Growth-associated biosynthesis of astaxanthin in heterotrophic Chlorella zofingiensis (Chlorophyta)

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	Growth-associated biosynthesis of astaxanthin in heterotrophic Chlorella zofingiensis (Chlorophyta)
	Wang, Y.1,2 ; Peng, J.2
发表期刊	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN	0959-3993
	2008-09-01
卷号	24 期号:9 页码:1915-1922
关键词	Astaxanthin Chlorella Zofingiensis Glucose Heterotrophic Culture
产权排序	Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev;Chinese Acad Sci, S China Sea Inst Oceanol
通讯作者	Wang, Y, Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Shandong, Peoples R China
作者部门	生物资源实验室
英文摘要	The green microalga Chlorella zofingiensis can produce the ketocarotenoid astaxanthin under heterotrophic culture conditions. Here we report the growth-associated biosynthesis of astaxanthin in this biotechnologically important alga. With glucose as sole carbon and energy source, C. zofinginesis grew fast in the dark with rapid exhaustion of nitrogen and carbon sources from media, leading to a high specific growth rate (0.034 h(-1)). Cultures started at a cell concentration of about 3.4 x 10(9) cells l(-1) reached, after 6 days, standing biomass values of 1.6 x 10(11) cells or 8.5 g dry weight l(-1). Surprisingly, the biosynthesis of astaxanthin was found to start at early exponential phase, independent of cessation of cell division. A general trend was observed that the culture conditions benefiting cell growth also benefited astaxanthin accumulation, indicating that astaxanthin was a growth-associated product in this alga. The maximum cell dry biomass and astaxanthin yield were 11.75 g l(-1) and 11.14 mg l(-1) (about 1 mg g(-1)), simultaneously obtained in the fed-batch culture with a combined glucose-nitrate mixture addition, which were the highest ever reported in dark-heterotrophic algal cultures. The possible reasons why dark-heterotrophic C. zofingiensis could produce astaxanthin during the course of cell growth were discussed.
文章类型	Article
收录类别	SCI
语种	英语
关键词[WOS]	HAEMATOCOCCUS-PLUVIALIS ; PHAFFIA-RHODOZYMA ; GREEN-ALGA ; CAROTENOID BIOSYNTHESIS ; SECONDARY CAROTENOIDS ; MIXOTROPHIC CULTURE ; CHLOROCOCCUM SP ; ACCUMULATION ; MICROALGAE ; STRESS
研究领域[WOS]	Biotechnology & Applied Microbiology
WOS记录号	WOS:000257961500036
引用统计	被引频次：40[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/3499
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Y.,Peng, J.. Growth-associated biosynthesis of astaxanthin in heterotrophic Chlorella zofingiensis (Chlorophyta)[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2008,24(9):1915-1922.
APA	Wang, Y.,&Peng, J..(2008).Growth-associated biosynthesis of astaxanthin in heterotrophic Chlorella zofingiensis (Chlorophyta).WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,24(9),1915-1922.
MLA	Wang, Y.,et al."Growth-associated biosynthesis of astaxanthin in heterotrophic Chlorella zofingiensis (Chlorophyta)".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 24.9(2008):1915-1922.

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