Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics

doi:10.1016/j.ecoenv.2022.114412

YIC-IR

	Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics
	Lu, Zhen 1,2,3; Wu, Sihan 2,3; Xiao, Zeyu 2,3; Song, Jun 2,3; Wu, Huifeng1 ; Peng, Xiao 2,3
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
	2023
卷号	249 页码:10
关键词	Polystyrene nanoplastics (PS -NPs) Mussel MicroRNAs (miRNA) Toxicological effects Metabolism
DOI	10.1016/j.ecoenv.2022.114412
通讯作者	Wu, Huifeng(hfwu@yic.ac.cn) ; Peng, Xiao(pengxiao_px@szu.edu.cn)
英文摘要	Polystyrene nanoplastics (PS-NPs) are typical accumulated nanoplastics in the marine environment and organ-isms, and have strong potential risks to marine ecological environment and human health. MiRNAs could respond to and participate in the response process of environmental stressors. However, the response of miRNAs to nanoplastics has not been fully explored. In this study, miRNA responses of digestive glands in mussels Mytilus galloprovincialis treated by 200 nm PS-NPs (20, 200, 2000 mu g/L) for 7 days were characterized by BGISEQ-500 deep sequencing and bioinformatics analysis, along with histopathological quantification with planimetric pa-rameters on hematoxylin and eosin (H&E) staining. Results showed that one novel miRNA (novel_mir63) and seven known miRNAs (miR-34_2, miR-34_5, miR-281_8, let-7-5p_6, miR-10, miR-124, miR-29b-3p) were significantly (adjusted P-value < 0.05) differentially expressed after PS-NPs treatments, and most of them were down-regulated expect for novel_mir63 and miR-34_2. Function analysis of target genes corresponding to these differentially expressed miRNAs indicated that PS-NPs disturbed the process related to metabolism, aging, car-diac function, neural excitation, and repairment. Among them, acetyl-CoA C-acetyltransferase and purine metabolism pathway played vital connection roles. Meanwhile, significantly morphology changes of digestive tubes obtained from H&E stained sections also implied severely disrupted metabolic capability in digestive glands, reflected by significantly increased mean diverticular radius (MDR) and mean luminal radius (MLR) values and the ratio of MLR to mean epithelial thickness (MET), and significantly decreased MET value and MET/ MDR. Overall, these findings have revealed new characterization of miRNAs and their target genes in mussel M. galloprovincialis under PS-NPs stress, and provide important clues to further elucidate the toxicity mechanisms of PS-NPs.
资助机构	National Natural Science Foundation of China ; Guangdong Basic and Applied Basic Research Foundation ; China Post- doctoral Science Foundation
收录类别	SCI
语种	英语
关键词[WOS]	QUANTITATIVE ALTERATIONS ; MERCENARIA-MERCENARIA ; LITTORINA-LITTOREA ; PLASTIC DEBRIS ; MICROPLASTICS ; NANOPARTICLES ; ACCUMULATION ; METABOLISM ; BEHAVIOR ; CELL
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
WOS记录号	WOS:000906373300001
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/34822
专题	中国科学院烟台海岸带研究所
通讯作者	Wu, Huifeng; Peng, Xiao
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, YICCAS, Yantai 264003, Peoples R China 2.Shenzhen Univ, Ctr Biomed Opt & Photon CBOP, Shenzhen 518060, Peoples R China 3.Shenzhen Univ, Coll Phys & Optoelect Engn, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China
推荐引用方式 GB/T 7714	Lu, Zhen,Wu, Sihan,Xiao, Zeyu,et al. Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2023,249:10.
APA	Lu, Zhen,Wu, Sihan,Xiao, Zeyu,Song, Jun,Wu, Huifeng,&Peng, Xiao.(2023).Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,249,10.
MLA	Lu, Zhen,et al."Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 249(2023):10.