Characterization of microbial community structure associated with pollution in Xiaoqing River Sediment

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	Characterization of microbial community structure associated with pollution in Xiaoqing River Sediment
	Li, Jialin1 ; Zou, Tao2 ; Yu, Shuxian1,3 ; Qin, Song1
会议主办者	Atlantic Canada Opportunities Agency; et al.; Faculty of Engineering and Applied Science; Newfoundland Labrador Environment and Conservation; RDC; Suncor Energy
	2014
会议名称	International Conference on Marine and Freshwater Environments, iMFE 2014
卷号	INTELLIGENT ROBOTICS AND APPLICATIONS, ICIRA 2014, PT I
会议日期	August 6, 2014 - August 8, 2014
会议地点	St. John's, NL, Canada
产权排序	(1) Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China ; (2) Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China ; (3) Graduate University of Chinese Academy of Sciences, Beijing, China
关键词	River Pollution 16s Rrna Clone Libraries Anthropogenic Activity Dgge Diversity Environmental Gradient Microbial Community Structures Microbial Composition Xiaoqing Rivers
摘要	To understand the impacts of anthropogenic activities on structure and composition of microbial communities and evaluate how microbial communities respond environmental gradients at river sediments, the composition of microbial communities in sediments from Xiaoqing River (in spring, summer and autumn) were assessed using DGGE and real-time PCR approaches. Meanwhile, 16S rRNA clone library construction of three sites was constructed to represent the composition and structure of microbial communities in the three distinct site-groups. The gene copy number was ranged from 107-108 cells/g that was most influenced by sample sediment density and medium grain size. Through analysis of DGGE gel profile, there were no distinct variation on richness, evenness and Shannon-Weaver index with all samples, which ranged 3.69-5.21, 1.73-2.30, and 0.56-0.76 respectively. The clustering result on the DGGE patterns showed that the microbial diversity of all samples were more similar than 40%, while the distinction was formed with three groups at a level of 46% similarity. Redundancy analysis revealed that the distribution of microbial composition seemed to be determined by the variables of nitrite, medium grain size and total carbon content. The clone library of three sites revealed that the Proteobacteria was the dominant phylum, which consistent with DGGE bands sequencing result. In addition, members of Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Planctomycetes and Verrucomicrobia were recorded in all three libraries. The distribution of functional populations as denitrifier and anammox bacteria will be the focus of future research.
作者部门	海岸带生物学与生物资源利用所重点实验室
收录类别	EI
语种	英语
文献类型	会议论文
条目标识符	http://ir.yic.ac.cn/handle/133337/8836
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Qin, Song
作者单位	1.Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences 2.Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences 3.Graduate University of Chinese Academy of Sciences
第一作者单位	中国科学院烟台海岸带研究所
通讯作者单位	中国科学院烟台海岸带研究所
推荐引用方式 GB/T 7714	Li, Jialin,Zou, Tao,Yu, Shuxian,et al. Characterization of microbial community structure associated with pollution in Xiaoqing River Sediment[C]:International Society for Environmental Information Sciences, 4246 Albert Street, Suite 413, Regina, Saskatchewan, S4S 3R9, Canada,2014.