Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Quantum Dots Based Mesoporous Structured Imprinting Microspheres for the Sensitive Fluorescent Detection of Phycocyanin | |
Zhang, Zhong1,3; Li, Jinhua1 ; Wang, Xiaoyan1,2; Shen, Dazhong2; Chen, Lingxin1; Chen, LX (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
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| 发表期刊 | ACS APPLIED MATERIALS & INTERFACES
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| ISSN | 1944-8244 |
| 2015-05-06 | |
| 卷号 | 7期号:17页码:9118-9127 |
| 关键词 | Quantum Dots Fluorescent Detection Molecularly Imprinted Polymers Mesoporous Structure Phycocyanin |
| DOI | 10.1021/acsami.5b00908 |
| 产权排序 | [Zhang, Zhong; Li, Jinhua; Wang, Xiaoyan; Chen, Lingxin] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wang, Xiaoyan; Shen, Dazhong] Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nanomat Probes, Minist Educ China, Jinan 250014, Peoples R China; [Zhang, Zhong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Phycocyanin with important physiological/environmental significance has attracted increasing attention; versatile molecularly imprinted polymers (MIPs) have been applied to diverse species, but protein imprinting is still quite difficult. Herein, using phycocyanin as template via a sol-gel process, we developed a novel fluorescent probe for specific recognition and sensitive detection of phycocyanin by quantum dots (QDs) based mesoporous structured imprinting microspheres (SiO2@QDs@ms-MIPs), obeying electron-transfer-induced fluorescence quenching mechanism. When phycocyanin was present, a Meisenheimer complex would be produced between phycocyanin and primary amino groups of QDs surface, and then the photoluminescent energy of QDs would be transferred to the complex, leading to the fluorescence quenching of QDs. As a result, the fluorescent intensity of the SiO2@QDs@ms-MIPs was significantly decreased within 8 min, and accordingly a favorable linearity within 0.02-0.8 mu M and a high detectability of 5.9 nM were presented. Excellent recognition specificity for phycocyanin over its analogues was displayed, with a high imprinting factor of 4.72. Furthermore, the validated probe strategy was successfully applied to seawater and lake water sample analysis, and high recoveries in the range of 94.0105.0% were attained at three spiking levels of phycocyanin, with precisions below 5.3%. The study provided promising perspectives to develop fluorescent probes for convenient, rapid recognition and sensitive detection of trace proteins from complex matrices, and further pushed forward protein imprinting research.; Phycocyanin with important physiological/environmental significance has attracted increasing attention; versatile molecularly imprinted polymers (MIPs) have been applied to diverse species, but protein imprinting is still quite difficult. Herein, using phycocyanin as template via a sol-gel process, we developed a novel fluorescent probe for specific recognition and sensitive detection of phycocyanin by quantum dots (QDs) based mesoporous structured imprinting microspheres (SiO2@QDs@ms-MIPs), obeying electron-transfer-induced fluorescence quenching mechanism. When phycocyanin was present, a Meisenheimer complex would be produced between phycocyanin and primary amino groups of QDs surface, and then the photoluminescent energy of QDs would be transferred to the complex, leading to the fluorescence quenching of QDs. As a result, the fluorescent intensity of the SiO2@QDs@ms-MIPs was significantly decreased within 8 min, and accordingly a favorable linearity within 0.02-0.8 mu M and a high detectability of 5.9 nM were presented. Excellent recognition specificity for phycocyanin over its analogues was displayed, with a high imprinting factor of 4.72. Furthermore, the validated probe strategy was successfully applied to seawater and lake water sample analysis, and high recoveries in the range of 94.0105.0% were attained at three spiking levels of phycocyanin, with precisions below 5.3%. The study provided promising perspectives to develop fluorescent probes for convenient, rapid recognition and sensitive detection of trace proteins from complex matrices, and further pushed forward protein imprinting research. |
| 文章类型 | Article |
| 资助机构 | Science & Technology - Other Topics ; Materials Science |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SILICA ; RECOGNITION ; POLYMERS ; PROBES ; NANOPARTICLES ; IMMUNOASSAY ; THIOLS |
| 研究领域[WOS] | Science & Technology - Other Topics ; Materials Science |
| WOS记录号 | WOS:000354338800022 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8611 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nanomat Probes, Minist Educ China, Jinan 250014, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhong,Li, Jinhua,Wang, Xiaoyan,et al. Quantum Dots Based Mesoporous Structured Imprinting Microspheres for the Sensitive Fluorescent Detection of Phycocyanin[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(17):9118-9127. |
| APA | Zhang, Zhong,Li, Jinhua,Wang, Xiaoyan,Shen, Dazhong,Chen, Lingxin,&Chen, LX .(2015).Quantum Dots Based Mesoporous Structured Imprinting Microspheres for the Sensitive Fluorescent Detection of Phycocyanin.ACS APPLIED MATERIALS & INTERFACES,7(17),9118-9127. |
| MLA | Zhang, Zhong,et al."Quantum Dots Based Mesoporous Structured Imprinting Microspheres for the Sensitive Fluorescent Detection of Phycocyanin".ACS APPLIED MATERIALS & INTERFACES 7.17(2015):9118-9127. |
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