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表面增强拉曼散射探针标记纳米塑料在菲律宾蛤仔（Ruditapes philippinarum）体内行为研究 学位论文
硕士, 中国科学院烟台海岸带研究所: 中国科学院大学, 2022
作者:  杜泓钰
Adobe PDF(10861Kb)  |  收藏  |  浏览/下载：128/0  |  提交时间：2024/03/20
表面增强拉曼散射  纳米塑料  聚苯乙烯  菲律宾蛤仔  原位检测  Surface enhanced Raman scattering  Nanoplastics  Polystyrene  Ruditapes philippinarum  In vivo distribution  
微塑料和芘对菲律宾蛤仔的毒性效应研究 期刊论文
海洋通报, 2021, 卷号: 40, 期号: 6, 页码: 644-656
作者:  柳佳佳;  朱效鹏;  滕佳;  赵建民;  李成华;  单恩翠;  张晨;  王清
收藏  |  浏览/下载：82/0  |  提交时间：2024/01/26
菲律宾蛤仔  聚苯乙烯微塑料  芘  生理响应  氧化应激  免疫防御  
禾本科作物小麦能吸收和积累聚苯乙烯塑料微球 期刊论文
科学通报, 2020, 卷号: 65.0, 期号: 020, 页码: 2120-2127
作者:  李瑞杰;  李连祯;  张云超;  杨杰;  涂晨;  周倩;  李远;  骆永明
浏览  |  Adobe PDF(2104Kb)  |  收藏  |  浏览/下载：615/229  |  提交时间：2021/06/16
小麦幼苗  聚苯乙烯微球  砂培  吸收  积累  
禾本科作物小麦能吸收和积累聚苯乙烯塑料微球 期刊论文
科学通报, 2020, 卷号: 65, 期号: 20, 页码: 2120-2127
作者:  李瑞杰;  李连祯;  张云超;  杨杰;  涂晨;  周倩;  李远;  骆永明
浏览  |  Adobe PDF(2104Kb)  |  收藏  |  浏览/下载：515/203  |  提交时间：2021/12/01
小麦幼苗  聚苯乙烯微球  砂培  吸收  积累  
食用蔬菜能吸收和积累微塑料 期刊论文
科学通报, 2019, 卷号: 64, 期号: 9, 页码: 928-934
作者:  李连祯;  周倩;  尹娜;  涂晨;  骆永明
收藏  |  浏览/下载：536/0  |  提交时间：2020/06/17
微塑料  生菜  聚苯乙烯微球  吸收  积累  健康风险  microplastics  lettuce  polystyrene microbeads  uptake  accumulation  human health risk  Microplastic (MP, 100 nm-5 mm) may present an attributable risk to ecosystem and human health, and its pollution has become a global environmental concern. Despite a wealth of information on the accumulation of MPs in aquatic species, there is no information on the uptake and accumulation of MPs by higher plants. Terrestrial edible plants are directly exposed to MPs when agricultural soil was applied with organic manure, sewage sludge as fertilizer or plastic mulching. In this paper, the uptake of two sizes of polystyrene (PS) microbeads (0.2 and 1.0 mum) and then their distribution and migration in an edible plant lettuce were firstly investigated based on laboratory experiments. We used fluorescent markers to track PS microbeads in plant tissues and found fluorescence to be a sensitive and reliable detection method. Sections from untreated control lettuce showed no autofluorescence. When roots were treated with fluorescently labeled PS microbeads, the microbeads could be identified by its fluorescence. Our main study investigated the uptake of 0.2 mum beads, as few luminescence signals were observed in lettuce roots for 1.0 mum beads in our experiment. We observed that 0.2 mum fluorescent microbeads were extracellularly trapped in the root cap mucilage (which is a highly hydrated polysaccharide) and a dark green tip (which was typical of lettuce roots exposed to label PS beads) was usually visible to the naked eye. Confocal images revealed that the PS luminescence signals were mainly located in the vascular system and on the cell walls of the cortex tissue of the roots, indicated that the beads passed through the intercellular space via the apoplastic transport system. Once inside the central cylinder, the 0.2 mum PS beads were transferred from the roots to the stems and leaves via the vascular system following the transpiration stream. We also observed that the PS beads adhered to one another and self-assembled systematically into grape-like and (chain) string-like clusters in the intercellular space of the root and stem vascular tissue of lettuce plant. In contrast to the root and stem, PS beads were dispersed in the leaf tissue. Here, for the first time we provide evidence of the adherence, uptake, accumulation, and translocation of submicrometer MPs within an edible plant. Our findings highlight the previously underappreciated human exposure pathway to MPs through the consumption of contaminated crops and emphasize the need for new management strategies to control the release of MPs waste products into the terrestrial environment. Ultimately, the potential impacts of low range sized MPs on food safety of crop plants and human health need to be urgently considered.  
海岸环境中两类软质微塑料表面生物膜DNA的提取方法比较与优化 期刊论文
海洋学报, 2018, 卷号: 40, 期号: 12, 页码: 112-119
作者:  陈涛;  涂晨;  郭鹏鹏;  周倩;  骆永明
收藏  |  浏览/下载：362/0  |  提交时间：2020/07/07
微塑料  生物膜  DNA  聚乙烯薄膜(PE)  发泡类聚苯乙烯(EPS)