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A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing
Wang, Xuewei1,2; Yang, Yangang3; Li, Long1,2; Sun, Mingshuang3; Yin, Haogen3; Qin, Wei1; Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
2014-05-06
Volume86Issue:9Pages:4416-4422
KeywordGraphene Oxide Colorimetric Determination Injection Determination Catalytic-activity Hydrogen-peroxide Dna Immunoassay Benzidine Blood Nanoparticles
DOI10.1021/ac500281r
Contribution Rank[Wang, Xuewei; Li, Long; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China; [Wang, Xuewei; Li, Long] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yang, Yangang; Sun, Mingshuang; Yin, Haogen] Yantai Univ, Coll Chem & Chem Engn, Yantai 264003, Shandong, Peoples R China
Department中科院海岸带环境过程与生态修复重点实验室
AbstractThe oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) has great utility in bioanalysis such as peroxidase/peroxidase mimetic-based biosensing. In this paper, the behaviors of TMB oxidation intermediates/products in liquid/liquid biphasic systems have been investigated for the first time. The free radical, charge transfer complex, and diimine species generated by TMB oxidation are all positively charged under acidic and near-neutral conditions. Electron paramagnetic resonance and visible absorbance spectroscopy data demonstrate that these cationic species can be effectively transferred from an aqueous phase into a water-immiscible liquid phase functionalized by an appropriate cation exchanger. Accordingly, sensitive potential responses of TMB oxidation have been obtained on a cation exchanger-doped polymeric liquid membrane electrode under mildly acidic and near-neutral conditions. By using the membrane electrode responsive to TMB oxidations, two sensitive potentiometric biosensing schemes including the peroxidase-labeled sandwich immunoassay and G-quadruplex DNAzyme-based DNA hybridization assay have been developed. The obtained detection limits for the target antigen and DNA are 0.02 ng/mL and 0.1 nM, respectively. Coupled with other advantages such as low cost, high reliability, and ease of miniaturization and integration, the proposed polymeric liquid membrane electrode holds great promise as a facile and efficient transducer for TMB oxidation and related biosensing applications.; The oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) has great utility in bioanalysis such as peroxidase/peroxidase mimetic-based biosensing. In this paper, the behaviors of TMB oxidation intermediates/products in liquid/liquid biphasic systems have been investigated for the first time. The free radical, charge transfer complex, and diimine species generated by TMB oxidation are all positively charged under acidic and near-neutral conditions. Electron paramagnetic resonance and visible absorbance spectroscopy data demonstrate that these cationic species can be effectively transferred from an aqueous phase into a water-immiscible liquid phase functionalized by an appropriate cation exchanger. Accordingly, sensitive potential responses of TMB oxidation have been obtained on a cation exchanger-doped polymeric liquid membrane electrode under mildly acidic and near-neutral conditions. By using the membrane electrode responsive to TMB oxidations, two sensitive potentiometric biosensing schemes including the peroxidase-labeled sandwich immunoassay and G-quadruplex DNAzyme-based DNA hybridization assay have been developed. The obtained detection limits for the target antigen and DNA are 0.02 ng/mL and 0.1 nM, respectively. Coupled with other advantages such as low cost, high reliability, and ease of miniaturization and integration, the proposed polymeric liquid membrane electrode holds great promise as a facile and efficient transducer for TMB oxidation and related biosensing applications.
Funding OrganizationChemistry
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordGRAPHENE OXIDE ; COLORIMETRIC DETERMINATION ; INJECTION DETERMINATION ; CATALYTIC-ACTIVITY ; HYDROGEN-PEROXIDE ; DNA ; IMMUNOASSAY ; BENZIDINE ; BLOOD ; NANOPARTICLES
WOS Research AreaChemistry
WOS IDWOS:000335719900050
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8747
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorQin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Yantai Univ, Coll Chem & Chem Engn, Yantai 264003, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xuewei,Yang, Yangang,Li, Long,et al. A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing[J]. ANALYTICAL CHEMISTRY,2014,86(9):4416-4422.
APA Wang, Xuewei.,Yang, Yangang.,Li, Long.,Sun, Mingshuang.,Yin, Haogen.,...&Qin, W .(2014).A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing.ANALYTICAL CHEMISTRY,86(9),4416-4422.
MLA Wang, Xuewei,et al."A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing".ANALYTICAL CHEMISTRY 86.9(2014):4416-4422.
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