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| Upconversion Fluorescence-SERS Dual-Mode Tags for Cellular and in Vivo Imaging | |
Niu, Xiaojuan1; Chen, Haiyan2; Wang, Yunqing3 ; Wang, Wenhai3; Sun, Xiuyan1; Chen, Lingxin3; Wang, YQ (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. yqwang@yic.ac.cn; lxchen@yic.ac.cn
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| 发表期刊 | ACS APPLIED MATERIALS & INTERFACES
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| ISSN | 1944-8244 |
| 2014-04-09 | |
| 卷号 | 6期号:7页码:5152-5160 |
| 关键词 | Upconversion Nanoparticles Silver Nanoparticles Fluorescence Surface-enhanced Raman Scattering (Sers) Dual-mode Imaging |
| DOI | 10.1021/am500411m |
| 产权排序 | [Niu, Xiaojuan; Sun, Xiuyan] Yantai Univ, Sch Pharm, Yantai 264005, Peoples R China; [Chen, Haiyan] China Pharmaceut Univ, Sch Life Sci & Technol, State Key Lab Nat Med, Nanjing 210009, Peoples R China; [Wang, Yunqing; Wang, Wenhai; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Fluorescent-surface enhanced Raman scattering (F-SERS) dual mode tags showed great potential for bioimaging due to the combined advantages of intuitive, fast imaging of fluorescence and multiplex capability of SERS technique. In previously reported F-SERS tags, organic fluorescent dyes or quantum dots were generally selected to generate fluorescence signal. Herein, we reported the first proof-of-concept upconversion fluorescence (UCF)-SERS dual mode tags based on near infrared (NIR) laser (980 nm) excited upconversion nanoparticles (UCNPs) for live-cell and in vivo imaging. Three components involved in this tag: NaYF4:Yb,Er UCNPs@SiO2 serving as the fluorescent core of the tag; silver nanoparticles in situ grown on the surface of UCNPs@SiO2 for generating characteristic Raman signal; and denatured BSA coating rendering the tag's stability and biocompatibility. The UCF-SERS tags integrated the NIR imaging capability of both fluorescent UCNPs and plasmonic SERS nanoprobe, which facilitated dual mode bioimaging investigation, especially for living animals. Ex vivo experiments revealed that with 980 nm and 785 nm NIR laser irradiations, the UCF and SERS signals of the tags could be detected from 3 and 7 mm deep pork tissues, respectively. Furthermore, the in vivo imaging capabilities of UCF-SERS tags were successfully demonstrated on living mice. The developed dual modality tags held great potential for medical diagnostics and therapy.; Fluorescent-surface enhanced Raman scattering (F-SERS) dual mode tags showed great potential for bioimaging due to the combined advantages of intuitive, fast imaging of fluorescence and multiplex capability of SERS technique. In previously reported F-SERS tags, organic fluorescent dyes or quantum dots were generally selected to generate fluorescence signal. Herein, we reported the first proof-of-concept upconversion fluorescence (UCF)-SERS dual mode tags based on near infrared (NIR) laser (980 nm) excited upconversion nanoparticles (UCNPs) for live-cell and in vivo imaging. Three components involved in this tag: NaYF4:Yb,Er UCNPs@SiO2 serving as the fluorescent core of the tag; silver nanoparticles in situ grown on the surface of UCNPs@SiO2 for generating characteristic Raman signal; and denatured BSA coating rendering the tag's stability and biocompatibility. The UCF-SERS tags integrated the NIR imaging capability of both fluorescent UCNPs and plasmonic SERS nanoprobe, which facilitated dual mode bioimaging investigation, especially for living animals. Ex vivo experiments revealed that with 980 nm and 785 nm NIR laser irradiations, the UCF and SERS signals of the tags could be detected from 3 and 7 mm deep pork tissues, respectively. Furthermore, the in vivo imaging capabilities of UCF-SERS tags were successfully demonstrated on living mice. The developed dual modality tags held great potential for medical diagnostics and therapy. |
| 文章类型 | Article |
| 资助机构 | Science & Technology - Other Topics ; Materials Science |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ENHANCED RAMAN-SCATTERING ; FUNCTIONALIZED SILVER NANOPARTICLES ; UPCONVERTING NANOPARTICLES ; PHOTODYNAMIC THERAPY ; SPECTROSCOPIC DOTS ; DRUG-DELIVERY ; NANOPROBES ; SILICA ; FABRICATION ; NANOSHELLS |
| 研究领域[WOS] | Science & Technology - Other Topics ; Materials Science |
| WOS记录号 | WOS:000334572800069 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8756 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Wang, YQ (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. yqwang@yic.ac.cn; lxchen@yic.ac.cn |
| 作者单位 | 1.Yantai Univ, Sch Pharm, Yantai 264005, Peoples R China 2.China Pharmaceut Univ, Sch Life Sci & Technol, State Key Lab Nat Med, Nanjing 210009, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Niu, Xiaojuan,Chen, Haiyan,Wang, Yunqing,et al. Upconversion Fluorescence-SERS Dual-Mode Tags for Cellular and in Vivo Imaging[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(7):5152-5160. |
| APA | Niu, Xiaojuan.,Chen, Haiyan.,Wang, Yunqing.,Wang, Wenhai.,Sun, Xiuyan.,...&lxchen@yic.ac.cn.(2014).Upconversion Fluorescence-SERS Dual-Mode Tags for Cellular and in Vivo Imaging.ACS APPLIED MATERIALS & INTERFACES,6(7),5152-5160. |
| MLA | Niu, Xiaojuan,et al."Upconversion Fluorescence-SERS Dual-Mode Tags for Cellular and in Vivo Imaging".ACS APPLIED MATERIALS & INTERFACES 6.7(2014):5152-5160. |
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