Proteomic response of wheat embryos to fosthiazate stress in a protected vegetable soil
Yin, Chunyan1,2; Teng, Ying1,2; Luo, Yongming1,2,3; Christie, Peter4
发表期刊JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
ISSN1001-0742
2012
卷号24期号:10页码:1843-1853
关键词Fosthiazate Proteomic Wheat Embryo Stress Response Biological Indicator
产权排序[Yin, Chunyan; Teng, Ying; Luo, Yongming] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Yin, Chunyan; Teng, Ying; Luo, Yongming] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Luo, Yongming] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Christie, Peter] Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland
通讯作者Luo, YM (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China.,cyyin@issas.ac.cn ; ymluo@yic.ac.cn
作者部门中科院海岸带环境过程与生态修复重点实验室
英文摘要A proteomic analysis of wheat defense response induced by the widely used organophosphorus nematicide fosthiazate is reported. Seed germination and two-dimensional gel electrophoresis (2-DE) experiments were performed using a Chinese wheat cultivar, Zhenmai No. 5. Root and shoot elongation decreased but thiobarbituric acid reactive substances (TBARS) content in embryos increased with increasing pesticide concentration. More than 1000 protein spots were reproducibly detected in each silver-stained gel. Thirty-seven protein spots with at least 2-fold changes were identified using MALDI-TOF MS/MS analysis. Of these, 24 spots were up-regulated and 13 were down-regulated. Proteins identified included some well-known classical stress responsive proteins under abiotic or biotic stresses as well as some unusual responsive proteins. Ten responsive proteins were reported for the first time at the proteomic level, including fatty acyl CoA reductase, dihydrodipicolinate synthase, DEAD-box ATPase-RNA-helicase, fimbriata-like protein, waxy B1, rust resistance kinase Lr10, putative 12.1 protein, retinoblastoma-related protein 1, pollen allergen-like protein and S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferase. The proteins identified were involved in several processes such as metabolism, defense/detoxification, cell structure/cell growth, signal transduction/transcription, photosynthesis and energy. Seven candidate proteins were further analyzed at the mRNA level by RT-PCR to compare transcript and protein accumulation patterns, revealing that not all the genes were correlated well with the protein level. Identification of these responsive proteins may provide new insight into the molecular basis of the fosthiazate-stress response in the early developmental stages of plants and may be useful in stress monitoring or stress-tolerant crop breeding for environmentally friendly agricultural production.; A proteomic analysis of wheat defense response induced by the widely used organophosphorus nematicide fosthiazate is reported. Seed germination and two-dimensional gel electrophoresis (2-DE) experiments were performed using a Chinese wheat cultivar, Zhenmai No. 5. Root and shoot elongation decreased but thiobarbituric acid reactive substances (TBARS) content in embryos increased with increasing pesticide concentration. More than 1000 protein spots were reproducibly detected in each silver-stained gel. Thirty-seven protein spots with at least 2-fold changes were identified using MALDI-TOF MS/MS analysis. Of these, 24 spots were up-regulated and 13 were down-regulated. Proteins identified included some well-known classical stress responsive proteins under abiotic or biotic stresses as well as some unusual responsive proteins. Ten responsive proteins were reported for the first time at the proteomic level, including fatty acyl CoA reductase, dihydrodipicolinate synthase, DEAD-box ATPase-RNA-helicase, fimbriata-like protein, waxy B1, rust resistance kinase Lr10, putative 12.1 protein, retinoblastoma-related protein 1, pollen allergen-like protein and S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferase. The proteins identified were involved in several processes such as metabolism, defense/detoxification, cell structure/cell growth, signal transduction/transcription, photosynthesis and energy. Seven candidate proteins were further analyzed at the mRNA level by RT-PCR to compare transcript and protein accumulation patterns, revealing that not all the genes were correlated well with the protein level. Identification of these responsive proteins may provide new insight into the molecular basis of the fosthiazate-stress response in the early developmental stages of plants and may be useful in stress monitoring or stress-tolerant crop breeding for environmentally friendly agricultural production.
文章类型Article
资助机构National Environmental Protection special funds for scientific research on public causes of China [2010467016]; Natural Science Foundation of Jiangsu Province [BK2009016]; Chinese Academy of Sciences [KZCX2-YW-Q02-06-02, KZCW2-YW-404]
收录类别SCI
语种英语
关键词[WOS]NON-FUMIGANT NEMATICIDES ; HEAT-SHOCK PROTEINS ; TRITICUM-AESTIVUM ; OXIDATIVE STRESS ; N-METHYLTRANSFERASE ; TUBULIN GENES ; CELL-DIVISION ; SALT STRESS ; RICE ; PLANTS
研究领域[WOS]Environmental Sciences & Ecology
WOS记录号WOS:000310408700016
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6038
专题中国科学院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
4.Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland
推荐引用方式
GB/T 7714
Yin, Chunyan,Teng, Ying,Luo, Yongming,et al. Proteomic response of wheat embryos to fosthiazate stress in a protected vegetable soil[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2012,24(10):1843-1853.
APA Yin, Chunyan,Teng, Ying,Luo, Yongming,&Christie, Peter.(2012).Proteomic response of wheat embryos to fosthiazate stress in a protected vegetable soil.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,24(10),1843-1853.
MLA Yin, Chunyan,et al."Proteomic response of wheat embryos to fosthiazate stress in a protected vegetable soil".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 24.10(2012):1843-1853.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Proteomic response o(1875KB) 开放获取--浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yin, Chunyan]的文章
[Teng, Ying]的文章
[Luo, Yongming]的文章
百度学术
百度学术中相似的文章
[Yin, Chunyan]的文章
[Teng, Ying]的文章
[Luo, Yongming]的文章
必应学术
必应学术中相似的文章
[Yin, Chunyan]的文章
[Teng, Ying]的文章
[Luo, Yongming]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Proteomic response of wheat embryos to fosthiazate stress in a protected vegetable soil.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。