YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
A near-infrared ratiometric fluorescent probe for cysteine detection over glutathione indicating mitochondrial oxidative stress in vivo
Yin, Kun1,2,3; Yu, Fabiao1,2; Zhang, Weiwei1,2,4; Chen, Lingxin1,2; Yu, FB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. fbyu@yic.ac.cn; lxchen@yic.ac.cn
2015-12-15
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume74Pages:156-164
Contribution Rank[Yin, Kun; Yu, Fabiao; Zhang, Weiwei; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Yin, Kun; Yu, Fabiao; Zhang, Weiwei; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China; [Yin, Kun] Univ Chinese Acad Sci, Being 100049, Peoples R China; [Zhang, Weiwei] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
AbstractWe establish a near-infrared (NIR) ratiometric fluorescent probe Cy-NB for the selective detection of cysteine (Cys) over glutathione (GSH) and homocysteine (Hcy) in mitochondria to indicate oxidative stress. Heptamethine cyanine dye is chosen as the fluorophore of Cy-NB whose emission locates in NIR region. And p-nitrobenzoyl is employed as the fluorescent modulator due to its capability of selective-Cys response. Once triggered by Cys, the uncaged p-nitrobenzoyl rearranges the polymethine pi-electron system of the fluorophore, which leads to a remarkable spectrum shifts in absorption and emission profiles. Taking advantage of these spectroscopic properties, we construct a ratiometric fluorescent signal for the detection of Cys with a detection limit of 0.2 mu M within 5 min. Our probe Cy-NB can sensitively detect the mitochondrial Cys pool changes under different oxidative stress status in HepG2 cells. We also successfully employ Cy-NB to imaging Cys level changes in living mice. It suggests that mitochondrial Cys can be used as an oxidative stress biomarker with simple potential clinical applications. And our probe Cy-NB is of great potential for further utilizing in exploring the physiological function of Cys in biological systems. (C) 2015 Elsevier B.V. All rights reserved.; We establish a near-infrared (NIR) ratiometric fluorescent probe Cy-NB for the selective detection of cysteine (Cys) over glutathione (GSH) and homocysteine (Hcy) in mitochondria to indicate oxidative stress. Heptamethine cyanine dye is chosen as the fluorophore of Cy-NB whose emission locates in NIR region. And p-nitrobenzoyl is employed as the fluorescent modulator due to its capability of selective-Cys response. Once triggered by Cys, the uncaged p-nitrobenzoyl rearranges the polymethine pi-electron system of the fluorophore, which leads to a remarkable spectrum shifts in absorption and emission profiles. Taking advantage of these spectroscopic properties, we construct a ratiometric fluorescent signal for the detection of Cys with a detection limit of 0.2 mu M within 5 min. Our probe Cy-NB can sensitively detect the mitochondrial Cys pool changes under different oxidative stress status in HepG2 cells. We also successfully employ Cy-NB to imaging Cys level changes in living mice. It suggests that mitochondrial Cys can be used as an oxidative stress biomarker with simple potential clinical applications. And our probe Cy-NB is of great potential for further utilizing in exploring the physiological function of Cys in biological systems. (C) 2015 Elsevier B.V. All rights reserved.
KeywordCysteine Ratiometric Fluorescence Image Analysis Mitochondria Oxidative Stress
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
DOI10.1016/j.bios.2015.06.039
Funding OrganizationNorth China Sea Branch of State Oceanic Administration(QDZC20150420-002) ; National Natural Science Foundation of China(31200041 ; CAS(KZCX2-EW-206) ; Key Research Program of the CAS(KZZD-EW-14) ; Grant Sponsor Youth Innovation Promotion Association(2015170) ; 21405172 ; 21275158)
Indexed BySCI
WOS KeywordLARGE EMISSION SHIFT ; LIVING CELLS ; HYDROGEN-SULFIDE ; HOMOCYSTEINE ; THIOLS ; DERIVATIZATION ; SENSOR ; ACID ; RATS ; ROS
SubtypeArticle
Language英语
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS IDWOS:000360772800021
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/13808
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorYu, FB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. fbyu@yic.ac.cn; lxchen@yic.ac.cn
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China
3.Univ Chinese Acad Sci, Being 100049, Peoples R China
4.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yin, Kun,Yu, Fabiao,Zhang, Weiwei,et al. A near-infrared ratiometric fluorescent probe for cysteine detection over glutathione indicating mitochondrial oxidative stress in vivo[J]. BIOSENSORS & BIOELECTRONICS,2015,74:156-164.
APA Yin, Kun,Yu, Fabiao,Zhang, Weiwei,Chen, Lingxin,Yu, FB ,&lxchen@yic.ac.cn.(2015).A near-infrared ratiometric fluorescent probe for cysteine detection over glutathione indicating mitochondrial oxidative stress in vivo.BIOSENSORS & BIOELECTRONICS,74,156-164.
MLA Yin, Kun,et al."A near-infrared ratiometric fluorescent probe for cysteine detection over glutathione indicating mitochondrial oxidative stress in vivo".BIOSENSORS & BIOELECTRONICS 74(2015):156-164.
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