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藻类特有的捕光色素蛋白——藻红蛋白的结构、功能及应用 期刊论文
科学通报, 2020, 卷号: 65, 期号: 07, 页码: 565-576
作者:  臧帆;  秦松;  马丞博;  李文军;  林剑
Adobe PDF(1397Kb)  |  收藏  |  浏览/下载：768/241  |  提交时间：2020/07/08
藻红蛋白  结构功能  制备工艺  光学活性  生物活性  
环境友好型比率荧光微流控纸芯片快速检测苯醚甲环唑 期刊论文
中国科学:化学, 2020, 卷号: 50, 期号: 03, 页码: 393-405
作者:  马昕;  郝帼英;  张忠;  李金花;  杨兴斌;  陈令新
Adobe PDF(13626Kb)  |  收藏  |  浏览/下载：540/213  |  提交时间：2020/07/08
分子印迹  碳量子点  比率荧光  苯醚甲环唑  微流控纸芯片  
藻类特有的捕光色素蛋白藻红蛋白的结构、功能及应用 期刊论文
科学通报, 2020, 卷号: 65, 期号: 7, 页码: 565-576
作者:  臧帆;  秦松;  马丞博;  李文军;  林剑
Adobe PDF(1396Kb)  |  收藏  |  浏览/下载：198/51  |  提交时间：2021/12/01
藻红蛋白  结构功能  制备工艺  光学活性  生物活性  
禾本科作物小麦能吸收和积累聚苯乙烯塑料微球 期刊论文
科学通报, 2020, 卷号: 65.0, 期号: 020, 页码: 2120-2127
作者:  李瑞杰;  李连祯;  张云超;  杨杰;  涂晨;  周倩;  李远;  骆永明
Adobe PDF(2104Kb)  |  收藏  |  浏览/下载：592/221  |  提交时间：2021/06/16
小麦幼苗  聚苯乙烯微球  砂培  吸收  积累  
禾本科作物小麦能吸收和积累聚苯乙烯塑料微球 期刊论文
科学通报, 2020, 卷号: 65, 期号: 20, 页码: 2120-2127
作者:  李瑞杰;  李连祯;  张云超;  杨杰;  涂晨;  周倩;  李远;  骆永明
Adobe PDF(2104Kb)  |  收藏  |  浏览/下载：501/199  |  提交时间：2021/12/01
小麦幼苗  聚苯乙烯微球  砂培  吸收  积累  
环境友好型比率荧光微流控纸芯片快速检测苯醚甲环唑 期刊论文
中国科学. 化学, 2020, 卷号: 50, 期号: 3, 页码: 393-405
作者:  马昕;  郝帼英;  张忠;  李金花;  杨兴斌;  陈令新
浏览  |  Adobe PDF(13626Kb)  |  收藏  |  浏览/下载：390/148  |  提交时间：2021/12/01
分子印迹  碳量子点  比率荧光  苯醚甲环唑  微流控纸芯片  
食用蔬菜能吸收和积累微塑料 期刊论文
科学通报, 2019, 卷号: 64, 期号: 9, 页码: 928-934
作者:  李连祯;  周倩;  尹娜;  涂晨;  骆永明
收藏  |  浏览/下载：529/0  |  提交时间：2020/06/17
微塑料  生菜  聚苯乙烯微球  吸收  积累  健康风险  microplastics  lettuce  polystyrene microbeads  uptake  accumulation  human health risk  Microplastic (MP, 100 nm-5 mm) may present an attributable risk to ecosystem and human health, and its pollution has become a global environmental concern. Despite a wealth of information on the accumulation of MPs in aquatic species, there is no information on the uptake and accumulation of MPs by higher plants. Terrestrial edible plants are directly exposed to MPs when agricultural soil was applied with organic manure, sewage sludge as fertilizer or plastic mulching. In this paper, the uptake of two sizes of polystyrene (PS) microbeads (0.2 and 1.0 mum) and then their distribution and migration in an edible plant lettuce were firstly investigated based on laboratory experiments. We used fluorescent markers to track PS microbeads in plant tissues and found fluorescence to be a sensitive and reliable detection method. Sections from untreated control lettuce showed no autofluorescence. When roots were treated with fluorescently labeled PS microbeads, the microbeads could be identified by its fluorescence. Our main study investigated the uptake of 0.2 mum beads, as few luminescence signals were observed in lettuce roots for 1.0 mum beads in our experiment. We observed that 0.2 mum fluorescent microbeads were extracellularly trapped in the root cap mucilage (which is a highly hydrated polysaccharide) and a dark green tip (which was typical of lettuce roots exposed to label PS beads) was usually visible to the naked eye. Confocal images revealed that the PS luminescence signals were mainly located in the vascular system and on the cell walls of the cortex tissue of the roots, indicated that the beads passed through the intercellular space via the apoplastic transport system. Once inside the central cylinder, the 0.2 mum PS beads were transferred from the roots to the stems and leaves via the vascular system following the transpiration stream. We also observed that the PS beads adhered to one another and self-assembled systematically into grape-like and (chain) string-like clusters in the intercellular space of the root and stem vascular tissue of lettuce plant. In contrast to the root and stem, PS beads were dispersed in the leaf tissue. Here, for the first time we provide evidence of the adherence, uptake, accumulation, and translocation of submicrometer MPs within an edible plant. Our findings highlight the previously underappreciated human exposure pathway to MPs through the consumption of contaminated crops and emphasize the need for new management strategies to control the release of MPs waste products into the terrestrial environment. Ultimately, the potential impacts of low range sized MPs on food safety of crop plants and human health need to be urgently considered.  
用于检测细胞中亚硝酰氢的近红外荧光探针及其生物应用 期刊论文
分析化学, 2015, 卷号: 43, 期号: 12, 页码: 1829-1836
作者:  刘萍;  韩潇玥;  于法标;  陈令新;  于法标,中国科学院海岸带环境过程与生态修复重点实验室,E-mail: fbyu@yic.ac.cn;  陈令新,中国科学院海岸带环境过程与生态修复重点实验室,E-mail: lxchen@yic.ac.cn
浏览  |  Adobe PDF(2385Kb)  |  收藏  |  浏览/下载：737/305  |  提交时间：2016/12/15
荧光探针  亚硝酰氢  细胞分析  近红外成像