Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot<i> (Scophthalmus</i><i> maximus)</i>

doi:10.1016/j.fsi.2024.109412

YIC-IR

	Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot (Scophthalmus maximus)
	Zhang, Jian 1,3; Zhou, Dandan 1; Zhang, Binzhe 1; Li, Xuepeng 1; Jiao, Xudong2,4
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2024-03-01
卷号	146 页码:8
关键词	Scophthalmus maximus Tetraspanin CD53 Immunoregulation Antibody ligation
DOI	10.1016/j.fsi.2024.109412
通讯作者	Zhang, Jian(zhangjian@ytu.edu.cn) ; Jiao, Xudong(xdjiao@yic.ac.cn)
英文摘要	Cluster of differentiation 53 (CD53) also known as OX44 or tetraspanin 25 (TSPAN25) is a glycoprotein belonging to the tetraspanin family. Members of the tetraspanin family are characterized by four transmembrane domains, including intracellular N- and C-termini, and small and large extracellular domains. Currently, the function of CD53 in teleost is not well understood. In this study, we identified a CD53 (named SmCD53) from turbot (Scophthalmus maximus) and examined its expression and biological activity. SmCD53 contained 231 amino acid residues and was predicted to be a tetraspanin with small and large extracellular domains. SmCD53 expression was observed in different tissues, particularly in immune-related organs. Experimental infection with bacterial or viral pathogen significantly up-regulated SmCD53 expression in a time-dependent manner. Immunofluorescence microscopy analysis showed that SmCD53 was localized on the surface of PBL and was recognized by antibody against its large extracellular domain. Ligation of SmCD53 onto PBLs with antibodies suppressed the respiratory burst activity, inflammatory reaction, and enhanced cell viability. SmCD53 knockdown significantly enhanced bacterial dissemination and proliferation in turbot. Overall, these results underscore the importance of CD53 in the maintenance of the function and homeostasis of the immune system.
资助机构	Natural Science Foundation of Shandong Province ; Development Plan of Youth Innovation Team in Colleges and Universities of Shandong Province ; Yantai University Doctoral Start-up Foundation
收录类别	SCI
语种	英语
关键词[WOS]	TIME RT-PCR ; TYROSINE-PHOSPHATASE ; EXPRESSION ; MOLECULE ; NEUTROPHILS ; INDUCTION ; ANTI-CD53 ; BACTERIAL ; ANTIGEN
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:001175385300001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/34888
专题	中国科学院烟台海岸带研究所
通讯作者	Zhang, Jian; Jiao, Xudong
作者单位	1.Yantai Univ, Sch Ocean, Yantai, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China 3.Yantai Univ, 30 Qingquan Rd, Yantai 264005, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jian,Zhou, Dandan,Zhang, Binzhe,et al. Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot (Scophthalmus maximus)[J]. FISH & SHELLFISH IMMUNOLOGY,2024,146:8.
APA	Zhang, Jian,Zhou, Dandan,Zhang, Binzhe,Li, Xuepeng,&Jiao, Xudong.(2024).Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot (Scophthalmus maximus).FISH & SHELLFISH IMMUNOLOGY,146,8.
MLA	Zhang, Jian,et al."Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot (Scophthalmus maximus)".FISH & SHELLFISH IMMUNOLOGY 146(2024):8.