| A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress | |
| Zhang, DY; Tong, JF; He, XL; Xu, ZL; Xu, L; Wei, PP; Huang, YH; Brestic, M; Ma, HX; Shao, HB; Zhang, DY (reprint author), Jiangsu Acad Agr Sci, Inst Agrobiotechnol, Prov Key Lab Agrobiol, Jiangsu Key Lab Bioresources Saline Soils, Nanjing, Jiangsu, Peoples R China. cotton.z@126.com; shaohongbochu@126.com | |
| 发表期刊 | FRONTIERS IN PLANT SCIENCE
 |
| ISSN | 1664-462X |
| 2016-01-08 | |
| 卷号 | 6 |
| 关键词 | Soybean Gmtip2 Heterologous Expression Promoter Osmotic Stress 3 |
| DOI | 10.3389/fpls.2015.01237 |
| 产权排序 | [Zhang, Dayong; He, Xiaolan; Xu, Zhaolong; Xu, Ling; Wei, Peipei; Huang, Yihong; Brestic, Marian; Ma, Hongxiang; Shao, Hongbo] Jiangsu Acad Agr Sci, Inst Agrobiotechnol, Prov Key Lab Agrobiol, Jiangsu Key Lab Bioresources Saline Soils, Nanjing, Jiangsu, Peoples R China; [Tong, Jinfeng] Jiangsu Prov & Chinese Acad Sci, Inst Bot, Nanjing, Jiangsu, Peoples R China; [Brestic, Marian] Slovak Univ Agr, Dept Plant Physiol, Nitra, Slovakia; [Shao, Hongbo] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Water is essential for plant growth and development. Water deficiency leads to loss of yield and decreased crop quality. To understand water transport mechanisms in plants, we cloned and characterized a novel tonoplast intrinsic protein (TIP) gene from soybean with the highest similarity to TIP2-type from other plants, and thus designated GmTIP2;3. The protein sequence contains two conserved NPA motifs and six transmembrane domains. The expression analysis indicated that this gene was constitutively expressed in all detected tissues, with higher levels in the root, stem and pod, and the accumulation of GmTIP2;3 transcript showed a significant response to osmotic stresses, including 20% PEG6000 (polyethylene glycol) and 100 mu M ABA (abscisic acid) treatments. The promoter-GUS (glucuronidase) activity analysis suggested that GmTIP2;3 was also expressed in the root, stem, and leaf, and preferentially expressed in the stele of root and stem, and the core promoter region was 1000 bp in length, located upstream of the ATG start codon. The GUS tissue and induced expression observations were consistent with the findings in soybean. In addition, subcellular localization showed that GmTIP2;3 was a plasma membrane localized protein. Yeast heterologous expression revealed that Gm TIP2;3 could improve tolerance to osmotic stress in yeast cells. Integrating these results, Gm TIP2;3 might play an important role in response to osmotic stress in plants. |
| 文章类型 | Article |
| 资助机构 | National Science Foundation of China(31101166) ; Jiangsu Natural Science Foundation, China(BK20151364) ; Open Foundation of the Jiangsu Key Laboratory for Bioresources of Saline Soils(JKLBS2014002) ; One Hundred Talent Plan of Foreign Experts of Jiangsu Province(JSB2015005) ; Project in the National Science and Technology Pillar Program during the Twelfth Five-year Plan Period(2011BAD35B06-4-3) ; Jiangsu Autonomous Innovation of Agricultural Science and Technology(CX(15)1005) |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PLASMA-MEMBRANE AQUAPORINS ; TRANSGENIC ARABIDOPSIS ; DROUGHT TOLERANCE ; HIGHER-PLANTS ; EXPRESSION ; WATER ; IDENTIFICATION ; GENOME ; SALT ; TRANSFORMATION |
| 研究领域[WOS] | Plant Sciences |
| WOS记录号 | WOS:000367735300001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/9295 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Zhang, DY (reprint author), Jiangsu Acad Agr Sci, Inst Agrobiotechnol, Prov Key Lab Agrobiol, Jiangsu Key Lab Bioresources Saline Soils, Nanjing, Jiangsu, Peoples R China. cotton.z@126.com; shaohongbochu@126.com |
| 作者单位 | 1.Jiangsu Acad Agr Sci, Inst Agrobiotechnol, Prov Key Lab Agrobiol, Jiangsu Key Lab Bioresources Saline Soils 2.Jiangsu Prov & Chinese Acad Sci, Inst Bot 3.Slovak Univ Agr, Dept Plant Physiol 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat |
| 推荐引用方式 GB/T 7714 | Zhang, DY,Tong, JF,He, XL,et al. A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress[J]. FRONTIERS IN PLANT SCIENCE,2016,6. |
| APA | Zhang, DY.,Tong, JF.,He, XL.,Xu, ZL.,Xu, L.,...&shaohongbochu@126.com.(2016).A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress.FRONTIERS IN PLANT SCIENCE,6. |
| MLA | Zhang, DY,et al."A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress".FRONTIERS IN PLANT SCIENCE 6(2016). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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