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Dual-emission color-controllable nanoparticle based molecular imprinting ratiometric fluorescence sensor for the visual detection of Brilliant Blue 期刊论文
SENSORS AND ACTUATORS B-CHEMICAL, 2019, 卷号: 284, 页码: 428-436
作者:  Yang, Q;  Li, JH;  Wang, XY;  Peng, HL;  Xiong, H;  Chen, LX
浏览  |  Adobe PDF(2081Kb)  |  收藏  |  浏览/下载：508/267  |  提交时间：2020/07/08
Molecular imprinting  Ratiometric fluorescence  Dual-emission nanoparticles  Brilliant Blue  Visual detection  
Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide 期刊论文
MICRO & NANO LETTERS, 2019, 卷号: 14, 期号: 3, 页码: 344-348
作者:  Li, WJ;  Jiang, TT;  Pu, Y;  Jiao, XD;  Tan, WQ;  Qin, S
Adobe PDF(1618Kb)  |  收藏  |  浏览/下载：371/92  |  提交时间：2020/07/08
biochemistry  biosensors  nanosensors  nanofabrication  fluorescence  molecular biophysics  radiation quenching  chemical sensors  sugar  proteins  biomedical optical imaging  graphene compounds  GO-CS  low concentrations  competitive binding  maltose-binding protein  recombinant phycocyanin  far-red fluorescence  MBP-rPC  rPC emission  rPC fluorescence  linear detection range  glucose biosensor  fluorescence quenching  chitosan-modified graphene oxide  low-molecular-weight chitosan  glucose detection  fluorescence resonance energy transfer  graphene oxide nanomaterial  mass 0  1 mg to 1  0 mg  CO  
Mitochondria-targeting near-infrared ratiometric fluorescent probe for selective imaging of cysteine in orthotopic lung cancer mice 期刊论文
SENSORS AND ACTUATORS B-CHEMICAL, 2019, 卷号: 282, 页码: 69-77
作者:  Zhang, Xiaoyu;  He, Na;  Huang, Yan;  Yu, Fabiao;  Li, Bowei;  Lv, Changjun;  Chen, Lingxin
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Fluorescent probes  Near-infrared fluorescence  Cysteine  Mitochondria target  Lung cancer  
食用蔬菜能吸收和积累微塑料 期刊论文
科学通报, 2019, 卷号: 64, 期号: 9, 页码: 928-934
作者:  李连祯;  周倩;  尹娜;  涂晨;  骆永明
收藏  |  浏览/下载：499/0  |  提交时间：2020/06/17
微塑料  生菜  聚苯乙烯微球  吸收  积累  健康风险  microplastics  lettuce  polystyrene microbeads  uptake  accumulation  human health risk  Microplastic (MP, 100 nm-5 mm) may present an attributable risk to ecosystem and human health, and its pollution has become a global environmental concern. Despite a wealth of information on the accumulation of MPs in aquatic species, there is no information on the uptake and accumulation of MPs by higher plants. Terrestrial edible plants are directly exposed to MPs when agricultural soil was applied with organic manure, sewage sludge as fertilizer or plastic mulching. In this paper, the uptake of two sizes of polystyrene (PS) microbeads (0.2 and 1.0 mum) and then their distribution and migration in an edible plant lettuce were firstly investigated based on laboratory experiments. We used fluorescent markers to track PS microbeads in plant tissues and found fluorescence to be a sensitive and reliable detection method. Sections from untreated control lettuce showed no autofluorescence. When roots were treated with fluorescently labeled PS microbeads, the microbeads could be identified by its fluorescence. Our main study investigated the uptake of 0.2 mum beads, as few luminescence signals were observed in lettuce roots for 1.0 mum beads in our experiment. We observed that 0.2 mum fluorescent microbeads were extracellularly trapped in the root cap mucilage (which is a highly hydrated polysaccharide) and a dark green tip (which was typical of lettuce roots exposed to label PS beads) was usually visible to the naked eye. Confocal images revealed that the PS luminescence signals were mainly located in the vascular system and on the cell walls of the cortex tissue of the roots, indicated that the beads passed through the intercellular space via the apoplastic transport system. Once inside the central cylinder, the 0.2 mum PS beads were transferred from the roots to the stems and leaves via the vascular system following the transpiration stream. We also observed that the PS beads adhered to one another and self-assembled systematically into grape-like and (chain) string-like clusters in the intercellular space of the root and stem vascular tissue of lettuce plant. In contrast to the root and stem, PS beads were dispersed in the leaf tissue. Here, for the first time we provide evidence of the adherence, uptake, accumulation, and translocation of submicrometer MPs within an edible plant. Our findings highlight the previously underappreciated human exposure pathway to MPs through the consumption of contaminated crops and emphasize the need for new management strategies to control the release of MPs waste products into the terrestrial environment. Ultimately, the potential impacts of low range sized MPs on food safety of crop plants and human health need to be urgently considered.  
Hybrid coupling of R-phycoerythrin and the orange carotenoid protein supports the FRET-based mechanism of cyanobacterial photoprotection 期刊论文
Biochemical and Biophysical Research Communications, 2019, 卷号: 516, 期号: 3, 页码: 699-704
作者:  Maksimov, EG;  Li WJ(李文军);  Protasova, EA;  Friedrich, T;  Ge, B;  Qin, S;  Sluchanko, NN
Adobe PDF(1177Kb)  |  收藏  |  浏览/下载：355/104  |  提交时间：2020/07/21
Orange Carotenoid Protein  Phycobilisome  Phycobiliproteins  Fluorescence  Photoprotection  Non-photochemical Quenching