FITC functionalized magnetic core-shell Fe3O4/Ag hybrid nanoparticle for selective determination of molecular biothiols
Chen, Ling1,5; Li, Jinhua1; Wang, Shasha1,5; Lu, Wenhui1; Wu, Aiguo2,3; Choo, Jaebum4; Chen, Lingxin1
2014-03-01
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
卷号193页码:857-863
产权排序[Chen, Ling; Li, Jinhua; Wang, Shasha; Lu, Wenhui; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wu, Aiguo] Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Key Lab Magnet Mat & Devices, Ningbo 315201, Peoples R China; [Wu, Aiguo] Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Div Funct Mat & Nanodevices, Ningbo 315201, Peoples R China; [Choo, Jaebum] Chinese Acad Sci, Dept Bionano Technol, Ningbo 315201, Peoples R China; [Chen, Ling; Wang, Shasha] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
摘要A sensing strategy for chromogenic detection of molecular biothiols has been proposed based on fluorescein isothiocyanate (FITC) functionalized magnetic core-shell Fe3O4/Ag hybrid nanoparticles. Ag coated magnetic Fe3O4 nanoparticles were initially synthetized. FITC was subsequently conjugated on the surface of core-shell nanoparticles by Ag-SCN linkage and then the fluorescence of FITC was quenched. Upon addition of molecular biothiols, since the Ag S bond is stronger than Ag-SCN, a place-displacement between thiols and FITC would occur and thereby the fluorescence of FITC would recover. Thus, a fluorescence "off-on" probe was attained by virtue of biothiols, so after magnetic separation, the fluorescence signal change of FITC in clear solution could be employed for quantitative determination of typical molecular biothiols such as glutathione (GSH) and cysteine (Cys). High sensitivity was obtained with the detection limits of 10 nM and 20 nM for GSH and Cys, respectively. As well as, the assay strategy presented excellent selectivity toward molecular biothiols against other amino acids. Furthermore, confocal imaging was achieved in living cells, indicating that this fluorescent probe is potentially applicable for imaging molecular thiols in biological systems. (C) 2013 Elsevier B.V. All rights reserved.; A sensing strategy for chromogenic detection of molecular biothiols has been proposed based on fluorescein isothiocyanate (FITC) functionalized magnetic core-shell Fe3O4/Ag hybrid nanoparticles. Ag coated magnetic Fe3O4 nanoparticles were initially synthetized. FITC was subsequently conjugated on the surface of core-shell nanoparticles by Ag-SCN linkage and then the fluorescence of FITC was quenched. Upon addition of molecular biothiols, since the Ag S bond is stronger than Ag-SCN, a place-displacement between thiols and FITC would occur and thereby the fluorescence of FITC would recover. Thus, a fluorescence "off-on" probe was attained by virtue of biothiols, so after magnetic separation, the fluorescence signal change of FITC in clear solution could be employed for quantitative determination of typical molecular biothiols such as glutathione (GSH) and cysteine (Cys). High sensitivity was obtained with the detection limits of 10 nM and 20 nM for GSH and Cys, respectively. As well as, the assay strategy presented excellent selectivity toward molecular biothiols against other amino acids. Furthermore, confocal imaging was achieved in living cells, indicating that this fluorescent probe is potentially applicable for imaging molecular thiols in biological systems. (C) 2013 Elsevier B.V. All rights reserved.
通讯作者Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
关键词Molecular Biothiols Chromogenic Detection Silver Coated Magnetic Nanoparticles Fluorescein Isothiocyanate Living Cells
学科领域Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
项目资助者Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry; Chinese Academy of Sciences [KZCX2-EW-206]; National Research Foundation of Korea [R11-2008-0061852, K20904000004-12A0500-00410]; Program of Zhejiang Provincial Natural Science Foundation of China [R5110230]; 100 Talents Program of the Chinese Academy of Sciences
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收录类别SCI
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; QUINONE MODIFIED ELECTRODE ; CAPILLARY-ELECTROPHORESIS ; ELECTROCHEMICAL DETECTION ; ALZHEIMERS-DISEASE ; FLUORESCENT-PROBE ; LIVING CELLS ; HUMAN PLASMA ; THIOLS ; CYSTEINE
文章类型Article
语种英语
资助项目中国科学院海岸带环境过程与生态修复重点实验室
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000330113600119
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文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6902
专题中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Key Lab Magnet Mat & Devices, Ningbo 315201, Peoples R China
3.Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Div Funct Mat & Nanodevices, Ningbo 315201, Peoples R China
4.Chinese Acad Sci, Dept Bionano Technol, Ningbo 315201, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
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Chen, Ling,Li, Jinhua,Wang, Shasha,et al. FITC functionalized magnetic core-shell Fe3O4/Ag hybrid nanoparticle for selective determination of molecular biothiols[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,193:857-863.
APA Chen, Ling.,Li, Jinhua.,Wang, Shasha.,Lu, Wenhui.,Wu, Aiguo.,...&Chen, Lingxin.(2014).FITC functionalized magnetic core-shell Fe3O4/Ag hybrid nanoparticle for selective determination of molecular biothiols.SENSORS AND ACTUATORS B-CHEMICAL,193,857-863.
MLA Chen, Ling,et al."FITC functionalized magnetic core-shell Fe3O4/Ag hybrid nanoparticle for selective determination of molecular biothiols".SENSORS AND ACTUATORS B-CHEMICAL 193(2014):857-863.
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