| A novel benzoate-degrading Rhodococcus strain contains three catA genes with one being transcriptionally active during the growth on benzoate | |
Zhao, Kexin ; Guo, Xiaohong; Gong, Jun
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| 发表期刊 | JOURNAL OF ENVIRONMENTAL BIOLOGY
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| 2013-04-01 | |
| 卷号 | 34期号:2页码:401-407 |
| 关键词 | Par Degradation Rhodococcus Cat a Gene Catechol 1 Cloning 2-dioxygenase Transmition |
| 英文摘要 | The gene encoding catechol 1, 2-dioxygenase (catA) is one of the common biomarkers used for evaluation of microbial degradation of aromatic compounds and bioremediation potentials, whereas the expression of the gene has been little studied. The diversity of the catA genes and their expression pattern in a novel Rhodococcus sp. strain LIN isolated from coastal sediments of the Bohai Sea, China, were investigated. We found that this strain exhibited catechol 1, 2-dioxygenase activity when cultivated with benzoate as the sole carbon source. The catA genes were amplified with degenerate primers and sequenced. Sequence analyses show that the strain has three catA genes which are highly similar to each other (> 97%). However, only one gene was transcribed according to reverse transcription-polymerase chain reaction (RT-PCR) analysis. This indicates that RNA-based approaches could better reflect the diversity and scale of the environmental microbial function than DNA-based ones, when sequencing and quantifying (e.g. quantitative PCR) of the catA gene are employed to assess the environmental pollutions and bioremediations. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MARINE ROSEOBACTER LINEAGE ; BETA-KETOADIPATE PATHWAY ; CATECHOL 1,2-DIOXYGENASE ; PHENOL ; ANILINE ; BIODEGRADATION ; DEGRADATION ; BACTERIUM ; IDENTIFICATION ; PURIFICATION |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| WOS记录号 | WOS:000323852700017 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/9052 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Lab Environm Microbiol,Network Lab Coastal Microb, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Kexin,Guo, Xiaohong,Gong, Jun. A novel benzoate-degrading Rhodococcus strain contains three catA genes with one being transcriptionally active during the growth on benzoate[J]. JOURNAL OF ENVIRONMENTAL BIOLOGY,2013,34(2):401-407. |
| APA | Zhao, Kexin,Guo, Xiaohong,&Gong, Jun.(2013).A novel benzoate-degrading Rhodococcus strain contains three catA genes with one being transcriptionally active during the growth on benzoate.JOURNAL OF ENVIRONMENTAL BIOLOGY,34(2),401-407. |
| MLA | Zhao, Kexin,et al."A novel benzoate-degrading Rhodococcus strain contains three catA genes with one being transcriptionally active during the growth on benzoate".JOURNAL OF ENVIRONMENTAL BIOLOGY 34.2(2013):401-407. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 2013-J_Env_Biol.pdf（2339KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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