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| Polymeric Membrane Neutral Phenol-Sensitive Electrodes for Potentiometric G-Quadruplex/Hemin DNAzyme-Based Biosensing | |
Wang, Xuewei1,2; Ding, Zhaofeng3; Ren, Qingwei3; Qin, Wei1
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| 发表期刊 | ANALYTICAL CHEMISTRY
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| ISSN | 0003-2700 |
| 2013-02-05 | |
| 卷号 | 85期号:3页码:1945-1950 |
| 关键词 | Enzyme-immunoassay P-cresol Dna Derivatives Mechanism Oxidation |
| 产权排序 | [Wang, Xuewei; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Wang, Xuewei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Ding, Zhaofeng; Ren, Qingwei] Yantai Univ, Coll Chem & Chem Engn, Yantai 264003, Peoples R China |
| 通讯作者 | Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China. wqin@yic.ac.cn |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | The first potentiometric transducer for G-quadruplex/hemin DNAzyme-based biosensing has been developed by using potential responses of electrically neutral oligomeric phenols on polymeric membrane electrodes. In the presence of G-quadruplex/hemin DNAzyme and H2O2, monomeric phenols (e.g., phenol, methylphenols, and methoxyphenols) can be condensed into oligomeric phenols. Because both substrates and products are nonionic under optimal pH conditions, these reactions are traditionally not considered in designing potentiometric biosensing schemes. However, in this paper, the electrically neutral oligomeric phenols have been found to induce highly sensitive potential responses on quaternary ammonium salt-doped polymeric membrane electrodes owing to their high lipophilicities. In contrast, the potential responses to monomeric phenolic substrates are rather low. Thus, the G-quadruplex/hemin DNAzyme-catalyzed oxidative coupling of monomeric phenols can induce large potential signals, and the catalytic activities of DNAzymes can be probed. A comparison of potential responses induced by peroxidations of 13 monomeric phenols indicates that p-methoxyphenol is the most efficient substrate for potentiometric detection of G-quadruplex/hemin DNAzymes. Finally, two label-free and separation-free potentiometric DNA assay protocols based on the G-quadruplex/hemin DNAzyme have been developed with sensitivities higher than those of colorimetric and fluorometric methods. Coupled with other features such as reliable instrumentation, low cost, ease of miniaturization, and resistance to color and turbid interferences, the proposed polymeric membrane-based potentiometric sensor promises to be a competitive transducer for peroxidase-mimicking DNAzyme-involved biosensing.; The first potentiometric transducer for G-quadruplex/hemin DNAzyme-based biosensing has been developed by using potential responses of electrically neutral oligomeric phenols on polymeric membrane electrodes. In the presence of G-quadruplex/hemin DNAzyme and H2O2, monomeric phenols (e.g., phenol, methylphenols, and methoxyphenols) can be condensed into oligomeric phenols. Because both substrates and products are nonionic under optimal pH conditions, these reactions are traditionally not considered in designing potentiometric biosensing schemes. However, in this paper, the electrically neutral oligomeric phenols have been found to induce highly sensitive potential responses on quaternary ammonium salt-doped polymeric membrane electrodes owing to their high lipophilicities. In contrast, the potential responses to monomeric phenolic substrates are rather low. Thus, the G-quadruplex/hemin DNAzyme-catalyzed oxidative coupling of monomeric phenols can induce large potential signals, and the catalytic activities of DNAzymes can be probed. A comparison of potential responses induced by peroxidations of 13 monomeric phenols indicates that p-methoxyphenol is the most efficient substrate for potentiometric detection of G-quadruplex/hemin DNAzymes. Finally, two label-free and separation-free potentiometric DNA assay protocols based on the G-quadruplex/hemin DNAzyme have been developed with sensitivities higher than those of colorimetric and fluorometric methods. Coupled with other features such as reliable instrumentation, low cost, ease of miniaturization, and resistance to color and turbid interferences, the proposed polymeric membrane-based potentiometric sensor promises to be a competitive transducer for peroxidase-mimicking DNAzyme-involved biosensing. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences [YZ201161]; National Natural Science Foundation of China [41176081]; Taishan Scholar Program of Shandong Province [TS20081159] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ENZYME-IMMUNOASSAY ; P-CRESOL ; DNA ; DERIVATIVES ; MECHANISM ; OXIDATION |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000314676100099 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6648 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yantai Univ, Coll Chem & Chem Engn, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xuewei,Ding, Zhaofeng,Ren, Qingwei,et al. Polymeric Membrane Neutral Phenol-Sensitive Electrodes for Potentiometric G-Quadruplex/Hemin DNAzyme-Based Biosensing[J]. ANALYTICAL CHEMISTRY,2013,85(3):1945-1950. |
| APA | Wang, Xuewei,Ding, Zhaofeng,Ren, Qingwei,&Qin, Wei.(2013).Polymeric Membrane Neutral Phenol-Sensitive Electrodes for Potentiometric G-Quadruplex/Hemin DNAzyme-Based Biosensing.ANALYTICAL CHEMISTRY,85(3),1945-1950. |
| MLA | Wang, Xuewei,et al."Polymeric Membrane Neutral Phenol-Sensitive Electrodes for Potentiometric G-Quadruplex/Hemin DNAzyme-Based Biosensing".ANALYTICAL CHEMISTRY 85.3(2013):1945-1950. |
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