Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria
Shi Fei1,3; Li Nan1,2,3; Liu ShaoFang3,4; Qin Song1
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
2012-09-01
Volume57Issue:25Pages:3270-3275
KeywordWater Bloom Phytochrome Microcystis Aeruginosa Positive Selection Cyanobacteria Spectral Characteristic
Contribution Rank[Shi Fei; Li Nan; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Li Nan] Korean Ocean Res & Dev Inst, Marine Biotechnol Res Ctr, Ansan 425600, South Korea; [Shi Fei; Li Nan; Liu ShaoFang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Liu ShaoFang] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Corresponding AuthorQin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.,sqin@yic.ac.cn
Department海岸带生物学与生物资源利用所重点实验室
AbstractThe phytochrome gene from Microcystis aeruginosa FACHB-912, isolated from algal blooms in Taihu Lake in China, was sequenced and found to code for a wavelength transition light receptor protein. The gene was composed of five distinct domains: a PAS fold domain, a GAF domain, a phytochrome domain, a His Kinase A (phosphoacceptor) domain, and a histidine kinase domain. Red-absorbing phytochrome, far-red-absorbing phytochrome, and photoconversion kinetics were monitored via the spectral characteristics of the protein. To detect positive selection, homologous sequences from cyanobacterium phytochrome genes were obtained from GenBank. Potential selective pressure was identified by phylogenetic analysis with maximum likelihood analyses. The frequency of the phytochrome gene being subjected to selective constraints suggests that positive selection is a potentially important mechanism that promotes the evolution of the cyanobacteria phytochrome gene. The sites identified in this study provide targets for further research on the structural-functional role of these residues, and on the correlation with the mechanism of algal blooms.; The phytochrome gene from Microcystis aeruginosa FACHB-912, isolated from algal blooms in Taihu Lake in China, was sequenced and found to code for a wavelength transition light receptor protein. The gene was composed of five distinct domains: a PAS fold domain, a GAF domain, a phytochrome domain, a His Kinase A (phosphoacceptor) domain, and a histidine kinase domain. Red-absorbing phytochrome, far-red-absorbing phytochrome, and photoconversion kinetics were monitored via the spectral characteristics of the protein. To detect positive selection, homologous sequences from cyanobacterium phytochrome genes were obtained from GenBank. Potential selective pressure was identified by phylogenetic analysis with maximum likelihood analyses. The frequency of the phytochrome gene being subjected to selective constraints suggests that positive selection is a potentially important mechanism that promotes the evolution of the cyanobacteria phytochrome gene. The sites identified in this study provide targets for further research on the structural-functional role of these residues, and on the correlation with the mechanism of algal blooms.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordCODON-SUBSTITUTION MODELS ; ASPEN POPULUS-TREMULA ; AMINO-ACID SITES ; PHYLOGENETIC ANALYSIS ; MOLECULAR EVOLUTION ; ADAPTIVE EVOLUTION ; HISTIDINE KINASES ; NATURAL VARIATION ; LIGHT ; BACTERIOPHYTOCHROMES
WOS Research AreaScience & Technology - Other Topics
Funding ProjectShi Fei; Li Nan; Liu ShaoFang; Qin Song
WOS IDWOS:000307750500004
Funding OrganizationCAS/SAFEA International Partnership Program for Creative Research Teams; Chinese Marine Microbial Center[KSCX2-YW-G-073]
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6138
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Korean Ocean Res & Dev Inst, Marine Biotechnol Res Ctr, Ansan 425600, South Korea
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Shi Fei,Li Nan,Liu ShaoFang,et al. Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria[J]. CHINESE SCIENCE BULLETIN,2012,57(25):3270-3275.
APA Shi Fei,Li Nan,Liu ShaoFang,&Qin Song.(2012).Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria.CHINESE SCIENCE BULLETIN,57(25),3270-3275.
MLA Shi Fei,et al."Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria".CHINESE SCIENCE BULLETIN 57.25(2012):3270-3275.
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