Optimized Astaxanthin Production in Chlorella zofingiensis under Dark Condition by Response Surface Methodology

doi:10.1007/s10068-013-0221-7

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	Optimized Astaxanthin Production in Chlorella zofingiensis under Dark Condition by Response Surface Methodology
	Chen, Tao 1; Wang, Yan2 ; Yan Wang,Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai, Shandong 264003, China Tel: +86535 2109151 ; Fax: +86535 2109151 E-mail: ywang@yic.ac.cn
发表期刊	FOOD SCIENCE AND BIOTECHNOLOGY
ISSN	1226-7708
	2013-10-01
卷号	22 期号:5 页码:1343-1350
关键词	Astaxanthin Chlorella Zofingiensis Optimization Response Surface Methodology Two-stage Culture
DOI	10.1007/s10068-013-0221-7
产权排序	[TaoChen]College of Food Science and Technology, Huazhong Agricultural University; [Yan Wang]Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	In this study, the major factors affecting both cell growth and astaxanthin production of Chlorella zofingiensis under dark were identified and optimized through statistical methods. A Plackett-Burman design was initially used to evaluate seven culture factors, of which glucose, NaNO3, and MgSO4 center dot 7H(2)O were found to be the most important in affecting C. zofingiensis. The central composite design and response surface methodology (RSM) were further used to optimize these factors. The optimized glucose, NaNO3, and MgSO4 center dot 7H(2)O concentrations for cell growth were 46.7, 1.13, and 0.125 g/L MgSO4 center dot 7H(2)O respectively, and for astaxanthin production were 35.2, 0.281, and 0.023 g/L, respectively. After the optimization, a two-stage culture strategy was employed to further maximize astaxanthin production, and the results showed that the astaxanthin yield of C. zofingiensis could reach 15.1 mg/L, which was 74% higher than that achieved in batch culture using the basal medium (i.e., 8.7 mg/L).
文章类型	Article
收录类别	SCI
语种	英语
关键词[WOS]	ALGA HAEMATOCOCCUS-PLUVIALIS ; GREEN-ALGA ; CAROTENOID PRODUCTION ; HUMAN HEALTH ; ACCUMULATION ; MICROALGAE ; CULTURE ; STRESS ; GROWTH ; NUTRITION
研究领域[WOS]	Food Science & Technology
WOS记录号	WOS:000326044400022
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/9034
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Yan Wang,Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai, Shandong 264003, China Tel: +86535 2109151; Fax: +86535 2109151 E-mail: ywang@yic.ac.cn
作者单位	1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Chen, Tao,Wang, Yan,Yan Wang,Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai, Shandong 264003, China Tel: +86535 2109151,et al. Optimized Astaxanthin Production in Chlorella zofingiensis under Dark Condition by Response Surface Methodology[J]. FOOD SCIENCE AND BIOTECHNOLOGY,2013,22(5):1343-1350.
APA	Chen, Tao,Wang, Yan,Yan Wang,Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai, Shandong 264003, China Tel: +86535 2109151,&Fax: +86535 2109151 E-mail: ywang@yic.ac.cn.(2013).Optimized Astaxanthin Production in Chlorella zofingiensis under Dark Condition by Response Surface Methodology.FOOD SCIENCE AND BIOTECHNOLOGY,22(5),1343-1350.
MLA	Chen, Tao,et al."Optimized Astaxanthin Production in Chlorella zofingiensis under Dark Condition by Response Surface Methodology".FOOD SCIENCE AND BIOTECHNOLOGY 22.5(2013):1343-1350.

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