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题名:
Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis
作者: Cui, Hongli1,2; Yu, Xiaona3; Wang, Yan(王艳)1; Cui, Yulin1; Li, Xueqin4; Liu, Zhaopu3; Qin, Song1
刊名: JOURNAL OF APPLIED PHYCOLOGY
ISSN号: 0921-8971
出版日期: 2014-02-01
卷号: 26, 期号:1, 页码:91-103
关键词: Xanthophylls biosynthesis ; Carotenoid hydroxylase ; Haematococcus pluvialis ; Gene cloning ; High light
学科分类: Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
产权排序: [Cui, Hongli; Wang, Yan; Cui, Yulin; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; [Cui, Hongli] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yu, Xiaona; Liu, Zhaopu] Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China; [Li, Xueqin] Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China
通讯作者: Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China. sea@njau.edu.cn ; sqin@yic.ac.cn
中文摘要: A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis.
英文摘要: A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis.
研究领域[WOS]: Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]: CHLORELLA-ZOFINGIENSIS CHLOROPHYTA ; ASTAXANTHIN BIOSYNTHETIC-PATHWAY ; FUNCTIONAL-ANALYSIS ; BETA-RING ; CLUSTAL-W ; ARABIDOPSIS ; ACCUMULATION ; LUTEIN ; PLANTS ; LIGHT
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000330969800012
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内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/7016
Appears in Collections:海岸带生物学与生物资源利用所重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China
4.Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China

Recommended Citation:
Cui, Hongli,Yu, Xiaona,Wang, Yan,et al. Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis[J]. JOURNAL OF APPLIED PHYCOLOGY,2014,26(1):91-103.
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