Applications of molecular biology and biotechnology in oil field microbial biodiversity research
Wen, Zhuang1,2,4; Hong-Bo, Shao1,3
发表期刊AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
ISSN1996-0808
2011-09-30
卷号5期号:20页码:3103-3112
关键词Biotechnology Molecular Biology Techniques Oil Field 16s Rrna Microbial Biodiversity Prospect
产权排序[Wen, Zhuang; Hong-Bo, Shao] Chinese Acad Sci CAS, CAS Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wen, Zhuang] Zaozhuang Vocat Coll, Zaozhuang 277800, Peoples R China; [Hong-Bo, Shao] Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China; [Wen, Zhuang] Chinese Acad Sci CAS, Grad Univ, Beijing 100049, Peoples R China
通讯作者Hong-Bo, S (reprint author), Chinese Acad Sci CAS, CAS Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
作者部门滨海湿地实验室 
英文摘要In recent years, with more and more attention given to the study of microorganisms in oil fields, molecular biology and biotechnology methodology has been applied prosperously. This article described the most commonly-used molecular biology techniques and related biotechnological advances in oil fields research at present, which are based mainly on 16s rRNA, such as 16S rRNA sequencing, denaturant gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP), etc. The article also points out their advantages and disadvantages, respectively and introduces some of the micro-organisms, which are of largest concerns in the research.; In recent years, with more and more attention given to the study of microorganisms in oil fields, molecular biology and biotechnology methodology has been applied prosperously. This article described the most commonly-used molecular biology techniques and related biotechnological advances in oil fields research at present, which are based mainly on 16s rRNA, such as 16S rRNA sequencing, denaturant gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP), etc. The article also points out their advantages and disadvantages, respectively and introduces some of the micro-organisms, which are of largest concerns in the research.
文章类型Review
资助机构Chinese Academy of Sciences (CAS)[KZCX2-YW-JC203, 2009Y2B211]; CAS/SAFEA
收录类别SCI
语种英语
关键词[WOS]GRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; SULFATE-REDUCING BACTERIA ; ACTIVATED-SLUDGE ; H2S PRODUCTION ; DNA FRAGMENTS ; PCR ; DIVERSITY ; POPULATIONS ; COMMUNITY
研究领域[WOS]Microbiology
WOS记录号WOS:000298925600002
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/5302
专题中国科学院海岸带环境过程与生态修复重点实验室_滨海湿地实验室
作者单位1.Chinese Acad Sci CAS, CAS Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Zaozhuang Vocat Coll, Zaozhuang 277800, Peoples R China
3.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China
4.Chinese Acad Sci CAS, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Wen, Zhuang,Hong-Bo, Shao. Applications of molecular biology and biotechnology in oil field microbial biodiversity research[J]. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,2011,5(20):3103-3112.
APA Wen, Zhuang,&Hong-Bo, Shao.(2011).Applications of molecular biology and biotechnology in oil field microbial biodiversity research.AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,5(20),3103-3112.
MLA Wen, Zhuang,et al."Applications of molecular biology and biotechnology in oil field microbial biodiversity research".AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH 5.20(2011):3103-3112.
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