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| A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing | |
Wang, Xuewei1,2; Yang, Yangang3; Li, Long1,2; Sun, Mingshuang3; Yin, Haogen3; Qin, Wei1 ; Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
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| 发表期刊 | ANALYTICAL CHEMISTRY
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| ISSN | 0003-2700 |
| 2014-05-06 | |
| 卷号 | 86期号:9页码:4416-4422 |
| 关键词 | Graphene Oxide Colorimetric Determination Injection Determination Catalytic-activity Hydrogen-peroxide Dna Immunoassay Benzidine Blood Nanoparticles |
| DOI | 10.1021/ac500281r |
| 产权排序 | [Wang, Xuewei; Li, Long; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China; [Wang, Xuewei; Li, Long] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yang, Yangang; Sun, Mingshuang; Yin, Haogen] Yantai Univ, Coll Chem & Chem Engn, Yantai 264003, Shandong, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | The oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) has great utility in bioanalysis such as peroxidase/peroxidase mimetic-based biosensing. In this paper, the behaviors of TMB oxidation intermediates/products in liquid/liquid biphasic systems have been investigated for the first time. The free radical, charge transfer complex, and diimine species generated by TMB oxidation are all positively charged under acidic and near-neutral conditions. Electron paramagnetic resonance and visible absorbance spectroscopy data demonstrate that these cationic species can be effectively transferred from an aqueous phase into a water-immiscible liquid phase functionalized by an appropriate cation exchanger. Accordingly, sensitive potential responses of TMB oxidation have been obtained on a cation exchanger-doped polymeric liquid membrane electrode under mildly acidic and near-neutral conditions. By using the membrane electrode responsive to TMB oxidations, two sensitive potentiometric biosensing schemes including the peroxidase-labeled sandwich immunoassay and G-quadruplex DNAzyme-based DNA hybridization assay have been developed. The obtained detection limits for the target antigen and DNA are 0.02 ng/mL and 0.1 nM, respectively. Coupled with other advantages such as low cost, high reliability, and ease of miniaturization and integration, the proposed polymeric liquid membrane electrode holds great promise as a facile and efficient transducer for TMB oxidation and related biosensing applications.; The oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) has great utility in bioanalysis such as peroxidase/peroxidase mimetic-based biosensing. In this paper, the behaviors of TMB oxidation intermediates/products in liquid/liquid biphasic systems have been investigated for the first time. The free radical, charge transfer complex, and diimine species generated by TMB oxidation are all positively charged under acidic and near-neutral conditions. Electron paramagnetic resonance and visible absorbance spectroscopy data demonstrate that these cationic species can be effectively transferred from an aqueous phase into a water-immiscible liquid phase functionalized by an appropriate cation exchanger. Accordingly, sensitive potential responses of TMB oxidation have been obtained on a cation exchanger-doped polymeric liquid membrane electrode under mildly acidic and near-neutral conditions. By using the membrane electrode responsive to TMB oxidations, two sensitive potentiometric biosensing schemes including the peroxidase-labeled sandwich immunoassay and G-quadruplex DNAzyme-based DNA hybridization assay have been developed. The obtained detection limits for the target antigen and DNA are 0.02 ng/mL and 0.1 nM, respectively. Coupled with other advantages such as low cost, high reliability, and ease of miniaturization and integration, the proposed polymeric liquid membrane electrode holds great promise as a facile and efficient transducer for TMB oxidation and related biosensing applications. |
| 文章类型 | Article |
| 资助机构 | Chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | GRAPHENE OXIDE ; COLORIMETRIC DETERMINATION ; INJECTION DETERMINATION ; CATALYTIC-ACTIVITY ; HYDROGEN-PEROXIDE ; DNA ; IMMUNOASSAY ; BENZIDINE ; BLOOD ; NANOPARTICLES |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000335719900050 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8747 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yantai Univ, Coll Chem & Chem Engn, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xuewei,Yang, Yangang,Li, Long,et al. A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing[J]. ANALYTICAL CHEMISTRY,2014,86(9):4416-4422. |
| APA | Wang, Xuewei.,Yang, Yangang.,Li, Long.,Sun, Mingshuang.,Yin, Haogen.,...&Qin, W .(2014).A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing.ANALYTICAL CHEMISTRY,86(9),4416-4422. |
| MLA | Wang, Xuewei,et al."A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing".ANALYTICAL CHEMISTRY 86.9(2014):4416-4422. |
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