海岸带生物学与生物资源保护实验室知识库

QQ客服 官方微博 反馈留言

	Revealing the Diversity and Quantity of Peritrich Ciliates in Environmental Samples Using Specific Primer-based PCR and Quantitative PCR
	Liu, Xihan1,3; Gong, Jun1,2
发表期刊	MICROBES AND ENVIRONMENTS
ISSN	1342-6311
	2012-12-07
卷号	27期号:4页码:497-503
关键词	Ciliophora Peritricha Qpcr Specific Primers Ssu Rrna Gene Copy Number
产权排序	[Liu, Xihan; Gong, Jun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Environm Microbiol, Yantai 264003, Peoples R China; [Gong, Jun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Liu, Xihan] Ocean Univ China, Inst Evolut & Marine Divers, Qingdao 266003, Peoples R China
通讯作者	Gong, J (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Environm Microbiol, Yantai 264003, Peoples R China.
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	Peritrichs are a diverse, ecologically important ciliate group usually with a complex life cycle. To date, the community of the peritrichs has been investigated by using morphology-based methods such as living observation and silver staining. Here we show a molecular approach for characterizing the diversity and quantity of free-living peritrichs in environmental samples. We newly designed four peritrich-specific primers targeting 18S rRNA genes that allow clone library construction, screening and analysis. A quantitative real-time PCR (qPCR) assay was developed to quantify peritrichs in environmental samples by using rDNA copy number as an indicator. DNA extracted from four water samples of contrasting environmental gradients was analysed. The results showed that the peritrich community was differentiated among these samples, and that the diversity decreased with the increase of water salinity. The qPCR results are consistent with the library sequence analysis in terms of quantity variations from sample to sample. The development of peritrich-specific primers, for the first time, for conventional PCR and qPCR assays, provides useful molecular tools for revealing the diversity and quantity of peritrich ciliates in environmental samples. Also, our study illustrates the potential of these molecular tools to ecological studies of other ciliate groups in diverse environments.; Peritrichs are a diverse, ecologically important ciliate group usually with a complex life cycle. To date, the community of the peritrichs has been investigated by using morphology-based methods such as living observation and silver staining. Here we show a molecular approach for characterizing the diversity and quantity of free-living peritrichs in environmental samples. We newly designed four peritrich-specific primers targeting 18S rRNA genes that allow clone library construction, screening and analysis. A quantitative real-time PCR (qPCR) assay was developed to quantify peritrichs in environmental samples by using rDNA copy number as an indicator. DNA extracted from four water samples of contrasting environmental gradients was analysed. The results showed that the peritrich community was differentiated among these samples, and that the diversity decreased with the increase of water salinity. The qPCR results are consistent with the library sequence analysis in terms of quantity variations from sample to sample. The development of peritrich-specific primers, for the first time, for conventional PCR and qPCR assays, provides useful molecular tools for revealing the diversity and quantity of peritrich ciliates in environmental samples. Also, our study illustrates the potential of these molecular tools to ecological studies of other ciliate groups in diverse environments.
文章类型	Article
资助机构	One Hundred Talent Program of CAS; Natural Science Foundation of China [40976099, 41176143]; FANEDD [2007B27]
收录类别	SCI
语种	英语
关键词[WOS]	RIBOSOMAL-RNA GENE ; REAL-TIME PCR ; MOLECULAR CHARACTERIZATION ; CILIOPHORA PERITRICHIA ; BIOFILM COMMUNITIES ; PLANKTONIC CILIATE ; NORTH CHINA ; OLIGOHYMENOPHOREA ; PROTOZOA ; QUANTIFICATION
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000312162800021
引用统计	被引频次：17[WOS]   [WOS记录]     [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/6136
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Environm Microbiol, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Ocean Univ China, Inst Evolut & Marine Divers, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xihan,Gong, Jun. Revealing the Diversity and Quantity of Peritrich Ciliates in Environmental Samples Using Specific Primer-based PCR and Quantitative PCR[J]. MICROBES AND ENVIRONMENTS,2012,27(4):497-503.
APA	Liu, Xihan,&Gong, Jun.(2012).Revealing the Diversity and Quantity of Peritrich Ciliates in Environmental Samples Using Specific Primer-based PCR and Quantitative PCR.MICROBES AND ENVIRONMENTS,27(4),497-503.
MLA	Liu, Xihan,et al."Revealing the Diversity and Quantity of Peritrich Ciliates in Environmental Samples Using Specific Primer-based PCR and Quantitative PCR".MICROBES AND ENVIRONMENTS 27.4(2012):497-503.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Revealing the Divers（419KB）			开放获取	--	浏览 请求全文

个性服务

推荐该条目

保存到收藏夹

查看访问统计

导出为Endnote文件

谷歌学术

谷歌学术中相似的文章

[Liu, Xihan]的文章

[Gong, Jun]的文章

百度学术

百度学术中相似的文章

[Liu, Xihan]的文章

[Gong, Jun]的文章

必应学术

必应学术中相似的文章

[Liu, Xihan]的文章

[Gong, Jun]的文章

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。