Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Tetra(p-tolyl)borate-Functionalized Solvent Polymeric Membrane: A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics | |
Wang, Xuewei1,2; Qin, Wei1
| |
| 发表期刊 | CHEMISTRY-A EUROPEAN JOURNAL
 |
| ISSN | 0947-6539 |
| 2013-07-22 | |
| 卷号 | 19期号:30页码:9979-9986 |
| 关键词 | Biosensors Intermediate-sensitive Membranes Peroxidase Peroxidase Mimetics Potentiometry |
| 产权排序 | [Wang, Xuewei; Qin, Wei] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Wang, Xuewei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Qin, W (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn |
| 英文摘要 | The determination of peroxidase activities is the basis for enzyme-labeled bioaffinity assays, peroxidase-mimicking DNAzymes- and nanoparticles-based assays, and characterization of the catalytic functions of peroxidase mimetics. Here, a facile, sensitive, and cost-effective solvent polymeric membrane-based peroxidase detection platform is described that utilizes reaction intermediates with different pK(a) values from those of substrates and final products. Several key but long-debated intermediates in the peroxidative oxidation of o-phenylenediamine (o-PD) have been identified and their charge states have been estimated. By using a solvent polymeric membrane functionalized by an appropriate substituted tetraphenylborate as a receptor, those cationic intermediates could be transferred into the membrane from the aqueous phase to induce a large cationic potential response. Thus, the potentiometric indication of the o-PD oxidation catalyzed by peroxidase or its mimetics can be fulfilled. Horseradish peroxidase has been detected with a detection limit at least two orders of magnitude lower than those obtained by spectrophotometric techniques and traditional membrane-based methods. As an example of peroxidase mimetics, G-quadruplex DNAzymes were probed by the intermediate-sensitive membrane and a label-free thrombin detection protocol was developed based on the catalytic activity of the thrombin-binding G-quadruplex aptamer.; The determination of peroxidase activities is the basis for enzyme-labeled bioaffinity assays, peroxidase-mimicking DNAzymes- and nanoparticles-based assays, and characterization of the catalytic functions of peroxidase mimetics. Here, a facile, sensitive, and cost-effective solvent polymeric membrane-based peroxidase detection platform is described that utilizes reaction intermediates with different pK(a) values from those of substrates and final products. Several key but long-debated intermediates in the peroxidative oxidation of o-phenylenediamine (o-PD) have been identified and their charge states have been estimated. By using a solvent polymeric membrane functionalized by an appropriate substituted tetraphenylborate as a receptor, those cationic intermediates could be transferred into the membrane from the aqueous phase to induce a large cationic potential response. Thus, the potentiometric indication of the o-PD oxidation catalyzed by peroxidase or its mimetics can be fulfilled. Horseradish peroxidase has been detected with a detection limit at least two orders of magnitude lower than those obtained by spectrophotometric techniques and traditional membrane-based methods. As an example of peroxidase mimetics, G-quadruplex DNAzymes were probed by the intermediate-sensitive membrane and a label-free thrombin detection protocol was developed based on the catalytic activity of the thrombin-binding G-quadruplex aptamer. |
| 文章类型 | Article |
| 资助机构 | Instrument Developing Project of the Chinese Academy of Sciences [YZ201161]; National Natural Science Foundation of China [41176081]; Taishan Scholar Program of Shandong Province [TS20081159] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | QUADRUPLEX-BASED DNAZYME ; ION-SELECTIVE ELECTRODE ; BENZOQUINONE DI-IMINES ; HORSERADISH-PEROXIDASE ; O-PHENYLENEDIAMINE ; COLORIMETRIC DETECTION ; ELECTROCHEMICAL ASSAY ; CATALYZED OXIDATION ; ENZYME-IMMUNOASSAY ; HYDROGEN-PEROXIDE |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000321766400036 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6973 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xuewei,Qin, Wei. Tetra(p-tolyl)borate-Functionalized Solvent Polymeric Membrane: A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics[J]. CHEMISTRY-A EUROPEAN JOURNAL,2013,19(30):9979-9986. |
| APA | Wang, Xuewei,&Qin, Wei.(2013).Tetra(p-tolyl)borate-Functionalized Solvent Polymeric Membrane: A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics.CHEMISTRY-A EUROPEAN JOURNAL,19(30),9979-9986. |
| MLA | Wang, Xuewei,et al."Tetra(p-tolyl)borate-Functionalized Solvent Polymeric Membrane: A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics".CHEMISTRY-A EUROPEAN JOURNAL 19.30(2013):9979-9986. |
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