Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays | |
Zhang, Weiwei1,2; Niu, Zongliang1,2; Yin, Kun1,2,3; Liu, Ping1,2; Chen, Lingxin1,2,4
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| 发表期刊 | ANNALS OF MICROBIOLOGY
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| ISSN | 1590-4261 |
| 2013-06-01 | |
| 卷号 | 63期号:2页码:683-689 |
| 关键词 | Proteus Mirabilis Urer Polymerase Chain Reaction (Pcr) Real-time Pcr |
| 产权排序 | [Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Liu, Ping; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Liu, Ping; Chen, Lingxin] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Yin, Kun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China. lxchen@yic.ac.cn |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Proteus mirabilis is an opportunistic pathogen that can cause urinary tract infection in human beings. The accurate and rapid identification and quantification of P. mirabilis is necessary for early treatment. In this study, a pair of specific primers according to the conserved ureR sequence of P. mirabilis was designed and novel systems which consisted of a polymerase chain reaction (PCR) and a real-time PCR to identify and quantify P. mirabilis were developed. For the qualitative identification by ordinary PCR, a 225-bp DNA product was amplified from P. mirabilis and separated on an agarose gel. The corresponding DNA product is present in three P. mirabilis strains isolated from different geographical locations, but is absent in 20 strains representing 18 different species, including the ureR homolog contained Providencia stuartii and Escherichia coli strains, the other common pathogens Klebsiella sp., Edwarsiella sp., Vibrio sp., Enterobacter sp., and Escherichia sp., and other environmental bacteria Pseudomonas sp. and Acinetobacter sp. Proteus mirabilis at concentrations higher than 1.0 x 10(3) CFU ml(-1) was detectable by ordinary PCR; P. mirabilis at concentrations higher than 10 CFU ml(-1) was quantified by real-time PCR. The specific, sensitive and time-efficient PCR methods were demonstrated to be applicable to rapid identification and quantification of P. mirabilis.; Proteus mirabilis is an opportunistic pathogen that can cause urinary tract infection in human beings. The accurate and rapid identification and quantification of P. mirabilis is necessary for early treatment. In this study, a pair of specific primers according to the conserved ureR sequence of P. mirabilis was designed and novel systems which consisted of a polymerase chain reaction (PCR) and a real-time PCR to identify and quantify P. mirabilis were developed. For the qualitative identification by ordinary PCR, a 225-bp DNA product was amplified from P. mirabilis and separated on an agarose gel. The corresponding DNA product is present in three P. mirabilis strains isolated from different geographical locations, but is absent in 20 strains representing 18 different species, including the ureR homolog contained Providencia stuartii and Escherichia coli strains, the other common pathogens Klebsiella sp., Edwarsiella sp., Vibrio sp., Enterobacter sp., and Escherichia sp., and other environmental bacteria Pseudomonas sp. and Acinetobacter sp. Proteus mirabilis at concentrations higher than 1.0 x 10(3) CFU ml(-1) was detectable by ordinary PCR; P. mirabilis at concentrations higher than 10 CFU ml(-1) was quantified by real-time PCR. The specific, sensitive and time-efficient PCR methods were demonstrated to be applicable to rapid identification and quantification of P. mirabilis. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences [KZCX2-EW-206]; National Natural Science Foundation of China (NSFC) [20975089]; Department of Science and Technology of Yantai City of China [2010235]; Doctoral Foundation of Shandong Province [BS2011SW056]; 100 Talents Program of the Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | REAL-TIME PCR ; SYBR GREEN-I ; ANTIBIOTIC SUSCEPTIBILITY ; RAPID DETECTION ; VIBRIO-HARVEYI ; RIBOSOMAL-RNA ; URINARY-TRACT ; ENTEROBACTERIACEAE ; SAMPLES ; SPECIMENS |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| WOS记录号 | WOS:000319074600032 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6658 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Weiwei,Niu, Zongliang,Yin, Kun,et al. Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays[J]. ANNALS OF MICROBIOLOGY,2013,63(2):683-689. |
| APA | Zhang, Weiwei,Niu, Zongliang,Yin, Kun,Liu, Ping,&Chen, Lingxin.(2013).Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays.ANNALS OF MICROBIOLOGY,63(2),683-689. |
| MLA | Zhang, Weiwei,et al."Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays".ANNALS OF MICROBIOLOGY 63.2(2013):683-689. |
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