Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| A near-infrared ratiometric fluorescent probe for cysteine detection over glutathione indicating mitochondrial oxidative stress in vivo | |
Yin, Kun1,2,3; Yu, Fabiao1,2; Zhang, Weiwei1,2,4; Chen, Lingxin1,2 ; Yu, FB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. fbyu@yic.ac.cn; lxchen@yic.ac.cn
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| 发表期刊 | BIOSENSORS & BIOELECTRONICS
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| ISSN | 0956-5663 |
| 2015-12-15 | |
| 卷号 | 74页码:156-164 |
| 关键词 | Cysteine Ratiometric Fluorescence Image Analysis Mitochondria Oxidative Stress |
| DOI | 10.1016/j.bios.2015.06.039 |
| 产权排序 | [Yin, Kun; Yu, Fabiao; Zhang, Weiwei; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Yin, Kun; Yu, Fabiao; Zhang, Weiwei; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China; [Yin, Kun] Univ Chinese Acad Sci, Being 100049, Peoples R China; [Zhang, Weiwei] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | We establish a near-infrared (NIR) ratiometric fluorescent probe Cy-NB for the selective detection of cysteine (Cys) over glutathione (GSH) and homocysteine (Hcy) in mitochondria to indicate oxidative stress. Heptamethine cyanine dye is chosen as the fluorophore of Cy-NB whose emission locates in NIR region. And p-nitrobenzoyl is employed as the fluorescent modulator due to its capability of selective-Cys response. Once triggered by Cys, the uncaged p-nitrobenzoyl rearranges the polymethine pi-electron system of the fluorophore, which leads to a remarkable spectrum shifts in absorption and emission profiles. Taking advantage of these spectroscopic properties, we construct a ratiometric fluorescent signal for the detection of Cys with a detection limit of 0.2 mu M within 5 min. Our probe Cy-NB can sensitively detect the mitochondrial Cys pool changes under different oxidative stress status in HepG2 cells. We also successfully employ Cy-NB to imaging Cys level changes in living mice. It suggests that mitochondrial Cys can be used as an oxidative stress biomarker with simple potential clinical applications. And our probe Cy-NB is of great potential for further utilizing in exploring the physiological function of Cys in biological systems. (C) 2015 Elsevier B.V. All rights reserved.; We establish a near-infrared (NIR) ratiometric fluorescent probe Cy-NB for the selective detection of cysteine (Cys) over glutathione (GSH) and homocysteine (Hcy) in mitochondria to indicate oxidative stress. Heptamethine cyanine dye is chosen as the fluorophore of Cy-NB whose emission locates in NIR region. And p-nitrobenzoyl is employed as the fluorescent modulator due to its capability of selective-Cys response. Once triggered by Cys, the uncaged p-nitrobenzoyl rearranges the polymethine pi-electron system of the fluorophore, which leads to a remarkable spectrum shifts in absorption and emission profiles. Taking advantage of these spectroscopic properties, we construct a ratiometric fluorescent signal for the detection of Cys with a detection limit of 0.2 mu M within 5 min. Our probe Cy-NB can sensitively detect the mitochondrial Cys pool changes under different oxidative stress status in HepG2 cells. We also successfully employ Cy-NB to imaging Cys level changes in living mice. It suggests that mitochondrial Cys can be used as an oxidative stress biomarker with simple potential clinical applications. And our probe Cy-NB is of great potential for further utilizing in exploring the physiological function of Cys in biological systems. (C) 2015 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | North China Sea Branch of State Oceanic Administration(QDZC20150420-002) ; National Natural Science Foundation of China(31200041 ; CAS(KZCX2-EW-206) ; Key Research Program of the CAS(KZZD-EW-14) ; Grant Sponsor Youth Innovation Promotion Association(2015170) ; 21405172 ; 21275158) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | LARGE EMISSION SHIFT ; LIVING CELLS ; HYDROGEN-SULFIDE ; HOMOCYSTEINE ; THIOLS ; DERIVATIZATION ; SENSOR ; ACID ; RATS ; ROS |
| 研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS记录号 | WOS:000360772800021 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/13808 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Yu, FB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. fbyu@yic.ac.cn; lxchen@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Being 100049, Peoples R China 4.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Kun,Yu, Fabiao,Zhang, Weiwei,et al. A near-infrared ratiometric fluorescent probe for cysteine detection over glutathione indicating mitochondrial oxidative stress in vivo[J]. BIOSENSORS & BIOELECTRONICS,2015,74:156-164. |
| APA | Yin, Kun,Yu, Fabiao,Zhang, Weiwei,Chen, Lingxin,Yu, FB ,&lxchen@yic.ac.cn.(2015).A near-infrared ratiometric fluorescent probe for cysteine detection over glutathione indicating mitochondrial oxidative stress in vivo.BIOSENSORS & BIOELECTRONICS,74,156-164. |
| MLA | Yin, Kun,et al."A near-infrared ratiometric fluorescent probe for cysteine detection over glutathione indicating mitochondrial oxidative stress in vivo".BIOSENSORS & BIOELECTRONICS 74(2015):156-164. |
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