Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

doi:10.1016/j.snb.2014.04.012

	Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design
	Ding, Jiawang1 ; Zhang, Hongxia 1; Jia, Falong 2; Qin, Wei1 ; Du, Dan 2
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
ISSN	0925-4005
	2014-08-01
卷号	199 页码:284-290
关键词	Nanoporous Gold Carbon Nanotubes Amperometry Acetylcholinesterase Pesticides
DOI	10.1016/j.snb.2014.04.012
英文摘要	An electrochemical sensing platform based on assembly of carbon nanotubes on a nanoporous gold electrode is described for highly sensitive detection of organophosphate pesticides. The nanoporous gold film (NPG) electrode is fabricated by an alloying/dealloying process, which possess high electroactive surface area and is an excellent substrate for sensor design. The NPG functionalized with cysteamine allows the immobilization of carbon nanotubes on the electrode with the self-assembly technique. The carboxylated carbon nanotubes are further linkered with acetylcholinesterase (AChE) for amperometric sensing of pesticides. The immobilized AChE, as a model, shows excellent activity to its substrate and allows a quantitative measurement of organophosphate pesticides. Under the optimal experimental conditions, the inhibition of malathion is proportional to its concentration in the range of 0.001-0.5 mu g mL(-1) with a detection limit of 0.5 ng mL(-1). The proposed method shows good reproducibility and high stability, which provides a new avenue for electrochemical biosensor design. (C) 2014 Elsevier B.V. All rights reserved.
文章类型	Article
资助机构	National Natural Science Foundation of China(21207156) ; Science and Technology Project of Yantai(2012132) ; Taishan Scholar Program of Shandong Province(TS20081159)
收录类别	SCI
语种	英语
关键词[WOS]	RAT-BRAIN ACETYLCHOLINESTERASE ; LINKED CHITOSAN COMPOSITE ; MODIFIED AU ELECTRODE ; ORGANOPHOSPHATE PESTICIDES ; ELECTROCHEMICAL PROPERTIES ; FILM ELECTRODES ; SURFACE-AREA ; POROUS GOLD ; NANOPARTICLES ; IMMOBILIZATION
研究领域[WOS]	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000336878000040
引用统计	被引频次：49[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/8724
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Qin, Wei
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, YICCAS, Yantai Inst Coastal Zone Res YIC,Key Lab Coastal, Yantai 264003, Shandong, Peoples R China 2.Huazhong Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China
推荐引用方式 GB/T 7714	Ding, Jiawang,Zhang, Hongxia,Jia, Falong,et al. Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,199:284-290.
APA	Ding, Jiawang,Zhang, Hongxia,Jia, Falong,Qin, Wei,&Du, Dan.(2014).Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design.SENSORS AND ACTUATORS B-CHEMICAL,199,284-290.
MLA	Ding, Jiawang,et al."Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design".SENSORS AND ACTUATORS B-CHEMICAL 199(2014):284-290.

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