Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal | |
Zhang, Wei Wei1; Andong, Zheng Yang2; Zhang, Min3; Wang, Qiao Ning1; Wei, Ying Qin4; Chen, Ling Xin1 ; Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China. wwzhang@yic.ac.cn; Andongzhengyang123@163.com; zhangmin@qdio.ac.cn; wangqiaoning333@hotmail.com; wyq@qlu.edu.cn; lxchen@yic.ac.cn
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| 发表期刊 | ANNALS OF MICROBIOLOGY
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| ISSN | 1590-4261 |
| 2014-09-01 | |
| 卷号 | 64期号:3页码:1231-1238 |
| 关键词 | Ammonia Proteus Mirabilis nh4+-n Removal Ammonia Monooxygenase Mrna Level |
| DOI | 10.1007/s13213-013-0764-0 |
| 产权排序 | [Zhang, Wei Wei; Wang, Qiao Ning; Chen, Ling Xin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Andong, Zheng Yang] China Agr Univ Yantai, Dept Aquaculture, Yantai 264670, Shandong, Peoples R China; [Zhang, Min] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China; [Wei, Ying Qin] Qilu Univ Technol, Sch Chem & Pharmaceut Engn, Jinan 250353, Shandong, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Ammonia (referring to both NH3 and NH4 (+)), is one of the most toxic nitrogen forms, and is toxic to many aquatic organisms. The present study identified a heterotrophic nitrifier Proteus mirabilis strain V7, isolated from the coastal seawater. Almost 100 % of 82 mg L-1 NH4 (+)-N was removed within 48 h, while 51.2 % of total inorganic nitrogen was removed within 96 h after inoculation of Proteus mirabilis strain V7. Moreover, Proteus mirabilis strain V7 could remove NH4 (+)-N in a seawater matrix, but with much lower efficiency. Three water samples containing NH4 (+)-N at concentrations of 64.40, 18.00 and 9.82 mg L-1 were chosen to further test the NH4 (+)-N removal ability of Proteus mirabilis strain V7. All of the NH4 (+)-N was removed, with only a trace production of NO2 (-) and NO3 (-). A putative subunit of ammonia monooxygenase from Proteus mirabilis strain V7, amoA (Pm), was cloned and its mRNA level in the presence of NH4 (+) was characterized by real time reverse transcriptase PCR (RT-PCR). A 1.8-fold increase in the mRNA level of amoA (Pm) was observed, which indicated that AmoA may be involved in NH4 (+) oxidization. Our results indicated that Proteus mirabilis strain V7 could potentially be used as new resource for the treatment of NH4 (+)-N contaminated environments.; Ammonia (referring to both NH3 and NH4 (+)), is one of the most toxic nitrogen forms, and is toxic to many aquatic organisms. The present study identified a heterotrophic nitrifier Proteus mirabilis strain V7, isolated from the coastal seawater. Almost 100 % of 82 mg L-1 NH4 (+)-N was removed within 48 h, while 51.2 % of total inorganic nitrogen was removed within 96 h after inoculation of Proteus mirabilis strain V7. Moreover, Proteus mirabilis strain V7 could remove NH4 (+)-N in a seawater matrix, but with much lower efficiency. Three water samples containing NH4 (+)-N at concentrations of 64.40, 18.00 and 9.82 mg L-1 were chosen to further test the NH4 (+)-N removal ability of Proteus mirabilis strain V7. All of the NH4 (+)-N was removed, with only a trace production of NO2 (-) and NO3 (-). A putative subunit of ammonia monooxygenase from Proteus mirabilis strain V7, amoA (Pm), was cloned and its mRNA level in the presence of NH4 (+) was characterized by real time reverse transcriptase PCR (RT-PCR). A 1.8-fold increase in the mRNA level of amoA (Pm) was observed, which indicated that AmoA may be involved in NH4 (+) oxidization. Our results indicated that Proteus mirabilis strain V7 could potentially be used as new resource for the treatment of NH4 (+)-N contaminated environments. |
| 文章类型 | Article |
| 资助机构 | Biotechnology & Applied Microbiology ; Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | NITRIFYING-DENITRIFYING BACTERIUM ; AEROBIC DENITRIFICATION ; THIOSPHAERA-PANTOTROPHA ; AMMONIA MONOOXYGENASE ; NITROSOMONAS-EUROPAEA ; HYDROXYLAMINE OXIDOREDUCTASE ; SIMULTANEOUS NITRIFICATION ; ALCALIGENES-FAECALIS ; NITROGEN REMOVAL ; WASTE-WATER |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| WOS记录号 | WOS:000340087200037 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8713 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China. wwzhang@yic.ac.cn; Andongzhengyang123@163.com; zhangmin@qdio.ac.cn; wangqiaoning333@hotmail.com; wyq@qlu.edu.cn; lxchen@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 2.China Agr Univ Yantai, Dept Aquaculture, Yantai 264670, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 4.Qilu Univ Technol, Sch Chem & Pharmaceut Engn, Jinan 250353, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wei Wei,Andong, Zheng Yang,Zhang, Min,et al. Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal[J]. ANNALS OF MICROBIOLOGY,2014,64(3):1231-1238. |
| APA | Zhang, Wei Wei.,Andong, Zheng Yang.,Zhang, Min.,Wang, Qiao Ning.,Wei, Ying Qin.,...&lxchen@yic.ac.cn.(2014).Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal.ANNALS OF MICROBIOLOGY,64(3),1231-1238. |
| MLA | Zhang, Wei Wei,et al."Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal".ANNALS OF MICROBIOLOGY 64.3(2014):1231-1238. |
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