Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone | |
| 其他题名 | The heavy metal partition in size-fractions of the fine particles in agricultural soils contaminated by waste water and smelter dust.pdf |
Yin, Kun1,2; Zhang, Weiwei1; Chen, Lingxin1
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| 发表期刊 | BIOSENSORS & BIOELECTRONICS
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| ISSN | 0956-5663 |
| 2014-01-15 | |
| 卷号 | 51页码:90-96 |
| 关键词 | Furazolidone Pseudomonas Aeruginosa Strain Pa1 Pyoverdine Fluorescent Detection |
| 产权排序 | [Yin, Kun; Zhang, Weiwei; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Yin, Kun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China. lxchen@yic.ac.cn |
| 英文摘要 | Methods for the rapid and sensitive detection of furazolidone, a pesticide used for the treatment of infections of animals and human beings, have been urgently recommended for its large residual, strong carcinogenicity and genotoxicity in the environment. In this study, a method for the detection of furazolidone based on the rapid fluorescence quenching of pyoverdine by furazolidone was developed. Pyoverdine secreted by a Pseudomonas aeruginosa strain PA1 was purified through affinity chromatography and its fluorescent property was characterized. The fluorescence of pyoverdine could be quenched by furazolidone with specificity, and based on this phenomenon a fluorescent method for furazolidone detection was established. Fluorescence of pyoverdine was quenched by furazolidone probably due to the electron transfer from pyoverdine to furazolidone. The optimal pH for the detection was 7.2 in 50 mM 3-(N-Morpholino) propanesulfonic acid solution, and the whole detection process could be completed within a few seconds. The linear range of the detection was 2-160 mu M and the limit of detection (LOD) was 0.5 mu M. This study developed a novel fluorescent method for furazolidone detection, and the rapid and specific fluorescent biosensor can be potentially applied for furazolidone detection in the aquatic samples. (C) 2013 Elsevier B.V. All rights reserved.; Methods for the rapid and sensitive detection of furazolidone, a pesticide used for the treatment of infections of animals and human beings, have been urgently recommended for its large residual, strong carcinogenicity and genotoxicity in the environment. In this study, a method for the detection of furazolidone based on the rapid fluorescence quenching of pyoverdine by furazolidone was developed. Pyoverdine secreted by a Pseudomonas aeruginosa strain PA1 was purified through affinity chromatography and its fluorescent property was characterized. The fluorescence of pyoverdine could be quenched by furazolidone with specificity, and based on this phenomenon a fluorescent method for furazolidone detection was established. Fluorescence of pyoverdine was quenched by furazolidone probably due to the electron transfer from pyoverdine to furazolidone. The optimal pH for the detection was 7.2 in 50 mM 3-(N-Morpholino) propanesulfonic acid solution, and the whole detection process could be completed within a few seconds. The linear range of the detection was 2-160 mu M and the limit of detection (LOD) was 0.5 mu M. This study developed a novel fluorescent method for furazolidone detection, and the rapid and specific fluorescent biosensor can be potentially applied for furazolidone detection in the aquatic samples. (C) 2013 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences [KZCX2-EW-206]; National Natural Science Foundation of China [31200041, 21275158]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry; 100 Talents Program of Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ORGANOPHOSPHATE PESTICIDES ; MICROBIAL BIOSENSOR ; MASS-SPECTROMETRY ; QUANTUM DOTS ; 3-AMINO-2-OXAZOLIDINONE ; CHROMATOGRAPHY ; TISSUES ; TRINITROTOLUENE ; NITROFURAZONE ; BIOSYNTHESIS |
| 研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS记录号 | WOS:000326905500013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6911 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Kun,Zhang, Weiwei,Chen, Lingxin. Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone[J]. BIOSENSORS & BIOELECTRONICS,2014,51:90-96. |
| APA | Yin, Kun,Zhang, Weiwei,&Chen, Lingxin.(2014).Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone.BIOSENSORS & BIOELECTRONICS,51,90-96. |
| MLA | Yin, Kun,et al."Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone".BIOSENSORS & BIOELECTRONICS 51(2014):90-96. |
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