Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis
Cui, Hongli1,2; Yu, Xiaona3; Wang, Yan1; Cui, Yulin1; Li, Xueqin4; Liu, Zhaopu3; Qin, Song1; Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China. sea@njau.edu.cn; sqin@yic.ac.cn
发表期刊JOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
2014-02-01
卷号26期号:1页码:91-103
关键词Xanthophylls Biosynthesis Carotenoid Hydroxylase Haematococcus Pluvialis Gene Cloning High Light
产权排序[Cui, Hongli; Wang, Yan; Cui, Yulin; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; [Cui, Hongli] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yu, Xiaona; Liu, Zhaopu] Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China; [Li, Xueqin] Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China
英文摘要A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis.; A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis.
文章类型Article
资助机构National Natural Science Foundation of China [40876082]; International Innovation Partnership Program: Typical Environmental Process and Effects on Resources in Coastal Zone Area; Public Science and Technology Research Funds Projects of the Ocean [200905021, 201205027]; Natural Science Foundation of Shandong Province [ZR2012DQ015]; Guangdong province comprehensive strategic cooperation project of the Chinese Academy of Sciences [2011A090100040]; Science and Technology Planning Project of Yantai, China [2010247]
收录类别SCI
语种英语
关键词[WOS]CHLORELLA-ZOFINGIENSIS CHLOROPHYTA ; ASTAXANTHIN BIOSYNTHETIC-PATHWAY ; FUNCTIONAL-ANALYSIS ; BETA-RING ; CLUSTAL-W ; ARABIDOPSIS ; ACCUMULATION ; LUTEIN ; PLANTS ; LIGHT
研究领域[WOS]Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号WOS:000330969800012
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/7016
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China. sea@njau.edu.cn; sqin@yic.ac.cn
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China
4.Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China
推荐引用方式
GB/T 7714
Cui, Hongli,Yu, Xiaona,Wang, Yan,et al. Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis[J]. JOURNAL OF APPLIED PHYCOLOGY,2014,26(1):91-103.
APA Cui, Hongli.,Yu, Xiaona.,Wang, Yan.,Cui, Yulin.,Li, Xueqin.,...&sqin@yic.ac.cn.(2014).Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis.JOURNAL OF APPLIED PHYCOLOGY,26(1),91-103.
MLA Cui, Hongli,et al."Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis".JOURNAL OF APPLIED PHYCOLOGY 26.1(2014):91-103.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Gene cloning and exp(1210KB) 开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cui, Hongli]的文章
[Yu, Xiaona]的文章
[Wang, Yan]的文章
百度学术
百度学术中相似的文章
[Cui, Hongli]的文章
[Yu, Xiaona]的文章
[Wang, Yan]的文章
必应学术
必应学术中相似的文章
[Cui, Hongli]的文章
[Yu, Xiaona]的文章
[Wang, Yan]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。