Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial
He, Weiyu1; Feng, Feifei1; Huang, Yong4; Guo, Huanhuan1; Zhang, Songyan2; Li, Zheng2; Liu, Jingze1; Wang, Yipeng3; Yu, Haining1,2
发表期刊BIOCHIMIE
ISSN0300-9084
2012-03-01
卷号94期号:3页码:649-655
关键词Amphibian Diversity Anti-microbial Peptide (Amp) Antioxidant Odorrana Tiannanensis
产权排序[He, Weiyu; Feng, Feifei; Guo, Huanhuan; Liu, Jingze; Yu, Haining] Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China; [Zhang, Songyan; Li, Zheng; Yu, Haining] Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China; [Wang, Yipeng] Chinese Acad Sci, Biol Resources Lab, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Huang, Yong] Guangxi Bot Garden Med Plants, Nanning 530023, Peoples R China
通讯作者Yu, HN (reprint author), Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China. wyp010@163.com ; joannyu@live.cn
作者部门生物资源实验室 
英文摘要Genus Odorrana, among all amphibians studied, is generally reported to have the most abundant and diversified anti-microbial peptides even from a single individual frog. In our previous work, 46 cDNA sequences encoding precursors of 22 different anti-microbial peptides (AMPs) were characterized from the skin of frog, Odorrana tiannanensis. In this work, we reported the purification of three AMPs from skin secretions of O. tiannanensis. Their amino acid sequences matched well with the sequences deduced from cDNAs and they were designated as Odorranain-C7HSa, Brevinin-1-OT2 and Odorranain-G-OT, respectively. Furthermore, we selected to analyze the four most structurally diversified sequences among the 22 AMPs that are significantly different from all reported AMPs. By structural characterization, three of them were designated as pleurain-E-OT, odorranain-G-OT, odorranain-A-OT, belonging to AMP families already identified. The forth one with a unique 14-mer sequence of AILTTLANWARKFLa and C-terminal amidation represents the prototypes of a new class of amphibian AMP, and thereby named tiannanensin. Such broad diversity in sequences and structures are consistent with other species in Genus Odorrana. Multi-functions of the synthesized four special AMPs were screened, including anti-microbial, antioxidant, cytotoxic and hemolytic activities. The results suggest that these AMPs may employ sophisticated mechanisms of action in host defense in addition to anti-microbial, although their precise contribution to host defense still seems unclear.; Genus Odorrana, among all amphibians studied, is generally reported to have the most abundant and diversified anti-microbial peptides even from a single individual frog. In our previous work, 46 cDNA sequences encoding precursors of 22 different anti-microbial peptides (AMPs) were characterized from the skin of frog, Odorrana tiannanensis. In this work, we reported the purification of three AMPs from skin secretions of O. tiannanensis. Their amino acid sequences matched well with the sequences deduced from cDNAs and they were designated as Odorranain-C7HSa, Brevinin-1-OT2 and Odorranain-G-OT, respectively. Furthermore, we selected to analyze the four most structurally diversified sequences among the 22 AMPs that are significantly different from all reported AMPs. By structural characterization, three of them were designated as pleurain-E-OT, odorranain-G-OT, odorranain-A-OT, belonging to AMP families already identified. The forth one with a unique 14-mer sequence of AILTTLANWARKFLa and C-terminal amidation represents the prototypes of a new class of amphibian AMP, and thereby named tiannanensin. Such broad diversity in sequences and structures are consistent with other species in Genus Odorrana. Multi-functions of the synthesized four special AMPs were screened, including anti-microbial, antioxidant, cytotoxic and hemolytic activities. The results suggest that these AMPs may employ sophisticated mechanisms of action in host defense in addition to anti-microbial, although their precise contribution to host defense still seems unclear. (C) 2011 Elsevier Masson SAS. All rights reserved.
文章类型Article
资助机构Chinese National Natural Science Foundation[30900240, 41076098]
收录类别SCI
语种英语
关键词[WOS]AMPHIBIAN SKIN ; PEPTIDOMICS ; INDUCTION ; ROLES
研究领域[WOS]Biochemistry & Molecular Biology
WOS记录号WOS:000301332200008
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/5561
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China
2.Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Biol Resources Lab, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
4.Guangxi Bot Garden Med Plants, Nanning 530023, Peoples R China
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He, Weiyu,Feng, Feifei,Huang, Yong,et al. Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial[J]. BIOCHIMIE,2012,94(3):649-655.
APA He, Weiyu.,Feng, Feifei.,Huang, Yong.,Guo, Huanhuan.,Zhang, Songyan.,...&Yu, Haining.(2012).Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial.BIOCHIMIE,94(3),649-655.
MLA He, Weiyu,et al."Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial".BIOCHIMIE 94.3(2012):649-655.
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