Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community
Yi, Neng1,2; Gao, Yan2; Long, Xiao-hua1; Zhang, Zhi-yong2; Guo, Jun-yao2; Shao, Hong-bo1,3; Zhang, Zhen-hua2; Yan, Shao-hua1,2; Yan, SH (reprint author), Nanjing Agr Univ, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China. shaohongbochu@126.com; shyan@jaas.ac.cn
2014-05-01
发表期刊CLEAN-SOIL AIR WATER
ISSN1863-0650
卷号42期号:5页码:664-673
产权排序[Yi, Neng; Long, Xiao-hua; Shao, Hong-bo; Yan, Shao-hua] Nanjing Agr Univ, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China; [Yi, Neng; Gao, Yan; Zhang, Zhi-yong; Guo, Jun-yao; Zhang, Zhen-hua; Yan, Shao-hua] Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjng, Peoples R China; [Shao, Hong-bo] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai, Peoples R China
摘要The role of floating macrophytes on modulating the microbial nitrogen removal is not well understood. In this study, the cultivation of Eichhornia crassipes in eutrophic water may affect the nitrogen (N) fate by modulating the denitrifying bacteria diversity and abundance. The gaseous N losses via denitrification were estimated by 15N stable isotope tracing and the diversity and abundance of denitrifying genes (nirS, nirK, and nosZ) were investigated by molecular tools. The denaturing gradient gel electrophoresis (DGGE) profiles showed that the diversity of denitrifying genes in the treatments with E. crassipes was significantly higher than that in the treatment without E. crassipes. The real-time PCR (qPCR) results showed the trend of denitrifier abundance in the entire system was in the order of N-ER (nitrate with just root of E. crassipes) and A-ER (ammonia with just root of E. crassipes)>N-R (nitrate with E. crassipes) and A-R (ammonia with E. crassipes)>N-W (nitrate without plant) and A-W (ammonia without plant). The gaseous 15N losses via denitrification were significantly and positively related to the abundance of nirK, nirS, and nosZ genes. The results indicated that cultivation of E. crassipes in eutrophic water could increase the diversity and abundance of denitrifying bacteria, resulting in more N being removed as gases via denitrification.; The role of floating macrophytes on modulating the microbial nitrogen removal is not well understood. In this study, the cultivation of Eichhornia crassipes in eutrophic water may affect the nitrogen (N) fate by modulating the denitrifying bacteria diversity and abundance. The gaseous N losses via denitrification were estimated by 15N stable isotope tracing and the diversity and abundance of denitrifying genes (nirS, nirK, and nosZ) were investigated by molecular tools. The denaturing gradient gel electrophoresis (DGGE) profiles showed that the diversity of denitrifying genes in the treatments with E. crassipes was significantly higher than that in the treatment without E. crassipes. The real-time PCR (qPCR) results showed the trend of denitrifier abundance in the entire system was in the order of N-ER (nitrate with just root of E. crassipes) and A-ER (ammonia with just root of E. crassipes)>N-R (nitrate with E. crassipes) and A-R (ammonia with E. crassipes)>N-W (nitrate without plant) and A-W (ammonia without plant). The gaseous 15N losses via denitrification were significantly and positively related to the abundance of nirK, nirS, and nosZ genes. The results indicated that cultivation of E. crassipes in eutrophic water could increase the diversity and abundance of denitrifying bacteria, resulting in more N being removed as gases via denitrification.
关键词Denitrification Gene Dgge 15n Stable Isotope Tracing Qpcr Water Hyacinth Cleaning
作者部门海岸带生物学与生物资源利用所重点实验室
学科领域Environmental Sciences ; Marine & Freshwater Biology ; Water Resources
DOI10.1002/clen.201300211
项目资助者Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources
收录类别SCI
关键词[WOS]CONSTRUCTED WETLANDS ; OXIDE REDUCTASE ; WATER-QUALITY ; SUBMERGED MACROPHYTES ; DENITRIFICATION GENES ; AGRICULTURAL SOIL ; N2O EMISSIONS ; FRESH-WATER ; NOSZ GENES ; NIRK
文章类型Article
语种英语
WOS研究方向Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources
WOS记录号WOS:000337514900017
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/8551
专题海岸带生物学与生物资源利用所重点实验室
通讯作者Yan, SH (reprint author), Nanjing Agr Univ, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China. shaohongbochu@126.com; shyan@jaas.ac.cn
作者单位1.Nanjing Agr Univ, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjng, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai, Peoples R China
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Yi, Neng,Gao, Yan,Long, Xiao-hua,et al. Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community[J]. CLEAN-SOIL AIR WATER,2014,42(5):664-673.
APA Yi, Neng.,Gao, Yan.,Long, Xiao-hua.,Zhang, Zhi-yong.,Guo, Jun-yao.,...­an@jaas.ac.cn.(2014).Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community.CLEAN-SOIL AIR WATER,42(5),664-673.
MLA Yi, Neng,et al."Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community".CLEAN-SOIL AIR WATER 42.5(2014):664-673.
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