Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria
Shi Fei1,3; Li Nan1,2,3; Liu ShaoFang3,4; Qin Song1
发表期刊CHINESE SCIENCE BULLETIN
ISSN1001-6538
2012-09-01
卷号57期号:25页码:3270-3275
关键词Water Bloom Phytochrome Microcystis Aeruginosa Positive Selection Cyanobacteria Spectral Characteristic
产权排序[Shi Fei; Li Nan; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Li Nan] Korean Ocean Res & Dev Inst, Marine Biotechnol Res Ctr, Ansan 425600, South Korea; [Shi Fei; Li Nan; Liu ShaoFang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Liu ShaoFang] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
通讯作者Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.,sqin@yic.ac.cn
作者部门海岸带生物学与生物资源利用所重点实验室
英文摘要The phytochrome gene from Microcystis aeruginosa FACHB-912, isolated from algal blooms in Taihu Lake in China, was sequenced and found to code for a wavelength transition light receptor protein. The gene was composed of five distinct domains: a PAS fold domain, a GAF domain, a phytochrome domain, a His Kinase A (phosphoacceptor) domain, and a histidine kinase domain. Red-absorbing phytochrome, far-red-absorbing phytochrome, and photoconversion kinetics were monitored via the spectral characteristics of the protein. To detect positive selection, homologous sequences from cyanobacterium phytochrome genes were obtained from GenBank. Potential selective pressure was identified by phylogenetic analysis with maximum likelihood analyses. The frequency of the phytochrome gene being subjected to selective constraints suggests that positive selection is a potentially important mechanism that promotes the evolution of the cyanobacteria phytochrome gene. The sites identified in this study provide targets for further research on the structural-functional role of these residues, and on the correlation with the mechanism of algal blooms.; The phytochrome gene from Microcystis aeruginosa FACHB-912, isolated from algal blooms in Taihu Lake in China, was sequenced and found to code for a wavelength transition light receptor protein. The gene was composed of five distinct domains: a PAS fold domain, a GAF domain, a phytochrome domain, a His Kinase A (phosphoacceptor) domain, and a histidine kinase domain. Red-absorbing phytochrome, far-red-absorbing phytochrome, and photoconversion kinetics were monitored via the spectral characteristics of the protein. To detect positive selection, homologous sequences from cyanobacterium phytochrome genes were obtained from GenBank. Potential selective pressure was identified by phylogenetic analysis with maximum likelihood analyses. The frequency of the phytochrome gene being subjected to selective constraints suggests that positive selection is a potentially important mechanism that promotes the evolution of the cyanobacteria phytochrome gene. The sites identified in this study provide targets for further research on the structural-functional role of these residues, and on the correlation with the mechanism of algal blooms.
文章类型Article
资助机构CAS/SAFEA International Partnership Program for Creative Research Teams; Chinese Marine Microbial Center[KSCX2-YW-G-073]
收录类别SCI
语种英语
关键词[WOS]CODON-SUBSTITUTION MODELS ; ASPEN POPULUS-TREMULA ; AMINO-ACID SITES ; PHYLOGENETIC ANALYSIS ; MOLECULAR EVOLUTION ; ADAPTIVE EVOLUTION ; HISTIDINE KINASES ; NATURAL VARIATION ; LIGHT ; BACTERIOPHYTOCHROMES
研究领域[WOS]Science & Technology - Other Topics
WOS记录号WOS:000307750500004
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6138
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Korean Ocean Res & Dev Inst, Marine Biotechnol Res Ctr, Ansan 425600, South Korea
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
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Shi Fei,Li Nan,Liu ShaoFang,et al. Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria[J]. CHINESE SCIENCE BULLETIN,2012,57(25):3270-3275.
APA Shi Fei,Li Nan,Liu ShaoFang,&Qin Song.(2012).Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria.CHINESE SCIENCE BULLETIN,57(25),3270-3275.
MLA Shi Fei,et al."Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria".CHINESE SCIENCE BULLETIN 57.25(2012):3270-3275.
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