| Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris | |
Liu, Zhengyi1 ; Zhang, Lei2; Pu, Yang2; Liu, Zhaopu1; Li, Zhiling3; Zhao, Yushan3; Qin, Song1; Qin, S (reprint author), Nanjing Agr Univ, Key Lab Marine Biol Jiang Su, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China. sqin@yic.ac.cn
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| 发表期刊 | BIOMED RESEARCH INTERNATIONAL
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| ISSN | 2314-6133 |
| 2014 | |
| 页码 | 487050 |
| 关键词 | Chlamydomonas-reinhardtii Arabidopsis-thaliana Sequence Alignment Stress Tolerance Heat-stress Family Shock Chloroplast Evolutionary Proteins |
| DOI | 10.1155/2014/487050 |
| 产权排序 | [Liu, Zhengyi; Liu, Zhaopu; Qin, Song] Nanjing Agr Univ, Key Lab Marine Biol Jiang Su, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China; [Zhang, Lei; Pu, Yang] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Li, Zhiling; Zhao, Yushan] Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Heat shock protein 90 (HSP90), a highly conserved molecular chaperone, plays essential roles in folding, keeping structural integrity, and regulating the subset of cytosolic proteins. We cloned the cDNA of Chlorella vulgaris HSP90 (named CvHSP90) by combining homology cloning with rapid amplification of cDNA ends (RACE). Sequence analysis indicated that CvHSP90 is a cytosolic member of the HSP90 family. Quantitative RT-PCR was applied to determine the expression level of messenger RNA (mRNA) in CvHSP90 under different stress conditions. C. vulgaris was kept in different temperatures (5-45 degrees C) for 1 h. The mRNA expression level of CvHSP90 increased with temperature from 5 to 10 degrees C, went further from 35 to 40 degrees C, and reached the maximum at 40 degrees C. On the other hand, for C. vulgaris kept at 35 degrees C for different durations, the mRNA expression level of CvHSP90 increased gradually and reached the peak at 7 h and then declined progressively. In addition, the expression level of CvHSP90 at 40 or 45 in salinity (parts per thousand) was almost fourfold of that at 25 in salinity (parts per thousand) for 2 h. Therefore, CvHSP90 may be a potential biomarker to monitor environment changes.; Heat shock protein 90 (HSP90), a highly conserved molecular chaperone, plays essential roles in folding, keeping structural integrity, and regulating the subset of cytosolic proteins. We cloned the cDNA of Chlorella vulgaris HSP90 (named CvHSP90) by combining homology cloning with rapid amplification of cDNA ends (RACE). Sequence analysis indicated that CvHSP90 is a cytosolic member of the HSP90 family. Quantitative RT-PCR was applied to determine the expression level of messenger RNA (mRNA) in CvHSP90 under different stress conditions. C. vulgaris was kept in different temperatures (5-45 degrees C) for 1 h. The mRNA expression level of CvHSP90 increased with temperature from 5 to 10 degrees C, went further from 35 to 40 degrees C, and reached the maximum at 40 degrees C. On the other hand, for C. vulgaris kept at 35 degrees C for different durations, the mRNA expression level of CvHSP90 increased gradually and reached the peak at 7 h and then declined progressively. In addition, the expression level of CvHSP90 at 40 or 45 in salinity (parts per thousand) was almost fourfold of that at 25 in salinity (parts per thousand) for 2 h. Therefore, CvHSP90 may be a potential biomarker to monitor environment changes. |
| 文章类型 | Article |
| 资助机构 | Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CHLAMYDOMONAS-REINHARDTII ; ARABIDOPSIS-THALIANA ; SEQUENCE ALIGNMENT ; STRESS TOLERANCE ; HEAT-STRESS ; FAMILY ; SHOCK ; CHLOROPLAST ; EVOLUTIONARY ; PROTEINS |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
| WOS记录号 | WOS:000333346100001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8583 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Qin, S (reprint author), Nanjing Agr Univ, Key Lab Marine Biol Jiang Su, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China. sqin@yic.ac.cn |
| 作者单位 | 1.Nanjing Agr Univ, Key Lab Marine Biol Jiang Su, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhengyi,Zhang, Lei,Pu, Yang,et al. Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris[J]. BIOMED RESEARCH INTERNATIONAL,2014:487050. |
| APA | Liu, Zhengyi.,Zhang, Lei.,Pu, Yang.,Liu, Zhaopu.,Li, Zhiling.,...&Qin, S .(2014).Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris.BIOMED RESEARCH INTERNATIONAL,487050. |
| MLA | Liu, Zhengyi,et al."Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris".BIOMED RESEARCH INTERNATIONAL (2014):487050. |
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