| Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker | |
Cui, Yulin1,2 ; Qin, Song1; Jiang, Peng2; Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai, Shandong, Peoples R China. sqin@yic.ac.cn
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| 发表期刊 | PLOS ONE
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| ISSN | 1932-6203 |
| 2014-06-09 | |
| 卷号 | 9期号:6页码:e98607 |
| 关键词 | Green Fluorescent Protein Plastid Transformation Higher-plants Transgenic Chloroplasts Chlamydomonas-reinhardtii Herbicide Resistance Glutamine-synthetase Expression Bombardment Biotechnology |
| DOI | 10.1371/journal.pone.0098607 |
| 产权排序 | [Cui, Yulin; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai, Shandong, Peoples R China; [Cui, Yulin; Jiang, Peng] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | The objective of this research was to establish a chloroplast transformation technique for Platymonas (Tetraselmis) subcordiformis. Employing the gfp gene as a reporter and the bar gene as a selectable marker, transformation vectors of P. subcordiformis chloroplast were constructed with endogenous fragments rrn16S-trnl (left) and trnA-rrn23S (right) as a recombination site of the chloroplast genome. The plasmids were transferred into P. subcordiformis via particle bombardment. Confocal laser scanning microscopy indicated that the green fluorescence protein was localized in the chloroplast of P. subcordiformis, confirming the activity of the Chlamydomonas reinhardtii promoter. Cells transformed with the bar gene were selected using the herbicide Basta. Resistant colonies were analyzed by PCR and Southern blotting, and the results indicated that the bar gene was successfully integrated into the chloroplast genome via homologous recombination. The technique will improve genetic engineering of this alga. |
| 文章类型 | Article |
| 资助机构 | Ocean Public Welfare Scientific Research Project, State Oceanic Administration of China 201205027 ; National Natural Science Foundation of China 41176144 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | GREEN FLUORESCENT PROTEIN ; PLASTID TRANSFORMATION ; HIGHER-PLANTS ; TRANSGENIC CHLOROPLASTS ; CHLAMYDOMONAS-REINHARDTII ; HERBICIDE RESISTANCE ; GLUTAMINE-SYNTHETASE ; EXPRESSION ; BOMBARDMENT ; BIOTECHNOLOGY |
| 研究领域[WOS] | Science & Technology |
| WOS记录号 | WOS:000337165600008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8547 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai, Shandong, Peoples R China. sqin@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Yulin,Qin, Song,Jiang, Peng,et al. Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker[J]. PLOS ONE,2014,9(6):e98607. |
| APA | Cui, Yulin,Qin, Song,Jiang, Peng,&Qin, S .(2014).Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker.PLOS ONE,9(6),e98607. |
| MLA | Cui, Yulin,et al."Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker".PLOS ONE 9.6(2014):e98607. |
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| Chloroplast Transfor（999KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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