003, Shandong, Peoples R China. hfwu@yic.ac.cn
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
	2015-09
卷号	119 页码:66-73
关键词	Daphnia Magna Silver Nanoparticle Metabolomics Silver Ion Sublethal Toxicity
DOI	10.1016/j.ecoenv.2015.05.005
产权排序	[Li, LianZhen; Wu, Huifeng; Ji, Chenglong] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China; [van Gestel, Cornelis A. M.] Vrije Univ Amsterdam, Fac Earth & Life Sci, Dept Ecol Sci, NL-1081 HV Amsterdam, Netherlands; [Allen, Herbert E.] Univ Delaware, Dept Civil & Environm Engn, Newark, DE 19716 USA; [Peijnenburg, Willie J. G. M.] Natl Inst Publ Hlth & Environm, Ctr Safety Subst & Prod, NL-3720 BA Bilthoven, Netherlands; [Peijnenburg, Willie J. G. M.] Leiden Univ, Inst Environm Sci CML, Leiden, Netherlands
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	We examined the short-term toxicity of AgNPs and AgNO3 to Daphnia magna at sublethal levels using H-1 NMR-based metabolomics. Two sizes of polyvinylpyrrolidone-coated AgNPs (10 and 40 nm) were synthesized and characterized and their Ag+ release was studied using centrifugal ultrafiltration and inductively coupled plasma mass spectrometry. Multivariate statistical analysis of the H-1 NMR spectra showed significant changes in the D. magna metabolic profiles following 48 h exposure to both AgNP particle sizes and Ag+ exposure. Most of the metabolic biomarkers for AgNP exposure, including 3-hydroxybutyrate, arginine, lysine and phosphocholine, were identical to those of the Ag+-exposed groups, suggesting that the dominant effects of both AgNPs were due to released Ag+. The observed metabolic changes implied that the released Ag+ induced disturbance in energy metabolism and oxidative stress, a proposed mechanism of AgNP toxicity. Elevated levels of lactate in all AgNP-treated but not in Ag+-treated groups provided evidence for Ag-NP enhanced anaerobic metabolism. These findings show that H-1 NMR-based metabolomics provides a sensitive measure of D. magna response to AgNPs and that further targeted assays are needed to elucidate mechanisms of action of nanoparticle-induced toxicity. (C) 2015 Elsevier Inc. All rights reserved.
文章类型	期刊论文
收录类别	SCI
语种	英语
关键词[WOS]	TOXICITY ; NMR ; EXTRACTION ; ORGANISMS ; STRESS ; ACID ; RATS
研究领域[WOS]	Environmental Sciences ; Toxicology
WOS记录号	WOS:000356841200008
引用统计	被引频次：47[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/13813
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc,YICCA, 17,Chunhui Rd, Yantai 264003, Shandong, Peoples R China. hfwu@yic.ac.cn
推荐引用方式 GB/T 7714	Li, LZ,Wu, HF,Ji, CL,et al. A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2015,119:66-73.
APA	Li, LZ.,Wu, HF.,Ji, CL.,van Gestel, CAM.,Allen, HE.,...&Wu, HF .(2015).A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,119,66-73.
MLA	Li, LZ,et al."A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 119(2015):66-73.

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