| Optimizing medium for producing ethanol from industrial crop Jerusalem artichoke by one- step fermentation and recombinant Saccharomyces cerevisiae | |
| Du, Y. C.1,2; Long, X. H.1; Liu, Z. P.1; Shao, H. B.1,2,3; Long, XH (reprint author), Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China. longxiaohua@njau.edu.cn; zhaopuliu@163.com; shaohongbochu@126.com | |
| 发表期刊 | PLANT BIOSYSTEMS
 |
| ISSN | 1126-3504 |
| 2014-01-02 | |
| 卷号 | 148期号:1页码:118-126 |
| 关键词 | Rsm Optimization Jerusalem Artichoke Energy Saccharomyces Cerevisiae One-step Fermentation |
| DOI | 10.1080/11263504.2013.852630 |
| 产权排序 | [Du, Y. C.; Long, X. H.; Liu, Z. P.; Shao, H. B.] Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China; [Du, Y. C.; Shao, H. B.] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; [Shao, H. B.] Qingdao Univ Sci & Technol QUST, Inst Life Sci, Qingdao 266042, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | In order to obtain a high ethanol yield from the Jerusalem artichoke raw extract and reduce the fermentation cost, we have engineered a new recombinant Saccharomyces cerevisiae strain that could produce ex-inulinase. The response surface methodology based on Plackett-Burman and Box-Behnken design was used to optimize the medium for the ethanol production from the Jerusalem artichoke raw extracts by the recombinant strain. In the first optimization step, Plackett-Burman design was employed to select significant factors, including concentrations of yeast extract, inoculum, and MgSO(4)7H(2)O. In the second step, the steepest ascent experiment was carried out to determine the center point with the three significant factors; the selected combinations were further optimized using the Box-Behnken design. The maximum ethanol production rate was predicted at 91.1g/l, which was based on a medium consisting of yeast extract 9.24g/l, inoculum 39.8ml/l, and MgSO(4)7H(2)O 0.45g/l. In the validating experiment, the ethanol fermentation rate reached 102.1g/l, closely matching the predicted rate.; In order to obtain a high ethanol yield from the Jerusalem artichoke raw extract and reduce the fermentation cost, we have engineered a new recombinant Saccharomyces cerevisiae strain that could produce ex-inulinase. The response surface methodology based on Plackett-Burman and Box-Behnken design was used to optimize the medium for the ethanol production from the Jerusalem artichoke raw extracts by the recombinant strain. In the first optimization step, Plackett-Burman design was employed to select significant factors, including concentrations of yeast extract, inoculum, and MgSO(4)7H(2)O. In the second step, the steepest ascent experiment was carried out to determine the center point with the three significant factors; the selected combinations were further optimized using the Box-Behnken design. The maximum ethanol production rate was predicted at 91.1g/l, which was based on a medium consisting of yeast extract 9.24g/l, inoculum 39.8ml/l, and MgSO(4)7H(2)O 0.45g/l. In the validating experiment, the ethanol fermentation rate reached 102.1g/l, closely matching the predicted rate. |
| 文章类型 | Article |
| 资助机构 | Plant Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | RESPONSE-SURFACE METHODOLOGY ; SOLID-STATE MEDIUM ; INULIN HYDROLYSIS ; OPTIMIZATION ; BIOFUELS ; MARKERS ; SUGAR ; L. |
| 研究领域[WOS] | Plant Sciences |
| WOS记录号 | WOS:000333880000017 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8562 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Long, XH (reprint author), Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China. longxiaohua@njau.edu.cn; zhaopuliu@163.com; shaohongbochu@126.com |
| 作者单位 | 1.Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China 3.Qingdao Univ Sci & Technol QUST, Inst Life Sci, Qingdao 266042, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Y. C.,Long, X. H.,Liu, Z. P.,et al. Optimizing medium for producing ethanol from industrial crop Jerusalem artichoke by one- step fermentation and recombinant Saccharomyces cerevisiae[J]. PLANT BIOSYSTEMS,2014,148(1):118-126. |
| APA | Du, Y. C..,Long, X. H..,Liu, Z. P..,Shao, H. B..,Long, XH .,...&shaohongbochu@126.com.(2014).Optimizing medium for producing ethanol from industrial crop Jerusalem artichoke by one- step fermentation and recombinant Saccharomyces cerevisiae.PLANT BIOSYSTEMS,148(1),118-126. |
| MLA | Du, Y. C.,et al."Optimizing medium for producing ethanol from industrial crop Jerusalem artichoke by one- step fermentation and recombinant Saccharomyces cerevisiae".PLANT BIOSYSTEMS 148.1(2014):118-126. |
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