Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis
Cui, Hongli1,2; Yu, Xiaona3; Wang, Yan1; Cui, Yulin1; Li, Xueqin4; Liu, Zhaopu3; Qin, Song1; Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China. sea@njau.edu.cn; sqin@yic.ac.cn
2014-02-01
发表期刊JOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
卷号26期号:1页码:91-103
产权排序[Cui, Hongli; Wang, Yan; Cui, Yulin; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; [Cui, Hongli] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yu, Xiaona; Liu, Zhaopu] Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China; [Li, Xueqin] Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China
摘要A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis.; A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis.
关键词Xanthophylls Biosynthesis Carotenoid Hydroxylase Haematococcus Pluvialis Gene Cloning High Light
学科领域Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
项目资助者National Natural Science Foundation of China [40876082]; International Innovation Partnership Program: Typical Environmental Process and Effects on Resources in Coastal Zone Area; Public Science and Technology Research Funds Projects of the Ocean [200905021, 201205027]; Natural Science Foundation of Shandong Province [ZR2012DQ015]; Guangdong province comprehensive strategic cooperation project of the Chinese Academy of Sciences [2011A090100040]; Science and Technology Planning Project of Yantai, China [2010247]
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收录类别SCI
关键词[WOS]CHLORELLA-ZOFINGIENSIS CHLOROPHYTA ; ASTAXANTHIN BIOSYNTHETIC-PATHWAY ; FUNCTIONAL-ANALYSIS ; BETA-RING ; CLUSTAL-W ; ARABIDOPSIS ; ACCUMULATION ; LUTEIN ; PLANTS ; LIGHT
文章类型Article
语种英语
资助项目海岸带生物学与生物资源利用所重点实验室
WOS研究方向Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号WOS:000330969800012
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/7016
专题海岸带生物学与生物资源利用所重点实验室
通讯作者Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China. sea@njau.edu.cn; sqin@yic.ac.cn
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China
4.Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
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Cui, Hongli,Yu, Xiaona,Wang, Yan,et al. Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis[J]. JOURNAL OF APPLIED PHYCOLOGY,2014,26(1):91-103.
APA Cui, Hongli.,Yu, Xiaona.,Wang, Yan.,Cui, Yulin.,Li, Xueqin.,...&sqin@yic.ac.cn.(2014).Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis.JOURNAL OF APPLIED PHYCOLOGY,26(1),91-103.
MLA Cui, Hongli,et al."Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis".JOURNAL OF APPLIED PHYCOLOGY 26.1(2014):91-103.
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