| Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer | |
Li Jialin1,2 ; Bai Jie1; Gao Huiwang1; Liu Guangxing1
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| 发表期刊 | ACTA OCEANOLOGICA SINICA
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| ISSN | 0253-505X |
| 2011-05-01 | |
| 卷号 | 30期号:3页码:92-99 |
| 关键词 | Ammonia-oxidizing Betaproteobacteria (Beta Aob) Diversity Abundance Nitrification Surface Sediment Changjiang River Estuary (Cre) |
| 产权排序 | [Li, JL; Bai, J; Gao, HW; Liu, GX] Ocean Univ China, Coll Environm Sci & Engn, Minist Educ, Key Lab Marine Environm Sci & Ecol, Qingdao 266100, Peoples R China; [Li, JL] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 通讯作者 | Ocean Univ China, Coll Environm Sci & Engn, Minist Educ, Key Lab Marine Environm Sci & Ecol, Qingdao 266100, Peoples R China. baijie@ouc.edu.cn |
| 作者部门 | 生物资源实验室 |
| 英文摘要 | The spatial distribution of ammonia-oxidizing Betaproteobacteria (beta AOB) was investigated by FISH (fluorescence in situ hybridization) and DGGE (denaturing gradient gel electrophoresis) techniques in the sediment off the Changjiang River Estuary. Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006. The abundance of beta AOB ranged from 1.87 x 10(5) to 3.53 x 10(5) cells/g of sediment. beta AOB abundance did not present a negative correlation with salinity, whereas salinity was implicated as the primary factor affecting nitrification rates. The DGGE profiles of PCR-amplified amoA gene fragments revealed that the beta AOB community structure of sample S2 separated from other samples at the level of 40% similarity. The variations in composition of beta AOB were significantly correlated with the salinity, temperature, absorption ability of sediments and TOC. The statistical analysis indicates that the beta AOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity. Considering the good correlation between beta AOB abundance and nitrification estimates, the abundance and diversity of beta AOB community could be expected as an indirect index of nitrification activity at the study sea area in summer.; The spatial distribution of ammonia-oxidizing Betaproteobacteria (beta AOB) was investigated by FISH (fluorescence in situ hybridization) and DGGE (denaturing gradient gel electrophoresis) techniques in the sediment off the Changjiang River Estuary. Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006. The abundance of beta AOB ranged from 1.87 x 10(5) to 3.53 x 10(5) cells/g of sediment. beta AOB abundance did not present a negative correlation with salinity, whereas salinity was implicated as the primary factor affecting nitrification rates. The DGGE profiles of PCR-amplified amoA gene fragments revealed that the beta AOB community structure of sample S2 separated from other samples at the level of 40% similarity. The variations in composition of beta AOB were significantly correlated with the salinity, temperature, absorption ability of sediments and TOC. The statistical analysis indicates that the beta AOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity. Considering the good correlation between beta AOB abundance and nitrification estimates, the abundance and diversity of beta AOB community could be expected as an indirect index of nitrification activity at the study sea area in summer. |
| 文章类型 | Article |
| 资助机构 | National Fundamental Project of China[2006CB400602] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | EAST CHINA SEA ; 16S RIBOSOMAL-RNA ; ENVIRONMENTAL GRADIENTS ; SPATIAL-DISTRIBUTION ; NITRIFICATION RATES ; BACTERIA ; DIVERSITY ; ARCHAEA ; NITROGEN ; OCEAN |
| 研究领域[WOS] | Oceanography |
| WOS记录号 | WOS:000292673000011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5115 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Ocean Univ China, Coll Environm Sci & Engn, Minist Educ, Key Lab Marine Environm Sci & Ecol, Qingdao 266100, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Jialin,Bai Jie,Gao Huiwang,et al. Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer[J]. ACTA OCEANOLOGICA SINICA,2011,30(3):92-99. |
| APA | Li Jialin,Bai Jie,Gao Huiwang,&Liu Guangxing.(2011).Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer.ACTA OCEANOLOGICA SINICA,30(3),92-99. |
| MLA | Li Jialin,et al."Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer".ACTA OCEANOLOGICA SINICA 30.3(2011):92-99. |
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