The changes of organelle ultrastructure and Ca2+ homeostasis in maize mesophyll cells during the process of drought-induced leaf senescence

	The changes of organelle ultrastructure and Ca2+ homeostasis in maize mesophyll cells during the process of drought-induced leaf senescence
	Ma, Yuan-Yuan 1,2; Guo, Xiu-Lin 1; Liu, Bin-Hui 5; Liu, Zi-Hui 1; Shao, Hong-Bo 3,4
发表期刊	ELECTRONIC JOURNAL OF BIOTECHNOLOGY
	2011-05-15
卷号	14 期号:3
关键词	Ca2++ Homeostasis Drought Leaf Senescence Maize Organelle Ultrastructure Signal Transduction
英文摘要	The changes of cell ultra structure as well as Ca2+ homeostasis involved in the drought-induced maize leaf senescence was investigated. Meanwhile, many indicatives of leaf senescence including thiobarbituric acid reactive substance (MDA), electrolyte leakage (EL), and chlorophyll along with soluble proteins were also detected during the process. The Polyethylene glycol6000(PEG6000)-incubated detached leaves showed a slight increase in the MDA content and electrolyte leakage during the first 30 min of our detection, which was corresponded to an unobvious alteration of the cell ultrastructure. Other typical senescence parameters measured in whole leaf exhibited a moderate elevation as well. Thereafter, however, the EL and MDA rose to a large extent, which was correlated with a dramatic damage to the cell ultrastructure with concomitant sharp decrease in the chlorophyll and soluble proteins content. The deposits of calcium antimonite, being an indicator for Ca2+ localization, were observed in the vacuoles as well as intercellular spaces in the leaves grown under normal condition. Nevertheless, after PEG treatment, it was revealed a distinct increment of Ca2+ in the cytoplasm as well as chloroplasts and nuclei. Moreover, with long-lasting treatment of PEG to the detached leaves, the concentration of Ca2+ as described above showed a continuous increment which was consist with the remarked alteration of physiological parameters and severe damage to the ultrastructure of cells, all of which indicated the leaf senescence. Such drought-induced leaf senescence might result from a loss of the cell's capability to extrude Ca2+. All above findings give us a good insight into the important role of Ca2+ homeostasis in the process of leaf senescence accelerated by the drought stress.
文章类型	Article
收录类别	SCI
语种	英语
关键词[WOS]	STRESS SIGNALING PATHWAYS ; GENE-EXPRESSION ; LOW-TEMPERATURE ; LIPID-PEROXIDATION ; ABIOTIC STRESS ; PRIMARY LEAVES ; PLANT-CELLS ; CROSS-TALK ; CALCIUM ; ARABIDOPSIS
研究领域[WOS]	Biotechnology & Applied Microbiology
WOS记录号	WOS:000293632300008
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/9253
专题	中国科学院海岸带环境过程与生态修复重点实验室
作者单位	1.Hebei Acad Agr & Forestry Sci, Inst Genet & Physiol, Plant Genet Engn Ctr Hebei Prov, Shijiazhuang, Peoples R China 2.Shijiazhuang Univ, Sch Chem Engn, Shijiazhuang, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Shandong Prov Key Lab Coastal Environm Proc, Yantai, Peoples R China 4.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China 5.Hebei Acad Agr & Forestry Sci, Inst Dryland Farming, Hengshui, Peoples R China
推荐引用方式 GB/T 7714	Ma, Yuan-Yuan,Guo, Xiu-Lin,Liu, Bin-Hui,et al. The changes of organelle ultrastructure and Ca2+ homeostasis in maize mesophyll cells during the process of drought-induced leaf senescence[J]. ELECTRONIC JOURNAL OF BIOTECHNOLOGY,2011,14(3).
APA	Ma, Yuan-Yuan,Guo, Xiu-Lin,Liu, Bin-Hui,Liu, Zi-Hui,&Shao, Hong-Bo.(2011).The changes of organelle ultrastructure and Ca2+ homeostasis in maize mesophyll cells during the process of drought-induced leaf senescence.ELECTRONIC JOURNAL OF BIOTECHNOLOGY,14(3).
MLA	Ma, Yuan-Yuan,et al."The changes of organelle ultrastructure and Ca2+ homeostasis in maize mesophyll cells during the process of drought-induced leaf senescence".ELECTRONIC JOURNAL OF BIOTECHNOLOGY 14.3(2011).