The invention relates to a non-hormone type feed additive containing organic selenium polysaccharide and preparation method thereof, belonging to the feed industry field. The preparation method comprises : adding selenium powder into concentrated nitric acid, diluting by water to obtain selenious acid solution which is called as solution I; adding the prepared solution I into sodium chloride solution dissolved with algal polysaccharides; reacting under 30-80 degrees centigrade for 12-20 hours; mixing the reaction residue- organic selenium polysaccharide and algal polysaccharides with filling material according to the weight percentages of 0.05%-0.5% of organic selenium polysaccharide, 0.06%-1.6% of algal polysaccharides and balanced amount of filling material; drying, grinding and obtaining the feed additive containing organic selenium polysaccharide. The feed additive of the invention is green, has no residue or pollution, can substitute antibiotics; also has high production efficiency, short cycle, and high selenium utilization rate, is safe and reliable for animal body without side effect.
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