Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp strain HC8-3S

doi:10.1016/j.ibiod.2015.11.007

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	Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp strain HC8-3S
	Liu, YH ; Hu, XK; Liu, H; Hu, XK (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Beijing 100864, Peoples R China. xkhu@yic.ac.cn
发表期刊	INTERNATIONAL BIODETERIORATION & BIODEGRADATION
ISSN	0964-8305
	2016-02-01
卷号	107 页码:56-61
关键词	Acinetobacter Sp. Industrial-scale Fermentation Long-term Storage Crude Oil-degrading Bacterium
DOI	10.1016/j.ibiod.2015.11.007
产权排序	[Liu, Yuhua; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Beijing 100864, Peoples R China; [Liu, Yuhua] Univ Chinese Acad Sci, Beijing, Peoples R China; [Liu, Hui] Diyuan Biol Technol Co Ltd, Yantai, Peoples R China
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	The marine bacterium Acinetobacter sp. strain HC8-3S, capable of degrading petroleum hydrocarbons, was previously shown to be applicable for bioremediation. Here, we evaluated the performance of the strain during industrial-scale fermentation, long-term storage, and biodegradation processes. Fermentation parameters were optimized for industrial-scale culturing using lower salinity and fewer inorganic salts in the culture medium. The shelf-life of the fermentation broth was evaluated in different storage conditions. Biodegradation efficiency of the strain was evaluated using gas chromatography. Results indicated that the optimum conditions for one-ton scale fermentation were 30 degrees C, pH 7.4-7.8, and rotation speed 90 rpm; the cell density reached 3.6 +/- 1.9 x 10(10) CFU ml(-1) after 12 h. The low temperature can preserved fermentation broth for longer time. When the storage temperature was down to 4 degrees C from room temperature, half-life of strain HC8-3S extended from 9 days to 34 days. The biodegradation rate of the saturated hydrocarbon fraction of crude oil was 94% after treatment with the strain for 5 days. The results indicate that Acinetobacter sp. strain HC8-3S can be enriched efficiently on a large scale, making it a potentially useful industrial strain. This crude oil degradative capability of the strain HC8-3S provides possible application for the clean-up of crude oil-contaminated environment. (C) 2015 Elsevier Ltd. All rights reserved.
文章类型	Article
资助机构	National Natural Science Foundation of China(41376138) ; Chinese Academy of Sciences(1191100502)
收录类别	SCI
语种	英语
关键词[WOS]	BACTERIAL COMMUNITY RESPONSE ; PETROLEUM-HYDROCARBONS ; FRESH-WATER ; BIODEGRADATION ; BIOREMEDIATION ; SEA ; DEGRADATION ; POLLUTANTS ; SEDIMENT ; GULF
研究领域[WOS]	Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology
WOS记录号	WOS:000369206700008
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/10878
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Hu, XK (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Beijing 100864, Peoples R China. xkhu@yic.ac.cn
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat 2.Univ Chinese Acad Sci 3.Diyuan Biol Technol Co Ltd
推荐引用方式 GB/T 7714	Liu, YH,Hu, XK,Liu, H,et al. Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp strain HC8-3S[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2016,107:56-61.
APA	Liu, YH,Hu, XK,Liu, H,&Hu, XK .(2016).Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp strain HC8-3S.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,107,56-61.
MLA	Liu, YH,et al."Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp strain HC8-3S".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 107(2016):56-61.

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