| Isolation and Characterization of a Stress-Dependent Plastidial Delta(12) Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ-7 | |
Lu, Yandu1,2,3; Chi, Xiaoyuan4; Li, Zhaoxin5; Yang, Qingli4; Li, Fuchao2; Liu, Shaofang2,3; Gan, Qinhua6; Qin, Song1
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| 发表期刊 | LIPIDS
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| ISSN | 0024-4201 |
| 2010-02-01 | |
| 卷号 | 45期号:2页码:179-187 |
| 关键词 | Antarctic Chlorella Cold Stress Delta(12) Fatty Acid Desaturation Gene Regulation Salt Stress |
| 产权排序 | [Lu, Yandu; Qin, Song] Chinese Acad Sci, YanTai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Lu, Yandu; Li, Fuchao; Liu, Shaofang] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Lu, Yandu; Liu, Shaofang] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Chi, Xiaoyuan; Yang, Qingli] Shandong Peanut Res Inst, Qingdao 266100, Peoples R China; [Li, Zhaoxin] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China; [Gan, Qinhua] Shandong Entry Exit Inspect & Quarantine Bur, Qingdao 266001, Peoples R China |
| 通讯作者 | Qin, S, Chinese Acad Sci, YanTai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 作者部门 | 生物资源实验室 |
| 英文摘要 | An acclimation to the changing physicochemical conditions and high amount of Delta(12)-unsaturated fatty acids of the Antarctic Chlorella vulgaris NJ-7 prompted us to speculate about the involvement of Delta(12)-fatty acid desaturases (FAD) in its adaptation to the extremely unfavorable ambience. A full-length cDNA sequence, designated CvFAD6, was isolated from C. vulgaris NJ-7 via RT-PCR and RACE methods. Sequence alignment showed that the gene was homologous to corresponding Delta(12)-FAD from other eukaryotes. Phylogenetic analysis showed that it was grouped with plastidial Delta(12)-FAD with conserved histidine boxes. Yeast cells transformed with a plasmid construct containing CvFAD6 coding region accumulated a considerable amount of linoleic acid (18:2 Delta(9,12)), normally not present in wild-type yeast cells, suggesting that the isolated gene encodes a functional Delta(12) enzyme. The correlation between the accumulation of CvFAD6 and temperature has been examined by real time PCR. The analysis showed a constant expression of CvFAD6 from 25 to 15 A degrees C whereas a fourfold increased from 25 to 4 A degrees C. Moreover, CvFAD6 transcription was more sensitive to saline stress since a 20-fold increase at 6% NaCl was detected. Our data demonstrate that CvFAD6 is the enzyme responsible for the Delta(12) fatty acids desaturation involved in low temperature and high salinity acclimation for Antarctic C. vulgaris NJ-7.; An acclimation to the changing physicochemical conditions and high amount of Delta(12)-unsaturated fatty acids of the Antarctic Chlorella vulgaris NJ-7 prompted us to speculate about the involvement of Delta(12)-fatty acid desaturases (FAD) in its adaptation to the extremely unfavorable ambience. A full-length cDNA sequence, designated CvFAD6, was isolated from C. vulgaris NJ-7 via RT-PCR and RACE methods. Sequence alignment showed that the gene was homologous to corresponding Delta(12)-FAD from other eukaryotes. Phylogenetic analysis showed that it was grouped with plastidial Delta(12)-FAD with conserved histidine boxes. Yeast cells transformed with a plasmid construct containing CvFAD6 coding region accumulated a considerable amount of linoleic acid (18:2 Delta(9,12)), normally not present in wild-type yeast cells, suggesting that the isolated gene encodes a functional Delta(12) enzyme. The correlation between the accumulation of CvFAD6 and temperature has been examined by real time PCR. The analysis showed a constant expression of CvFAD6 from 25 to 15 A degrees C whereas a fourfold increased from 25 to 4 A degrees C. Moreover, CvFAD6 transcription was more sensitive to saline stress since a 20-fold increase at 6% NaCl was detected. Our data demonstrate that CvFAD6 is the enzyme responsible for the Delta(12) fatty acids desaturation involved in low temperature and high salinity acclimation for Antarctic C. vulgaris NJ-7. |
| 文章类型 | Article |
| 资助机构 | Key Innovative Project of Chinese Academy of Science [KSCX2-YW-G-002, KZCX2-YW-209, KZCX2-YW-225]; National Science Foundation of China [30870247, 30670165, 40876082]; High-Tech Research and Development Program of China [2006AA10A114]; National Key Basic Research and Development Project of China [2007CB116212]; Key Technology Research Project of Qingdao [07-1-4-16-nsh] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MULTIPLE SEQUENCE ALIGNMENT ; FUNCTIONAL-CHARACTERIZATION ; SACCHAROMYCES-CEREVISIAE ; MEMBRANE-FLUIDITY ; PAVLOVA-SALINA ; GENE ; EXPRESSION ; COLD ; IDENTIFICATION ; CLONING |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Nutrition & Dietetics |
| WOS记录号 | WOS:000274333300008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/3761 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, YanTai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Shandong Peanut Res Inst, Qingdao 266100, Peoples R China 5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 6.Shandong Entry Exit Inspect & Quarantine Bur, Qingdao 266001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Yandu,Chi, Xiaoyuan,Li, Zhaoxin,et al. Isolation and Characterization of a Stress-Dependent Plastidial Delta(12) Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ-7[J]. LIPIDS,2010,45(2):179-187. |
| APA | Lu, Yandu.,Chi, Xiaoyuan.,Li, Zhaoxin.,Yang, Qingli.,Li, Fuchao.,...&Qin, Song.(2010).Isolation and Characterization of a Stress-Dependent Plastidial Delta(12) Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ-7.LIPIDS,45(2),179-187. |
| MLA | Lu, Yandu,et al."Isolation and Characterization of a Stress-Dependent Plastidial Delta(12) Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ-7".LIPIDS 45.2(2010):179-187. |
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