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| Cloning and expression of a transcription factor activator protein-1 (AP-1) member identified from manila clam Venerupis philippinarum | |
Wu, Luning1,2; Zhang, Lei2; Zhao, Jianmin2 ; Ning, Xuanxuan3; Mu, Changkao1; Wang, Chunlin1; Zhao, JM (reprint author), Chinese Acad Sci, Yantai Coastal Zone Res Inst, 17 Chunhui Rd, Laishan Dist 264003, Yantai, Peoples R China. jmzhao@yic.ac.cn; wangchunlin@nbu.edu.cn
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| 发表期刊 | GENE
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| ISSN | 0378-1119 |
| 2015-02-15 | |
| 卷号 | 557期号:1页码:106-111 |
| 关键词 | Transcription Factor Activator Protein-1 Venerupis Philippinarum Immune Response Bacterial Challenge |
| DOI | 10.1016/j.gene.2014.12.027 |
| 产权排序 | [Wu, Luning; Mu, Changkao; Wang, Chunlin] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China; [Wu, Luning; Zhang, Lei; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China; [Ning, Xuanxuan] State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | The transcription factor activator protein-1 (AP-1) proteins are implicated to play a major role in the regulation of numerous genes involved in the function and development of the immune system, cell differentiation, proliferation, apoptosis, etc. It can bind to promoter of its target genes in a sequence-specific manner to transactivate or repress them. In this study, the full-length cDNA of an AP-1 was identified from Venerupis philippinarum (denoted as VpAP-1) by EST analysis and RACE approaches. Phylogenetic analysis indicated that VpAP-1 had higher evolutional conservation to invertebrate than vertebrate counterparts and should be a new member of the AP-1 protein family. Spatial expression analysis found that VpAP-1 transcript was most abundantly expressed in the hemocytes and hepatopancreas, weakly expressed in the tissues of the gills, mantle and muscle. After Vibrio anguillarum challenge, the expression of VpAP-1 transcript in overall hemocyte population was up-regulated in the first 6 h, and then decreased to 1.5-fold of the control group at 12 h. As time progressed, a second peak of VpAP-1 expression was detected at 24 h post-infection, which was 5-fold compared with that of the control group (P < 0.01). After that, the expression level was sharply decreased and dropped to 0.5-fold of the control at 96 h. The above results indicated that VpAP-1 was perhaps involved in the immune responses against microbe infection and might be contributed to the clearance of bacterial pathogens. (C) 2014 Published by Elsevier B.V.; The transcription factor activator protein-1 (AP-1) proteins are implicated to play a major role in the regulation of numerous genes involved in the function and development of the immune system, cell differentiation, proliferation, apoptosis, etc. It can bind to promoter of its target genes in a sequence-specific manner to transactivate or repress them. In this study, the full-length cDNA of an AP-1 was identified from Venerupis philippinarum (denoted as VpAP-1) by EST analysis and RACE approaches. Phylogenetic analysis indicated that VpAP-1 had higher evolutional conservation to invertebrate than vertebrate counterparts and should be a new member of the AP-1 protein family. Spatial expression analysis found that VpAP-1 transcript was most abundantly expressed in the hemocytes and hepatopancreas, weakly expressed in the tissues of the gills, mantle and muscle. After Vibrio anguillarum challenge, the expression of VpAP-1 transcript in overall hemocyte population was up-regulated in the first 6 h, and then decreased to 1.5-fold of the control group at 12 h. As time progressed, a second peak of VpAP-1 expression was detected at 24 h post-infection, which was 5-fold compared with that of the control group (P < 0.01). After that, the expression level was sharply decreased and dropped to 0.5-fold of the control at 96 h. The above results indicated that VpAP-1 was perhaps involved in the immune responses against microbe infection and might be contributed to the clearance of bacterial pathogens. (C) 2014 Published by Elsevier B.V. |
| 文章类型 | Article |
| 资助机构 | Genetics & Heredity |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MARINE-INVERTEBRATES ; CELL-PROLIFERATION ; MOLECULAR-CLONING ; GENE ; FOS ; JUN |
| 研究领域[WOS] | Genetics & Heredity |
| WOS记录号 | WOS:000348885900015 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8653 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Zhao, JM (reprint author), Chinese Acad Sci, Yantai Coastal Zone Res Inst, 17 Chunhui Rd, Laishan Dist 264003, Yantai, Peoples R China. jmzhao@yic.ac.cn; wangchunlin@nbu.edu.cn |
| 作者单位 | 1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China 3.State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Luning,Zhang, Lei,Zhao, Jianmin,et al. Cloning and expression of a transcription factor activator protein-1 (AP-1) member identified from manila clam Venerupis philippinarum[J]. GENE,2015,557(1):106-111. |
| APA | Wu, Luning.,Zhang, Lei.,Zhao, Jianmin.,Ning, Xuanxuan.,Mu, Changkao.,...&wangchunlin@nbu.edu.cn.(2015).Cloning and expression of a transcription factor activator protein-1 (AP-1) member identified from manila clam Venerupis philippinarum.GENE,557(1),106-111. |
| MLA | Wu, Luning,et al."Cloning and expression of a transcription factor activator protein-1 (AP-1) member identified from manila clam Venerupis philippinarum".GENE 557.1(2015):106-111. |
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