| Reduced glutamine synthetase activity plays a role in control of photosynthetic responses to high light in barley leaves | |
| Brestic, Marian1; Zivcak, Marek1; Olsovska, Katarina1; Shao, Hong-Bo2,3,4; Kalaji, Hazem M.5; Allakhverdiev, Suleyman I.6,7; Brestic, M (reprint author), Slovak Univ Agr, Dept Plant Physiol, Tr A Hlinku 2, Nitra 94976, Slovakia. marian.brestic@uniag.sk; marek.zivcak@uniag.sk | |
| 发表期刊 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
 |
| ISSN | 0981-9428 |
| 2014-08-01 | |
| 卷号 | 81期号:SI页码:74-83 |
| 关键词 | Barley Glutamine Synthetase Photoinhibition Photorespiration Chlorophyll Fluorescence Non-photochemical Quenching |
| DOI | 10.1016/j.plaphy.2014.01.002 |
| 产权排序 | [Brestic, Marian; Zivcak, Marek; Olsovska, Katarina] Slovak Univ Agr, Dept Plant Physiol, Nitra 94976, Slovakia; [Shao, Hong-Bo] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Beijing 100864, Peoples R China; [Shao, Hong-Bo] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Beijing 100864, Peoples R China; [Shao, Hong-Bo] Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China; [Kalaji, Hazem M.] Warsaw Agr Univ SGGW, Fac Agr & Biol, Dept Plant Physiol, PL-02776 Warsaw, Poland; [Allakhverdiev, Suleyman I.] Russian Acad Sci, Inst Plant Physiol, Moscow 127276, Russia; [Allakhverdiev, Suleyman I.] Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142290, Moscow Region, Russia |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | The chloroplastic glutamine synthetase (GS, EC 6.3.1.2) activity was previously shown to be the limiting step of photorespiratory pathway. In our experiment, we examined the photosynthetic high-light responses of the GS2-mutant of barley (Hordeum vulgare L.) with reduced GS activity, in comparison to wild type (WT). The biophysical methods based on slow and fast chlorophyll fluorescence induction, P700 absorbance, and gas exchange measurements were employed. Despite the GS2 plants had high basal fluorescence (F-0) and low maximum quantum yield (F-v/F-m), the CO2 assimilation rate, the PSII and PSI actual quantum yields were normal. On the other hand, in high light conditions the GS2 had much higher non-photochemical quenching (NPQ), caused both by enhanced capacity of energy-dependent quenching and disconnection of PSII antennae from reaction centers (RC). GS2 leaves also maintained the PSII redox poise (Q((A) over bar)/Q(A) total) at very low level; probably this was reason why the observed photoinhibitory damage was not significantly above WT. The analysis of fast chlorophyll fluorescence induction uncovered in GS2 leaves substantially lower RC to antenna ratio (RC/ABS), low PSII/PSI ratio (confirmed by P700 records) as well as low PSII excitonic connectivity. (C) 2014 Elsevier Masson SAS. All rights reserved.; The chloroplastic glutamine synthetase (GS, EC 6.3.1.2) activity was previously shown to be the limiting step of photorespiratory pathway. In our experiment, we examined the photosynthetic high-light responses of the GS2-mutant of barley (Hordeum vulgare L.) with reduced GS activity, in comparison to wild type (WT). The biophysical methods based on slow and fast chlorophyll fluorescence induction, P700 absorbance, and gas exchange measurements were employed. Despite the GS2 plants had high basal fluorescence (F-0) and low maximum quantum yield (F-v/F-m), the CO2 assimilation rate, the PSII and PSI actual quantum yields were normal. On the other hand, in high light conditions the GS2 had much higher non-photochemical quenching (NPQ), caused both by enhanced capacity of energy-dependent quenching and disconnection of PSII antennae from reaction centers (RC). GS2 leaves also maintained the PSII redox poise (Q((A) over bar)/Q(A) total) at very low level; probably this was reason why the observed photoinhibitory damage was not significantly above WT. The analysis of fast chlorophyll fluorescence induction uncovered in GS2 leaves substantially lower RC to antenna ratio (RC/ABS), low PSII/PSI ratio (confirmed by P700 records) as well as low PSII excitonic connectivity. (C) 2014 Elsevier Masson SAS. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Plant Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | FLUORESCENCE RISE OJIP ; CYCLIC ELECTRON FLOW ; TRITICUM-AESTIVUM L ; PHOTOSYSTEM-II ; DROUGHT STRESS ; CHLOROPHYLL FLUORESCENCE ; PHOTORESPIRATORY METABOLISM ; EXCITONIC CONNECTIVITY ; CARBOXYLASE-OXYGENASE ; ENVIRONMENTAL-STRESS |
| 研究领域[WOS] | Plant Sciences |
| WOS记录号 | WOS:000340321400010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8540 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Brestic, M (reprint author), Slovak Univ Agr, Dept Plant Physiol, Tr A Hlinku 2, Nitra 94976, Slovakia. marian.brestic@uniag.sk; marek.zivcak@uniag.sk |
| 作者单位 | 1.Slovak Univ Agr, Dept Plant Physiol, Nitra 94976, Slovakia 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Beijing 100864, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Beijing 100864, Peoples R China 4.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China 5.Warsaw Agr Univ SGGW, Fac Agr & Biol, Dept Plant Physiol, PL-02776 Warsaw, Poland 6.Russian Acad Sci, Inst Plant Physiol, Moscow 127276, Russia 7.Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142290, Moscow Region, Russia |
| 推荐引用方式 GB/T 7714 | Brestic, Marian,Zivcak, Marek,Olsovska, Katarina,et al. Reduced glutamine synthetase activity plays a role in control of photosynthetic responses to high light in barley leaves[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2014,81(SI):74-83. |
| APA | Brestic, Marian.,Zivcak, Marek.,Olsovska, Katarina.,Shao, Hong-Bo.,Kalaji, Hazem M..,...&marek.zivcak@uniag.sk.(2014).Reduced glutamine synthetase activity plays a role in control of photosynthetic responses to high light in barley leaves.PLANT PHYSIOLOGY AND BIOCHEMISTRY,81(SI),74-83. |
| MLA | Brestic, Marian,et al."Reduced glutamine synthetase activity plays a role in control of photosynthetic responses to high light in barley leaves".PLANT PHYSIOLOGY AND BIOCHEMISTRY 81.SI(2014):74-83. |
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