| Gene Regulation of Iron-Deficiency Responses Is Associated with Carbon Monoxide and Heme Oxydase 1 in Chlamydomonas reinhardtii | |
| Zhang Liping1,2; Shao Hongbo1,2,3; Long Xiaohua1; Liu Zhaopu1 | |
| 发表期刊 | PLOS ONE
 |
| ISSN | 1932-6203 |
| 2013-01-22 | |
| 卷号 | 8期号:1页码:e53835 |
| 关键词 | Phytochrome-chromophore Biosynthesis Nitric-oxide Metal Transporter Effector Molecules Chelate Reductase Arabidopsis Plants Accumulation Metabolism Expression |
| 产权排序 | [Zhang Liping; Shao Hongbo; Long Xiaohua; Liu Zhaopu] Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Key Lab Marine Biol, Nanjing, Jiangsu, Peoples R China; [Zhang Liping; Shao Hongbo] Chinese Acad Sci, Yantai Inst Costal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai, Peoples R China; [Shao Hongbo] Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao, Peoples R China |
| 通讯作者 | Shao, HB (reprint author), Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Key Lab Marine Biol, Nanjing, Jiangsu, Peoples R China. shaohongbochu@126.com ; longxiaohua@njau.edu.cn ; sea@njau.edu.cn |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Carbon monoxide (CO) as an endogenous gaseous molecule regulates a variety of biological processes in animals. However, CO regulating nutrient stress responses in green alga is largely unknown. On the other hand, heme oxydase (HO1 as a rate-limiting enzyme of the first step for heme degration and to catalyze heme into biliverdin (BV), which is concomitant with releasing of CO and ferrous ions, probably participates in the process of CO-regulating response to nutrient stress in green alga. In this paper, we described an observation that CO could regulate iron-homeostasis in iron-starving Chlamydomonas reinhardtii. Exogenous CO at 8 mu M was able to prevent the iron deficient-inducing chlorosis and improve chlorophyll accumulation. Expression pattern of FOX1, FTR1 and ferredoxin was up-regulated by CO exposure in iron-deficient mediam. treatment with external CO increasing iron accumulation in iron-deficient C. reinhardtii. Moreover, to get insights into the regulatory role of HO1, we constructed a transgenic alga overexpressing HO1 and HO1 knock-out mutants. The results show that there was no significant influence on chlorosis with HO1 overexpression of C. reinhardtii under iron-deficiency and the chlorophyll accumulation, and gene expression associated with iron deficiency of mutant were greatly improved. Otherwise, those results from HO1 knock-out mutants were opposite to HO1 overexpression mutants. Finally, CO exposure induced NO accumulation in cells. However, such an action could be blocked by NO scavenger cPTIO. These results indicate that CO/HO1 may play an important role in improving green algae adaptation to iron deficiency or cross-talking with NO under the iron deficiency.; Carbon monoxide (CO) as an endogenous gaseous molecule regulates a variety of biological processes in animals. However, CO regulating nutrient stress responses in green alga is largely unknown. On the other hand, heme oxydase (HO1 as a rate-limiting enzyme of the first step for heme degration and to catalyze heme into biliverdin (BV), which is concomitant with releasing of CO and ferrous ions, probably participates in the process of CO-regulating response to nutrient stress in green alga. In this paper, we described an observation that CO could regulate iron-homeostasis in iron-starving Chlamydomonas reinhardtii. Exogenous CO at 8 mu M was able to prevent the iron deficient-inducing chlorosis and improve chlorophyll accumulation. Expression pattern of FOX1, FTR1 and ferredoxin was up-regulated by CO exposure in iron-deficient mediam. treatment with external CO increasing iron accumulation in iron-deficient C. reinhardtii. Moreover, to get insights into the regulatory role of HO1, we constructed a transgenic alga overexpressing HO1 and HO1 knock-out mutants. The results show that there was no significant influence on chlorosis with HO1 overexpression of C. reinhardtii under iron-deficiency and the chlorophyll accumulation, and gene expression associated with iron deficiency of mutant were greatly improved. Otherwise, those results from HO1 knock-out mutants were opposite to HO1 overexpression mutants. Finally, CO exposure induced NO accumulation in cells. However, such an action could be blocked by NO scavenger cPTIO. These results indicate that CO/HO1 may play an important role in improving green algae adaptation to iron deficiency or cross-talking with NO under the iron deficiency. |
| 文章类型 | Article |
| 资助机构 | National Key Project of Scientific and Technical Supporting Programs [2011BAD13B09]; Jiangsu Provincial Key Project of Scientific and Technical Supporting Programs [BE2010305, BE2011368]; Fundamental Research Funds for the Central Universities [Y0201100249]; New Teachers' Fund for Doctor Stations, Ministry of Education [20100097120016]; Project of Special Fund for Public Welfare Industrial (Agriculture) Research of China [200903001-5]; National Natural Science Foundation of China [31201692]; One Hundred-Talent Plan of Chinese Academy of Sciences (CAS); CAS/SAFEA International Partnership Program for Creative Research Teams; Yantai Science & Technology Development Project [2011016, 2010245]; Yantai Double-hundred Talent Plan [XY-003-02]; 135 Plan Development Plan of YIC-CAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PHYTOCHROME-CHROMOPHORE BIOSYNTHESIS ; NITRIC-OXIDE ; METAL TRANSPORTER ; EFFECTOR MOLECULES ; CHELATE REDUCTASE ; ARABIDOPSIS ; PLANTS ; ACCUMULATION ; METABOLISM ; EXPRESSION |
| 研究领域[WOS] | Science & Technology - Other Topics |
| WOS记录号 | WOS:000314019100033 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6438 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Key Lab Marine Biol, Nanjing, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Costal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai, Peoples R China 3.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Liping,Shao Hongbo,Long Xiaohua,et al. Gene Regulation of Iron-Deficiency Responses Is Associated with Carbon Monoxide and Heme Oxydase 1 in Chlamydomonas reinhardtii[J]. PLOS ONE,2013,8(1):e53835. |
| APA | Zhang Liping,Shao Hongbo,Long Xiaohua,&Liu Zhaopu.(2013).Gene Regulation of Iron-Deficiency Responses Is Associated with Carbon Monoxide and Heme Oxydase 1 in Chlamydomonas reinhardtii.PLOS ONE,8(1),e53835. |
| MLA | Zhang Liping,et al."Gene Regulation of Iron-Deficiency Responses Is Associated with Carbon Monoxide and Heme Oxydase 1 in Chlamydomonas reinhardtii".PLOS ONE 8.1(2013):e53835. |
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