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| Biocompatible Triplex Ag@SiO2@mTiO2 Core-Shell Nanoparticles for Simultaneous Fluorescence-SERS Bimodal Imaging and Drug Delivery | |
Wang, Yunqing ; Chen, Lingxin; Liu, Ping
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| 发表期刊 | CHEMISTRY-A EUROPEAN JOURNAL
 |
| ISSN | 0947-6539 |
| 2012-05-01 | |
| 卷号 | 18期号:19页码:5935-5943 |
| 关键词 | Core-shell Structures Drug Delivery Fluorescence Nanoparticles Surface-enhanced Raman Scattering |
| 产权排序 | [Wang, Yunqing; Chen, Lingxin; Liu, Ping] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China.,lxchen@yic.ac.cn |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Herein, we report the synthesis of biocompatible triplex Ag@SiO2@mTiO2 coreshell nanoparticles (NPs) for simultaneous fluorescence-surface-enhanced Raman scattering (F-SERS) bimodal imaging and drug delivery. Stable Raman signals were created by typical SERS tags that were composed of Ag NPs for optical enhancement, a reporter molecule of 4-mercaptopyridine (4-Mpy) for a spectroscopic signature, and a silica shell for protection. A further coating of mesoporous titania (mTiO2) on the SERS tags offered high loading capacity for a fluorescence dye (flavin mononucleotide) and an anti-cancer drug (doxorubicin (DOX)), thereby endowing the material with fluorescence-imaging and therapeutic functions. The as-prepared F-SERS dots exhibited strong fluorescence when excited by light at 460 nm whilst a stable, characteristic 4-Mpy SERS signal was detected when the excitation wavelength was changed to longer wavelength (632.8 nm), both in solution and after incorporation inside living cells. Their excellent biocompatibility was demonstrated by low cytotoxicity against MCF-7 cells, even at a high concentration of 100 mu g?mL-1. In vitro cell cytotoxicity confirmed that DOX-loaded F-SERS dots had a comparable or even greater therapeutic effect compared with the free drug, owing to the increased cell-uptake, which was attributed to the possible endocytosis mechanism of the NPs. To the best of our knowledge, this is the first proof-of-concept investigation on a multifunctional nanomedicine that possessed a combined capacity for fast and multiplexed F-SERS labeling as well as drug-loading for cancer therapy.; Herein, we report the synthesis of biocompatible triplex Ag@SiO2@mTiO2 coreshell nanoparticles (NPs) for simultaneous fluorescence-surface-enhanced Raman scattering (F-SERS) bimodal imaging and drug delivery. Stable Raman signals were created by typical SERS tags that were composed of Ag NPs for optical enhancement, a reporter molecule of 4-mercaptopyridine (4-Mpy) for a spectroscopic signature, and a silica shell for protection. A further coating of mesoporous titania (mTiO2) on the SERS tags offered high loading capacity for a fluorescence dye (flavin mononucleotide) and an anti-cancer drug (doxorubicin (DOX)), thereby endowing the material with fluorescence-imaging and therapeutic functions. The as-prepared F-SERS dots exhibited strong fluorescence when excited by light at 460 nm whilst a stable, characteristic 4-Mpy SERS signal was detected when the excitation wavelength was changed to longer wavelength (632.8 nm), both in solution and after incorporation inside living cells. Their excellent biocompatibility was demonstrated by low cytotoxicity against MCF-7 cells, even at a high concentration of 100 mu g?mL-1. In vitro cell cytotoxicity confirmed that DOX-loaded F-SERS dots had a comparable or even greater therapeutic effect compared with the free drug, owing to the increased cell-uptake, which was attributed to the possible endocytosis mechanism of the NPs. To the best of our knowledge, this is the first proof-of-concept investigation on a multifunctional nanomedicine that possessed a combined capacity for fast and multiplexed F-SERS labeling as well as drug-loading for cancer therapy. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China[20975089, 81102415]; Natural Science Foundation of Shandong Province of China[ZR2010BQ012]; Science and Technology Development Plan of Yantai[2011071]; Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ENHANCED RAMAN-SCATTERING ; CANCER-CELLS ; SILVER NANOPARTICLES ; PHOTOTHERMAL THERAPY ; SPECTROSCOPIC DOTS ; TIO2 PARTICLES ; TAGS ; 4-MERCAPTOPYRIDINE ; NANOCRYSTALS ; IMMUNOASSAY |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000303497100018 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5962 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yunqing,Chen, Lingxin,Liu, Ping. Biocompatible Triplex Ag@SiO2@mTiO2 Core-Shell Nanoparticles for Simultaneous Fluorescence-SERS Bimodal Imaging and Drug Delivery[J]. CHEMISTRY-A EUROPEAN JOURNAL,2012,18(19):5935-5943. |
| APA | Wang, Yunqing,Chen, Lingxin,&Liu, Ping.(2012).Biocompatible Triplex Ag@SiO2@mTiO2 Core-Shell Nanoparticles for Simultaneous Fluorescence-SERS Bimodal Imaging and Drug Delivery.CHEMISTRY-A EUROPEAN JOURNAL,18(19),5935-5943. |
| MLA | Wang, Yunqing,et al."Biocompatible Triplex Ag@SiO2@mTiO2 Core-Shell Nanoparticles for Simultaneous Fluorescence-SERS Bimodal Imaging and Drug Delivery".CHEMISTRY-A EUROPEAN JOURNAL 18.19(2012):5935-5943. |
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