Reconstructed metagenomes reveal changes of microbial functional profiling during PAHs degradation along a rice (Oryza sativa) rhizosphere gradient
Ma, B.; Lyu, X. -F.; Zha, T.; Gong, J.; He, Y.; Xu, J. -M.; Xu, JM (reprint author), Zhejiang Univ, Zhejiang Prov Key Lab Subtrop Soil & Plant Nutr, Inst Soil & Water Resources & Environm Sci, Hangzhou 310003, Zhejiang, Peoples R China. jmxu@zju.edu.cn
发表期刊JOURNAL OF APPLIED MICROBIOLOGY
ISSN1364-5072
2015-04
卷号118期号:4页码:890-900
关键词Microbial Community Phytoremediation Polycyclic Aromatic Hydrocarbons Reconstructed Metagenome Rhizosphere
DOI10.1111/jam.12756
产权排序[Ma, B.; Zha, T.; He, Y.; Xu, J. -M.] Zhejiang Univ, Zhejiang Prov Key Lab Subtrop Soil & Plant Nutr, Inst Soil & Water Resources & Environm Sci, Hangzhou 310003, Zhejiang, Peoples R China; [Ma, B.; Lyu, X. -F.; Gong, J.] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Microbial Ecol & Matter Cycles, Yantai, Peoples R China
作者部门海岸带生物学与生物资源利用所重点实验室
英文摘要AimsThe response of microbial metagenome to polycyclic aromatic hydrocarbons (PAHs) degradation in the rice rhizosphere remains poorly understood. We investigated the spatial and temporal variations of microbial communities and reconstructed metagenomes along the rice rhizosphere gradient during PAHs degradation. Methods and ResultsThe experiment was performed in rhizoboxes, in which the rhizosphere region was divided into five 1-mm thick layers. Based on denaturant gradient gel electrophoresis profiling and sequencing of bacterial and archaeal 16S rRNA genes, predicted metagenomes were reconstructed. The microbial communities in the rice rhizosphere were influenced by the PAHs concentration and distance from the root surface during PAHs degradation. Correlation network analysis showed that archaea played an important role in PAHs degradation. Predicted metagenomes can be clustered into two groups with high and low PAHs degrading potential, respectively. The relative abundance of genes for defense mechanisms, replication, recombination and reparation was significantly higher in samples with high PAHs degrading potentials. The relative abundance of the dioxygenase gene was greater near the root surface of the rice. However, the abundance of aldolase and dehydrogenase was constant in rhizosphere soils at different distances from the root surface. ConclusionsDistance from root surface and PAH concentrations affected the microbial communities and metagenomes in rice rhizosphere. The abundance of dioxygenase genes relating to PAH degradation in metagenomes mirrored the PAH degradation potential in rice rhizosphere. Significance and Impact of the StudyOur findings suggested that the predicted metagenomes reconstructed from 16S rRNA marker gene sequences provide further insights into the spatial variation and dynamics of microbial functioning that occur during bioremediation.
文章类型期刊论文
资助机构Biotechnology & Applied Microbiology ; Microbiology
收录类别SCI
语种英语
关键词[WOS]POLYCYCLIC AROMATIC-HYDROCARBONS ; ANAEROBIC DEGRADATION ; BACTERIAL COMMUNITIES ; GENE-SEQUENCES ; SOIL ; BIODEGRADATION ; PENTACHLOROPHENOL ; DIVERSITY ; SEDIMENT ; IMPACT
研究领域[WOS]National Natural Science Foundation of China [41090284, 41301333]
WOS记录号WOS:000351392200012
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/8507
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Xu, JM (reprint author), Zhejiang Univ, Zhejiang Prov Key Lab Subtrop Soil & Plant Nutr, Inst Soil & Water Resources & Environm Sci, Hangzhou 310003, Zhejiang, Peoples R China. jmxu@zju.edu.cn
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Ma, B.,Lyu, X. -F.,Zha, T.,et al. Reconstructed metagenomes reveal changes of microbial functional profiling during PAHs degradation along a rice (Oryza sativa) rhizosphere gradient[J]. JOURNAL OF APPLIED MICROBIOLOGY,2015,118(4):890-900.
APA Ma, B..,Lyu, X. -F..,Zha, T..,Gong, J..,He, Y..,...&Xu, JM .(2015).Reconstructed metagenomes reveal changes of microbial functional profiling during PAHs degradation along a rice (Oryza sativa) rhizosphere gradient.JOURNAL OF APPLIED MICROBIOLOGY,118(4),890-900.
MLA Ma, B.,et al."Reconstructed metagenomes reveal changes of microbial functional profiling during PAHs degradation along a rice (Oryza sativa) rhizosphere gradient".JOURNAL OF APPLIED MICROBIOLOGY 118.4(2015):890-900.
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