Determination of total sugar can serve to monitoring the fermentation process of Jerusalem artichoke (Helianthus tuberosus). Anthrone colorimetric method was established for the total sugar determination, followed by the study of the effect of ethanol, butyric acid, lactic acid, 1,4-butanediol on the determination of total sugar. The results show that the established determination method of total sugar is stable and repeatable. In addition, ethanol and butyric acid (less than 10%, v/v) have no effect on the determination of total sugar. However, lactic acid and 1,4-butanediol have great effect on the determination of total sugar. Interestingly, the absorbance of inulin solution with lactic acid and 1,4- butanediol after anthrone reaction equals to the sum of the absorbance of inulin solution after anthrone reaction and the absorbance of lactic acid or 1,4-butanediol solution after anthrone reaction.
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